A5048325818- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Barrier Structure and Function Studies
- interferon and immune responses
- Caveolin-1 and cellular processes
- Ion Transport and Channel Regulation
- RNA modifications and cancer
- Mathematical Biology Tumor Growth
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Galectins and Cancer Biology
- Circadian rhythm and melatonin
- Axon Guidance and Neuronal Signaling
- Dietary Effects on Health
- Celiac Disease Research and Management
- Viral gastroenteritis research and epidemiology
- Evolution and Genetic Dynamics
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Cancer, Stress, Anesthesia, and Immune Response
IFOM
2022-2024
University of Milano-Bicocca
2014
Abstract The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from solid-like to liquid-like state, occurs various tissues. Whether this tissue-level mechanical impacts phenotypes during carcinoma progression remains unclear. Here we report that the large fluctuations cell density accompany unjamming result repeated deformations of cells and nuclei. This triggers cellular mechano-protective...
Immunotherapy is improving the prognosis and survival of cancer patients, but despite encouraging outcomes in different cancers, majority tumors are resistant to it, immunotherapy combinations often accompanied by severe side effects. Here, we show that a periodic fasting-mimicking diet (FMD) can act on tumor microenvironment increase efficacy (anti-PD-L1 anti-OX40) against poorly immunogenic triple-negative breast (TNBCs) expanding early exhausted effector T cells, switching metabolism from...
microRNAs (miRNAs) are short RNAs that regulate gene expression in various processes, including immune response. Altered response is a pivotal event the pathogenesis of celiac disease (CD), and miRNAs could have role modulating both innate adaptive to gluten patients.We compared miRNA profiles duodenal biopsies controls CD patients by array. Differentially expressed were validated controls, Marsh 3A-B, 3C quantitative PCR (qPCR). Target was assessed qPCR, western blotting,...
Abstract Circulating tumor cell (CTC) clusters are associated with increased metastatic potential and worse patient prognosis, but rare, difficult to count, poorly characterized biophysically. The PillarX device described here is a bimodular microfluidic (Pillar‐device an X‐magnetic device) profile single CTCs from whole blood based on their size, deformability, epithelial marker expression. Larger, less deformable large cells captured in the Pillar‐device sorted according pillar gap sizes....
<title>Abstract</title> Tissue-level phase transitions are emerging as a crucial mechanism in tumour development and metastasis. This study aims to identify molecular determinants physical conditions that control active wetting solid-to-fluid transition of epithelial tissues. We focused on IRSp53, protein linking plasma membranes the cytoskeleton. Depleting MCF10 DCIS.com cells, disrupts coordinated collective movement by promoting local fluctuations cell velocity resulting increased tissue...
Cancers feature substantial intratumoral heterogeneity of genetic and phenotypically distinct lineages. Although interactions between coexisting lineages are emerging as a potential contributor to tumor evolution, the extent nature these remain largely unknown. We postulated that tumors develop ecological sustain diversity facilitate metastasis. Using combination fluorescent barcoding, mathematical modeling, metabolic analysis, in vivo models, we show Allee effect, i.e., growth dependency on...
The blood-brain barrier (BBB) acquires unique properties to regulate neuronal function during development. formation of the BBB, which occurs in tandem with angiogenesis, is directed by Wnt/β-catenin signaling pathway. Yet exact molecular interplay remains elusive. Our study reveals G protein–coupled receptor GPR126 as a critical target canonical Wnt signaling, essential for development BBB's distinctive vascular characteristics and its functional integrity. Endothelial cell–specific...
Abstract The blood–brain barrier (BBB) acquires unique properties for regulation of the neuronal function during development. genesis BBB coupled with angiogenesis is orchestrated by Wnt/β-catenin signaling pathway. Aside from importance signaling, molecular mechanisms that regulate these processes are poorly understood. Here, we identify brain endothelial adhesion G-protein–coupled receptor Gpr126 as a novel target gene required postnatal development, and its expression detrimental ischemic...