A5074148696- MicroRNA in disease regulation
- Particle physics theoretical and experimental studies
- Quantum Chromodynamics and Particle Interactions
- Circular RNAs in diseases
- High-Energy Particle Collisions Research
- Nuclear physics research studies
- Cancer-related molecular mechanisms research
- Nuclear Physics and Applications
- Superconducting Materials and Applications
- RNA Interference and Gene Delivery
- Particle Accelerators and Free-Electron Lasers
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Advanced Chemical Physics Studies
- Atomic and Molecular Physics
- Radiation Detection and Scintillator Technologies
- Dark Matter and Cosmic Phenomena
- Gamma-ray bursts and supernovae
- Medical Imaging Techniques and Applications
- Extracellular vesicles in disease
- Particle Detector Development and Performance
- Atomic and Subatomic Physics Research
- Geophysics and Gravity Measurements
- Particle accelerators and beam dynamics
- Scientific Research and Discoveries
Heinrich Heine University Düsseldorf
2014-2025
Düsseldorf University Hospital
2011-2025
European Organization for Nuclear Research
2000-2025
Vita-Salute San Raffaele University
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2023
RWTH Aachen University
2020
Universitätsklinikum Aachen
2020
University of Genoa
1997-2015
Heidelberg University
2006-2014
University Hospital Heidelberg
2013-2014
Mesenchymal stem cells (MSC) comprise a promising tool for cellular therapy. These are usually culture expanded prior to their application. However, precise molecular definition of MSC and the sequel long-term in vitro yet unknown. In this study, we have addressed impact replicative senescence on human preparations. Within 43 77 days cultivation (7 12 passages), demonstrated morphological abnormalities, enlargement, attenuated expression specific surface markers, ultimately proliferation...
Liver fibrosis is orchestrated by a complex network of signaling pathways regulating the deposition extracellular matrix proteins during fibrogenesis. MicroRNAs (miRNAs) represent family small noncoding RNAs controlling translation and transcription many genes. Recently, miRNAs have been suggested to crucially modulate cellular processes in liver such as hepatocarcinogenesis. However, their role not well understood. We systematically analyzed regulation mouse model carbon...
The Advanced GAmma Tracking Array (AGATA) is a European project to develop and operate the next generation gamma-ray spectrometer. AGATA based on technique of energy tracking in electrically segmented high-purity germanium crystals. This requires accurate determination energy, time position every interaction as gamma ray deposits its within detector volume. Reconstruction full path results with very high efficiency excellent spectral response. realization result many technical advances....
MicroRNAs represent a class of short (∼22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe novel microarray platform for genome-wide profiling mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties LNA were exploited to design probe sets uniform, high-affinity hybridizations yielding highly accurate signals able discriminate between single nucleotide differences and, hence, closely related...
Systemic iron homeostasis is mainly controlled by the liver through synthesis of peptide hormone hepcidin (encoded Hamp), key regulator duodenal absorption and macrophage release. Here we show that liver-specific microRNA miR-122 important for regulating Hamp mRNA expression tissue levels. Efficient specific depletion injection a locked-nucleic-acid-modified (LNA-modified) anti-miR into WT mice caused systemic deficiency, characterized reduced plasma levels, mildly impaired hematopoiesis,...
It is currently not well known how necroptosis and responses manifest in vivo. Here, we uncovered a molecular switch facilitating reprogramming between two alternative modes of signaling hepatocytes, fundamentally affecting immune hepatocarcinogenesis. Concomitant necrosome NF-κB activation which physiologically express low concentrations receptor-interacting kinase 3 (RIPK3), did lead to immediate cell death but forced them into prolonged "sublethal" state with leaky membranes, functioning...
The gamma decay from Coulomb excitation of 68Ni at 600 MeV/nucleon on a Au target was measured using the RISING setup fragment separator GSI. beam produced by fragmentation reaction 86Kr 900 9Be and selected separator. rays were with HPGe detectors forward angles BaF2 scintillators backward angles. spectra show peak centered approximately 11 MeV, whose intensity can be explained in terms an enhanced strength dipole response function (pygmy resonance). Such pygmy structure has been predicted...
Mice with a cardiomyocyte (CM)-restricted knockout of signal transducer and activator transcription 3 (STAT3-KO) develop spontaneous heart failure. We investigated the impact STAT3-mediated regulation microRNAs for pathophysiological alterations in heart. MicroRNAchip qRT–PCR analysis revealed elevated cardiac expression miR-199a STAT3-KO mice. Lentiviral shRNA-mediated STAT3-knock-down neonatal rat CMs markedly increased promoter activity levels indicative suppressive effect STAT3 on...
Abstract Background MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression and their is frequently altered in human diseases, including cancer. To correlate clinically relevant parameters with microRNA expression, total RNA prepared from samples were archived for various time periods frozen tissue banks but, unfortunately, integrity not always preserved these tissues. Here, we investigate whether experimentally induced degradation affects profiles. Results...
Abstract Long‐term culture of human mesenchymal stromal cells (MSC) has implications on their proliferation and differentiation potential we have demonstrated that this is associated with up‐regulation the five microRNAs miR‐29c, miR‐369‐5p, miR‐371, miR‐499, let‐7f. In study, examined role these senescence‐associated for cellular aging MSC. Proliferation was reduced upon transfection miR‐499. Adipogenic impaired by miR‐369‐5p whereas it highly increased miR‐371. This accompanied respective...
MYCN is a master regulator controlling many processes necessary for tumor cell survival. Here, we unravel microRNA network that causes suppressive effects in MYCN-amplified neuroblastoma cells. In profiling studies, histone deacetylase (HDAC) inhibitor treatment most strongly induced miR-183. Enforced miR-183 expression triggered apoptosis, and inhibited anchorage-independent colony formation vitro xenograft growth mice. Furthermore, the mechanism of induction was found to contribute death...
microRNAs (miRNAs) are short RNAs that regulate gene expression in various processes, including immune response. Altered response is a pivotal event the pathogenesis of celiac disease (CD), and miRNAs could have role modulating both innate adaptive to gluten patients.We compared miRNA profiles duodenal biopsies controls CD patients by array. Differentially expressed were validated controls, Marsh 3A-B, 3C quantitative PCR (qPCR). Target was assessed qPCR, western blotting,...
We present new $\ensuremath{\Sigma}$ beam asymmetry data for $\ensuremath{\eta}$ meson photoproduction on the proton, using a novel tagged, laser backscattered, linearly polarized photon up to 1.1 GeV. The show large, positive asymmetries, at all incident energies. In addition ${S}_{11}(1535)$ and ${D}_{13}(1520)$ resonances necessary reproduce cross sections, ${P}_{13}(1720)$ ${D}_{15}(1675)$ ``four stars'' contribute observable, but cannot strong forward asymmetries measured energies...
Abstract microRNAs are an abundant class of small non-coding RNAs that control gene expression post-transcriptionally. Importantly, microRNA activity participates in the regulation cellular processes and is a potentially valuable source biomarkers diagnosis prognosis human diseases. Here we introduce miQPCR, innovative method to quantify by using Real-Time PCR. miQPCR exploits T4 RNA ligase activities extend uniformly microRNAs’ 3′-ends addition linker-adapter. The adapter then used as...