A5036424793- Gut microbiota and health
- Escherichia coli research studies
- Inflammatory Bowel Disease
- Microscopic Colitis
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Bacterial Genetics and Biotechnology
- Radioactive element chemistry and processing
- RNA and protein synthesis mechanisms
- Diet and metabolism studies
- Genomics and Phylogenetic Studies
- Boron Compounds in Chemistry
- Microbial Community Ecology and Physiology
- Salmonella and Campylobacter epidemiology
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Probiotics and Fermented Foods
- Adipokines, Inflammation, and Metabolic Diseases
- Apelin-related biomedical research
University of Girona
2015-2024
Universidad de Granada
2022
Hospital Universitari de Girona Doctor Josep Trueta
2020
Hospital Santa Caterina
2020
University of Aberdeen
2020
Microbe, Intestine, Inflammation and Host Susceptibility
2013-2019
Inserm
2013-2016
Université Clermont Auvergne
2008-2016
University College London
2007
Institute of Immunology
2007
<h3>Objective</h3> Western diet is a risk factor for Crohn9s disease (CD). Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) abnormally expressed in CD patients. This allows adherent-invasive <i>Escherichia coli</i> (AIEC) to colonise the gut mucosa and leads inflammation. We assessed effects of high fat/high sugar (HF/HS) on microbiota composition, barrier integrity susceptibility infection transgenic CEABAC10 mice expressing human CEACAMs. <h3>Design</h3> Colonic...
Abstract Recent advances have shown that the abnormal inflammatory response observed in CD involves an interplay among intestinal microbiota, host genetics and environmental factors. The escalating consumption of fat sugar Western countries parallels increased incidence during latter 20 th century. impact a HF/HS diet mice was evaluated for gut micro-inflammation, microbiota composition, function selection E. coli population. created specific environment gut, correlated with mucosa dysbiosis...
Escherichia coli, particularly the adherent-invasive E. coli (AIEC) pathovar, has been increasingly implicated in ethiopathogenesis of Crohn's disease (CD). We describe richness, abundance, diversity, and pathogenic features AIEC strains that colonize intestinal mucosa. Approximately 100 colonies per biopsy from 20 CD patients (18 biopsies colon 23 ileum) 28 healthy controls (C) (25, colon; 27, were isolated. Repetitive extragenic palindrome-polymerase chain reaction (Rep-PCR) pulsed field...
Akkermansia muciniphila and Faecalibacterium prausnitzii, cohabitants in the intestinal mucosa, are considered members of a healthy microbiota reduction both species occurs several disorders, including inflammatory bowel disease. Little is known however about possible link between quantity these species, which circumstances this may occur. This study aims to determine abundances co-occurrence two order elucidate conditions that compromise their presence gut. Loads A. muciniphila, total F....
Bacteria might play a role in the pathogenesis of Crohn's disease (CD), and patients harbor different type density gut microbiota compared with normal healthy subjects. Thus, aim this study was to compare adhered mucosa CD that subjects.Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) 16S rRNA gene fragments used identify dominant bacterial species present fresh biopsy samples obtained from 15 19 Two suffering ulcerative colitis 1 ischemic also were...
Adherent-invasive Escherichia coli (AIEC) are abnormally predominant on Crohn's disease (CD) ileal mucosa. AIEC reference strain LF82 adheres to enterocytes via the common type 1 pili adhesin FimH and recognizes CEACAM6 receptors expressed CD epithelial cells. The fimH genes of 45 47 non-AIEC strains were sequenced. phylogenetic tree based DNA sequences indicated that predominantly express with amino acid mutations a recent evolutionary origin - typical signature pathoadaptive changes...
Nutrition therapy is the cornerstone of treating diabetes mellitus. The inclusion fish (particularly oily fish) at least two times per week recommended by current international dietary guidelines for type 2 diabetes. In contrast to a large number human studies examining effects on different cardiovascular risk factors, little research this topic available in patients with aims pilot study were investigate sardine-enriched diet metabolic control, adiponectin, inflammatory markers, erythrocyte...
Faecalibacterium prausnitzii comprises 2 phylogroups, whose abundance in healthy and diseased gut conjunction with Escherichia coli has not yet been studied. This work aims to determine the contribution of F. phylogroups I II intestinal disease assess their potential diagnostic usefulness as biomarkers for diseases.Total prausnitzii, its E. loads were determined by quantitative polymerase chain reaction targeting 16S rRNA gene on biopsies from 31 controls (H), 45 patients Crohn's (CD), 25...
Abstract Gut microbial and metabolite alterations have been linked to the pathogenesis of inflammatory bowel diseases. Here we perform a multi-omics microbiome analysis longitudinal cohort Crohn’s disease patients undergoing autologous hematopoietic stem cell transplantation, investigational therapy that induces drug free remission in subset patients. Via comparison who responded maintained remission, but experienced relapse did not respond therapy, identify shared functional signatures...
Abstract Background Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation CD. Because bacterial biofilms gut mucosa are suspected to play role CD biofilm formation feature certain pathogenic E. strains, we compared capacity 27 AIEC 38 non-AIEC strains isolated from intestinal mucosa. Biofilm was then contrasted with phenotype, serotype, phylotype,...
ABSTRACT Adherent-invasive Escherichia coli (AIEC) pathovar strains, which are associated with Crohn's disease, share many genetic and phenotypic features extraintestinal pathogenic E. (ExPEC) but little is known about the level of similarity between two pathovars. We aimed to determine frequency strains “AIEC phenotype” among a collection ExPEC further search for common phylogenetic origin intestinal AIEC strains. The adhesion, invasion, intramacrophage replication capabilities (AIEC...
Faecalibacterium prausnitzii depletion in intestinal diseases has been extensively reported, but little is known about intraspecies variability. This work aims to determine if subjects with gastrointestinal disease host mucosa-associated F. populations different from those hosted by healthy individuals. A new species-specific PCR-denaturing gradient gel electrophoresis (PCR-DGGE) method targeting the 16S rRNA gene was developed fingerprint biopsy specimens 31 control (H) and 36 Crohn's (CD),...
The composition of the intestinal microbiota is altered in Crohn's disease [CD] patients. objective this study was to evaluate qualitative and quantitative changes CD patients 3 months treatment with adalimumab [ADA], determine whether or not these are produced towards recovery normal, healthy-like microbiota. composition, Faecalibacterium prausnitzii / Escherichia coli relationship as dysbiosis indicator, were studied at baseline [T0], one month [T1], [T3] after starting using a polymerase...
Abstract Gut microbiota plays an important role in the regulation of immune system and host’s metabolism. We aimed to characterize gut Tunisian participants with without diabetes. enrolled ten type 1 diabetes mellitus (T1DM), patients 2 (T2DM), 11 subjects Bacteria was quantified fecal samples by quantitative PCR (qPCR). Statistical tests multivariate analysis were performed using RStudio program. Results showed that proportions Firmicutes, Akkermansia muciniphila, Faecalibacterium...
Abstract Background An Escherichia coli ( E. ) pathotype with invasive properties, first reported by Darfeuille-Michaud and termed adherent-invasive (AIEC), was shown to be prevalent in up half the individuals Crohn’s Disease (CD), suggesting that these bacteria could involved pathophysiology of CD. Among genes related AIEC pathogenicity, fim has potential generate an inflammatory reaction from intestinal epithelial cells macrophages, as it interacts TLR4, inducing production cytokines...
Background &amp; Aims Adherent-invasive E. coli (AIEC) has largely been implicated in the pathogenesis of Crohn’s disease (CD). strains with similar genetic backgrounds and virulence genes profiles have associated other intestinal disorders, such as ulcerative colitis (UC), colorectal cancer (CRC), coeliac (CeD), but role AIEC these diseases remains unexplored. We aimed to assess distribution, abundance, pathogenic features UC, CRC, CeD. Methods The phenotype was investigated 4,233...
Adherent-invasive Escherichia coli (AIEC) have been involved in Crohn's disease (CD). Currently, AIEC are identified by time-consuming techniques based on vitro infection of cell lines to determine their ability adhere and invade intestinal epithelial cells as well survive replicate within macrophages. Our aim was find signature sequences that can be used identify the pathotype. Comparative genomics performed between three E. strain pairs, each pair comprised one non-AIEC with identical...
The Na[3,3'-Fe(8-I-1,2-C2B9H10)2] and Na[2,2'-M(1,7-C2B9H11)] (M = Co3+, Fe3+) small molecules are synthesized the X-ray structures of [(H3O)(H2O)5][2,2'-Co(1,7-C2B9H11)2] [Cs(MeCN)][8,8'-I2-Fe(1,2 C2B9H10)2], both displaying a transoid conformation [M(C2B9)2]- framework, reported. Importantly, supramolecular structure presents 2D layers leading to lamellar arrangement anions while cation form polymeric water rings made six- four-membered connected via OH⋯H hydrogen bonds; B-H⋯O contacts...
To date no molecular tools are available to identify the adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease and colonizes intestine of different hosts. Current techniques based on phenotypic screening isolates extremely time-consuming. The aim this work was search for signature traits assist in rapid AIEC identification. occurrence at least 54 virulence genes (VGs), resistance 30 antibiotics distribution FimH ChiA amino acid substitutions...
BackgroundPrediabetes and old age are both high risk factors for developing Type 2 Diabetes (T2D), while obesity is one of the most important triggering disease. Nutritional interventions effective tool preventing T2D, as they improve different biochemical anthropometric outcomes growth-promoting/inhibiting gut microbiota populations. However, to date there no specific dietary recommendations stop development T2D in elderly groups, whom hypocaloric diets other commonly used weight-loss...