A5086574735- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Lipid metabolism and biosynthesis
- Diet, Metabolism, and Disease
- Alcohol Consumption and Health Effects
- Advanced Glycation End Products research
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatitis C virus research
- Hepatocellular Carcinoma Treatment and Prognosis
- Birth, Development, and Health
- Heme Oxygenase-1 and Carbon Monoxide
- Microbial Metabolic Engineering and Bioproduction
- Gut microbiota and health
- Genetic and phenotypic traits in livestock
- Lipid metabolism and disorders
- Pancreatic function and diabetes
- S100 Proteins and Annexins
- Colorectal Cancer Screening and Detection
- Folate and B Vitamins Research
- Hormonal and reproductive studies
Brooklyn College
2020-2023
City University of New York
2022-2023
Columbia University
2011-2022
Edinburgh Royal Infirmary
2013
Inova Health System
2013
Rutgers, The State University of New Jersey
2007-2011
University of North Carolina at Chapel Hill
2004-2005
Increased translocation of intestinal bacteria is a hallmark chronic liver disease and contributes to hepatic inflammation fibrosis. Here we tested the hypothesis that microbiota Toll-like receptors (TLRs) promote hepatocellular carcinoma (HCC), long-term consequence injury, inflammation, Hepatocarcinogenesis in chronically injured livers depended on TLR4 activation non-bone-marrow-derived resident cells. were not required for HCC initiation but promotion, mediating increased proliferation,...
In contrast to microbially triggered inflammation, mechanisms promoting sterile inflammation remain poorly understood. Damage-associated molecular patterns (DAMPs) are considered key inducers of following cell death, but the relative contribution specific DAMPs, including high-mobility group box 1 (HMGB1), is ill defined. Due postnatal lethality Hmgb1-knockout mice, role HMGB1 in and disease processes vivo remains controversial. Here, using conditional ablation strategies, we have...
Objective Chemokines are known to play an important role in the pathophysiology of alcoholic hepatitis (AH), a form acute-on-chronic liver injury frequently mediated by gut derived lipopolysaccharide (LPS). In our study, we hypothesise that chemokine CCL20, one most upregulated chemokines patients with AH, is implicated pathogenesis AH mediating LPS induced injury. Design CCL20 gene expression and serum levels their correlation disease severity were assessed AH. Cellular sources its...
The metabolism of long-chain fatty acids in brain and their incorporation into signaling molecules such as diacylglycerol LPA structural components membranes, including myelin, requires activation by acyl-CoA synthetase (ACSL). Because ACSL3 ACSL6 are the predominant ACSL isoforms brain, we cloned characterized these from rat identified a novel clone (ACSL6_v2). ACSL6_v2 previously reported ACSL6_v1 represent splice variants that include exon 13 or 14, respectively. Homologue sequences both...
Although short-term incubation of hepatocytes with oleic acid (OA) stimulates secretion apolipoprotein B100 (apoB100), exposure to higher doses OA for longer periods inhibits in association induction endoplasmic reticulum (ER) stress. Palmitic (PA) induces ER stress, but its effects on apoB100 are unclear. Docosahexaenoic (DHA) secretion, stress have not been studied. We compared the each these fatty acids and McArdle RH7777 (McA) cells: PA induced inhibited at doses; was more potent because...
Fibrosis and cancer represent two major complications of chronic liver disease. MicroRNAs have been implicated in the development fibrosis cancer, thus constituting potential therapeutic targets. Here, we investigated role microRNA‐21 (miR‐21), a microRNA that has multiple organs also suggested to act as an “oncomir.” Accordingly, miR‐21 was showed strongest up‐regulation activated hepatic stellate cells (HSCs) models fibrogenesis, with 8‐fold 24‐fold induction compared quiescent HSCs....
Mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome (CDS), characterized by excessive storage of triacylglycerol tissues. CGI-58 is an alpha/beta-hydrolase fold enzyme expressed all vertebrates. The carboxyl terminus includes a highly conserved consensus sequence (HXXXXD) for acyltransferase activity. Mouse was Escherichia coli as fusion protein with two amino terminal 6-histidine tags. Recombinant displayed acyl-CoA-dependent activity to lysophosphatidic acid, but not other...
Maternal obesity during pregnancy adversely impacts offspring health, predisposing them to chronic metabolic diseases characterized by insulin resistance, dysregulated macronutrient metabolism, and lipid overload, such as metabolic-associated fatty liver disease (MAFLD). Choline is a semi-essential nutrient involved in one-carbon metabolism that compromised MAFLD progression. Here, we investigated under high-fat (HF) obesogenic feeding how maternal choline supplementation (CS) influenced the...
Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the of prenatal epigenome. In this study, we examined maternal choline (CS), a donor, interacts with and HF feeding to alter global site-specific methylation in offspring. We fed wild-type C57BL/6J mouse dams diet or without CS throughout gestation. After weaning, were...
Adipose triglyceride lipase (ATGL) catalyzes the first step of triacylglycerol hydrolysis in adipocytes. Abhydrolase domain 5 (ABHD5) increases ATGL activity by an unknown mechanism. Prior studies have suggested that expression ABHD5 is limiting for lipolysis adipocytes, as addition recombinant vitro TAG hydrolase adipocyte lysates. To test this hypothesis vivo, we generated transgenic mice express 6-fold higher adipose tissue relative to wild-type (WT) mice. In vivo increased a similar...
Nonalcoholic fatty liver disease (NAFLD) is the most common in United States and world; with no Food Drug Administration-approved pharmacological treatment available, it remains an area of unmet medical need. In nonalcoholic steatohepatitis (NASH), important predictor clinical outcome fibrosis stage. Moreover, Administration recommends that trials for drugs to treat this include patients stage 2 or greater. Therefore, when using animal models investigating pathophysiology NAFLD preclinical...
Long chain fatty acids are converted to acyl-CoAs by acyl-CoA synthetase (fatty acid CoA ligase: AMP forming, E.C. 6.2.1.3; ACS). Escherichia coli has a single ACS, FadD, that is essential for growth when the sole carbon and energy source. Rodents have five ACS isoforms differ in substrate specificity, tissue expression, subcellular localization believed channel toward distinct metabolic pathways. We expressed rat 1–5 an E. strain lacked FadD. All were fadD or fadDfadR had specific...