A5011790505- Enzyme Structure and Function
- Lipid metabolism and biosynthesis
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Metal-Catalyzed Oxygenation Mechanisms
- Enzyme Catalysis and Immobilization
- Research in Cotton Cultivation
- Microbial metabolism and enzyme function
- Diabetes Management and Research
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Neuropeptides and Animal Physiology
- Erythrocyte Function and Pathophysiology
- Protein Hydrolysis and Bioactive Peptides
- RNA Research and Splicing
- Advancements in Transdermal Drug Delivery
- Metalloenzymes and iron-sulfur proteins
- Metabolism, Diabetes, and Cancer
- Genetically Modified Organisms Research
- Nuclear Structure and Function
- Cholesterol and Lipid Metabolism
- Research on Leishmaniasis Studies
- Parasitic Infections and Diagnostics
- Oral microbiology and periodontitis research
Autonomous University of San Luis Potosí
2015-2025
Instituto Politécnico Nacional
2012-2017
Center for Research and Advanced Studies of the National Polytechnic Institute
2015-2017
Institute for Scientific and Technological Research
2012-2015
Rutgers, The State University of New Jersey
2008-2014
Universidad Nacional Autónoma de México
1998-2009
Michigan State University
2007
Université Paris-Sud
2005-2007
John Innes Centre
2007
Centre National de la Recherche Scientifique
2001-2007
CGI-58/ABHD5 coactivates adipose triglyceride lipase (ATGL). In adipocytes, CGI-58 binds to perilipin 1A on lipid droplets under basal conditions, preventing interaction with ATGL. Upon activation of protein kinase A (PKA), is phosphorylated and rapidly disperses into the cytoplasm, enabling coactivation. Because amino acid sequence murine has a predicted PKA consensus RKYS(239)S(240), we hypothesized that phosphorylation involved in this process. We show Ser239 substrate for using...
Mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome (CDS), characterized by excessive storage of triacylglycerol tissues. CGI-58 is an alpha/beta-hydrolase fold enzyme expressed all vertebrates. The carboxyl terminus includes a highly conserved consensus sequence (HXXXXD) for acyltransferase activity. Mouse was Escherichia coli as fusion protein with two amino terminal 6-histidine tags. Recombinant displayed acyl-CoA-dependent activity to lysophosphatidic acid, but not other...
Canary grass is used as traditional food for diabetes and hypertension treatment. The aim of this work to characterize the biological activity encrypted peptides released after gastrointestinal digestion canary seed proteins. showed 43.5% inhibition dipeptidyl peptidase IV (DPPIV) 73.5% angiotensin-converting enzyme (ACE) activity. An isolated perfused rat heart system was evaluate vasoactive effect. Nitric oxide (NO), a major vasodilator agent, evaluated in venous effluent from heart. (1...
Summary The xanthine oxidases and dehydrogenases are among the most conserved enzymes in all living kingdoms. They contain molybdopterin cofactor Moco. We show here that fungi, addition to dehydrogenase, a completely different enzyme is able catalyse oxidation of uric acid. In Aspergillus nidulans this coded by xanA gene. have cloned gene determined its sequence. A deletion has same phenotype as previously known xanA1 miss‐sense mutation. Homologues exist only fungal kingdom. inactivated...
His6-tagged xanthine/α-ketoglutarate (αKG) dioxygenase (XanA) of Aspergillus nidulans was purified from both the fungal mycelium and recombinant Escherichia coli cells, properties two forms protein were compared. Evidence obtained for N- O-linked glycosylation on fungus-derived XanA, which aggregates into an apparent dodecamer, while bacterium-derived XanA is free behaves as a monomer. Immunological methods identify phosphothreonine in with phosphoserine also detected protein. Mass...
SUMMARY The glycolytic enzyme triosephosphate isomerase catalyses the isomerization between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Here we report that Trichomonas vaginalis contains 2 fully functional tpi genes. Both genes are located in separated chromosomal context with different promoter regulatory elements encode ORFs of 254 amino acids; only differences them character 4 acids α -helices 1, 8. Semi-quantitative RT-PCR assays showed tpi2 transcript is approximately...
Current sequence-based approaches to identify enzyme functional shifts, such as promiscuity, have proven be highly dependent on a priori knowledge, hampering our ability reconstruct evolutionary history behind these mechanisms. Hidden Markov Model (HMM) profiles, broadly used classify families, can useful distinguish between closely related families with different specificities. The (βα)8-isomerase HisA/PriA family, involved in L-histidine (HisA, mono-substrate) biosynthesis most bacteria...
The active site of glucosamine-6-phosphate deaminase (EC 3.5.99.6, formerly 5.3.1.10) from Escherichia coli was first characterized on the basis crystallographic structure enzyme bound to competitive inhibitor 2-amino-2-deoxy-glucitol 6-phosphate. corresponds R allosteric state enzyme; it shows side-chain His143 in close proximity O5 atom inhibitor. This arrangement suggests that could have a role catalysis ring-opening step glucosamine 6-phosphate whose alpha-anomer is true substrate....
Nanoparticles (NPs) have been widely studied as an alternative to antibiotic use due their antimicrobial properties at lower concentrations. Enterococcus faecalis is a facultative Gram-positive microorganism inhabiting the gastrointestinal tract of humans and animals. It can also be present in other environments such oral cavity, water, sewage, soil food.We evaluated whether E. could develop resistance silver NPs (AgNPs) after exposure sublethal concentrations NPs.Proteomic analyses revealed...
In lipolysis, the activating function of CGI-58 is regulated by its interaction with perilipin 1 (PLIN1) localized on lipid droplet (LD), and release controlled phosphorylation. Once lipolysis stimulated catecholamines, protein kinase A (PKA)-mediated phosphorylation enables dissociation CGI-58/PLIN1 complex, thereby recruiting adipose triglyceride lipase (ATGL) hormone-sensitive (HSL) to initiate fatty acid release. It has been shown that mouse mutant S239E, which mimics this residue, able...
G0S2 is a small protein of 103 residues in length that involved multiple cellular processes. To date, several reports have shown functions by making direct protein–protein interactions with key proteins. In lipolysis, specifically interacts adipose triglyceride lipase, inhibiting its activity and resulting lipolysis being downregulated. similar way, also participates the regulation apoptosis, cell proliferation, oxidative phosphorylation; however, information regarding structural biophysical...
After the surprisingly low number of genes identified in human genome, alternative splicing emerged as a major mechanism to generate protein diversity higher eukaryotes. However, it is still not known if its prevalence along genome evolution has contributed overall functional or simply reflects noise. The (βα)8 barrel TIM one most frequent, versatile, and ancient fold encountered among enzymes. Here, we analyze structural modifications present proteins from product events. We found that 87%...
Abstract The effects of visible light on biological systems have been widely studied. In particular, the alterations blue ocular lens recently attracted much attention. Here, we present a study about produced by green and red two different proteins: β L -crystallin ovalbumin. Based differential scanning calorimetry (DSC), circular dichroism (CD), dynamic scattering (DLS) fluorescence emission measurements, found that both wavelengths induce structural changes in these proteins. We also...
The involvement of tyrosine residues in the allosteric function enzyme glucosamine 6-phosphate deaminase from Escherichia coli was first proposed on basis a theoretical analysis sequence and demonstrated by spectrophotometric experiments. Two residues, Tyr121 Tyr254, were indicated as involved mechanism cooperativity regulation [Altamirano et al. (1994) Eur. J. Biochem. 220, 409−413]. replacement threonine or tryptophan altered symmetric character T → R transition (1995) Biochemistry 34,...
The remarkable properties of garlic A. sativum L. have been described, but little is known about Snow mountain garlic. Understanding general aspects this composition, including the presence phenolics, will establish its possible use for health or infer which compounds can contribute to improving it. This study aimed determine ash content, lipid profile, and characterization phenolics in organic content was obtained by common techniques (oven drying, calcination, Kjeldahl method, etc.)....