A5048704706- Escherichia coli research studies
- Inflammatory Bowel Disease
- Gut microbiota and health
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Clostridium difficile and Clostridium perfringens research
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Drug Transport and Resistance Mechanisms
- Chemical Synthesis and Analysis
- Mycobacterium research and diagnosis
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Enterobacteriaceae and Cronobacter Research
- Pharmacological Effects of Natural Compounds
- Macrophage Migration Inhibitory Factor
- Glycosylation and Glycoproteins Research
- Eosinophilic Esophagitis
- Agriculture and Rural Development Research
- African Botany and Ecology Studies
- Immunodeficiency and Autoimmune Disorders
- Legume Nitrogen Fixing Symbiosis
Microbe, Intestine, Inflammation and Host Susceptibility
2016-2025
Inserm
2015-2025
Université Clermont Auvergne
2011-2025
Clermont Université
2009-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Instituts Universitaires de Technologie
2009-2022
Centre de Recherche en Nutrition Humaine d'Auvergne
2022
Génétique, Diversité, Écophysiologie des Céréales
2013-2015
Abnormal expression of CEACAM6 is observed at the apical surface ileal epithelium in Crohn's disease (CD) patients, and CD lesions are colonized by pathogenic adherent-invasive Escherichia coli (AIEC). We investigated ability AIEC reference strain LF82 to colonize intestinal mucosa induce inflammation CEABAC10 transgenic mice expressing human CEACAMs. virulent bacteria, but not nonpathogenic E. K-12, were able persist gut severe colitis with reduced survival rate, marked weight loss,...
Adherent invasive Escherichia coli [AIEC] are abnormally predominant on the ileal mucosa of Crohn's disease [CD] patients. They bind to CEACAM6 receptor expressed surface epithelial cells. We aimed assess potential bacteriophages, viruses infecting bacteria, decrease levels AIEC bacteria associated with intestinal mucosa.We combined ex vivo and in experiments murine human samples quantify ability virulent bacteriophages target prototype strain LF82.We found that three were able replicate...
Abnormal expression of CEACAM6 observed on the ileal epithelium in Crohn's disease (CD) patients allows adherent-invasive Escherichia coli (AIEC) to colonize gut mucosa. Since intestinal permeability is significantly increased CD patients, we aimed at investigating whether and how AIEC alter barrier function.Tissue microarray was performed biopsies from quiescent active phases. CEABAC10 or wildtype mice were orally challenged with 10(9) bacteria. Intestinal assessed by measuring 4 kDa...
Adherent-invasive Escherichia coli (AIEC) have previously been shown to induce gut inflammation in patients with Crohn's disease (CD). We developed a set of mannosides prevent AIEC attachment the by blocking FimH bacterial adhesin. The crystal structure lectin domain complex lead thiazolylaminomannoside highlighted preferential position for pharmacomodulations. A small library analogues showing nanomolar affinity was then developed. Notably, intestinal cells efficiently prevented most active...
BackgroundAdherent-invasive Escherichia coli (AIEC), which colonize the ileal mucosa of patients with Crohn's disease (CD), are able to adhere and invade intestinal epithelial cells. Overexpression glycoprotein CEACAM6 on host cells favors AIEC attachment inflammation. We investigated ability Saccharomyces cerevisiae CNCM I-3856 inhibit adhesion reduce colitis.
Determinants of urosepsis in Escherichia coli remain incompletely defined. Cyclomodulins (CMs) are a growing functional family toxins that hijack the eukaryotic cell cycle. Four cyclomodulin types actually known E. coli: cytotoxic necrotizing factors (CNFs), cycle-inhibiting factor (Cif), cytolethal distending (CDTs), and pks-encoded toxin. In present study, distribution CM-encoding genes functionality these were investigated 197 strains isolated from patients with community-acquired (n =...
ABSTRACT The ileal lesions of Crohn's disease (CD) patients are colonized by adherent-invasive Escherichia coli (AIEC) bacteria. These bacteria adhere to mannose residues expressed CEACAM6 on host cells in a type 1 pilus-dependent manner. In this study, we investigated different antagonists FimH, the adhesin pili, for their ability block AIEC adhesion intestinal epithelial (IEC). Monovalent and multivalent derivatives n -heptyl α- d- mannoside (HM), nanomolar antagonist were tested vitro IEC...
n-Heptyl α-d-mannose (HM) is a nanomolar antagonist of FimH, virulence factor E. coli. Herein we report on the construction multivalent HM-based glycopolymers as potent antiadhesives type 1 piliated We investigate glycopolymer/FimH and glycopolymer/bacteria interactions show that efficiently inhibit bacterial adhesion disrupt established cell-bacteria in vitro at very low concentration (0.1 μM mannose unit basis). On valency-corrected basis, are, respectively, 10(2) 10(6) times more than HM...
Abstract Blocking the adherence of bacteria to cells is an attractive complementary approach current antibiotic treatments, which are faced with increasing resistance. This strategy has been particularly studied in context urinary tract infections (UTIs), adhesion pathogenic Escherichia coli strains uroepithelial prevented by blocking FimH adhesin expressed at tips organelles called fimbriae. Recently, we extended antiadhesive concept, showing that potent antagonists can block attachment...
Ulcerative colitis (UC) is a chronic inflammatory condition linked to intestinal microbial dysbiosis, including the expansion of E. coli strains related extra-intestinal pathogenic coli. These "pathobionts" exhibit properties, but their potential promote UC unclear due lack relevant animal models. Here, we established mouse model using representative pathobiont strain (p19A), and mice lacking single immunoglobulin toll-interleukin 1 receptor domain (SIGIRR), deficiency increasing...
The Escherichia coli surfaceome consists mainly of the large surface organelles expressed by organism to navigate and interact with surrounding environment. current study focuses on type I fimbriae flagella. These polymeric are composed hundreds thousands subunits, their size often preventing them from being studied in native form. Recent studies accumulating which demonstrate glycosylation proteins or virulence factors pathogens, including E. . Using biochemical glycobiological techniques,...
The chemical stability of potent<italic>E. coli</italic>anti-adhesives was improved by substitution the anomeric nitrogen short linkers.
Abstract Background An Escherichia coli ( E. ) pathotype with invasive properties, first reported by Darfeuille-Michaud and termed adherent-invasive (AIEC), was shown to be prevalent in up half the individuals Crohn’s Disease (CD), suggesting that these bacteria could involved pathophysiology of CD. Among genes related AIEC pathogenicity, fim has potential generate an inflammatory reaction from intestinal epithelial cells macrophages, as it interacts TLR4, inducing production cytokines...
The intestinal mucus layer has a dual role in human health constituting well-known microbial niche that supports gut microbiota maintenance but also acting as physical barrier against enteric pathogens. Enterotoxigenic Escherichia coli (ETEC), the major agent responsible for traveler's diarrhea, is able to bind and degrade mucins, representing an important understudied virulent trait of pathogen. Using set complementary vitro approaches simulating digestive environment, this study aimed...
<italic>E. coli</italic>antiadhesives were synthesized and assessed against<italic>E. coli</italic>strains isolated from patients with cystitis, osteoarticular infections or Crohn's disease.
A new versatile cyclic polymer platform for the design of advanced materials was prepared by combining ring-expansion metathesis polymerization (REMP) and click chemistry. Cyclic poly(norbornenyl azlactone) backbones were synthesized over an unprecedented length range with number-average degree (DPn) ranging from 25 to 1000. The topology thoroughly characterized using 1H NMR, size exclusion chromatography (SEC) multiangle light scattering (MALS) viscometer detection. Postpolymerization...
ABSTRACT Adherent-invasive Escherichia coli (AIEC) were investigated for their involvement in the induction/chronicity of intestinal inflammation Crohn's disease (CD). AIEC gut establishment is favoured by overexpression glycoprotein CEACAM6 ileal epithelium. We generated a transgenic mouse model, named ‘Vill-hCC6’, which human gene was under control villin promoter, conditioning expression small intestine. demonstrated that strongly expressed intestine mucosa and correlated with numerous...
The Western diet, rich in lipids and n-6 polyunsaturated fatty acids (PUFAs), favors gut dysbiosis observed Crohn's disease (CD). aim of this study was to assess the effects rebalancing n-6/n-3 PUFA ratio CEABAC10 transgenic mice that mimic CD. Mice individual cages with running wheels were randomized three diet groups for 12 weeks: high-fat (HFD), HFD + linseed oil (HFD-LS-O) extruded (HFD-LS-E). Then, they orally challenged once Adherent-Invasive Escherichia coli (AIEC) LF82 pathobiont....
<italic>n</italic>-Heptyl α-<sc>d</sc>-mannose-functionalized nanocapsules are prepared by the Shift'N'Go process and post-modified to ensure aggregation efficient removal of bacteria.
A novel mechanism is revealed by which clinical isolates of adherent-invasive Escherichia coli (AIEC) penetrate into the epithelial cell layer, replicate, and establish biofilms in Crohn's disease. AIEC uses FimH fimbrial adhesin to bind oligomannose glycans on surface host cells. Oligomannose exposed early apoptotic cells are preferred binding targets AIEC, so serve as potential entry points for bacteria layer. Thereafter, propagate laterally intercellular spaces. We demonstrate...