A5045185055- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Computational Drug Discovery Methods
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- Polyamine Metabolism and Applications
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Alzheimer's disease research and treatments
- Metabolism and Genetic Disorders
- Carbohydrate Chemistry and Synthesis
- Machine Learning in Bioinformatics
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Fungal and yeast genetics research
- GABA and Rice Research
- Enzyme Structure and Function
- Cellular transport and secretion
- Origins and Evolution of Life
- Mass Spectrometry Techniques and Applications
- Escherichia coli research studies
- Coenzyme Q10 studies and effects
Université Libre de Bruxelles
2011-2025
Unité de Glycobiologie Structurale et Fonctionnelle
2016-2025
Centre National de la Recherche Scientifique
2009-2025
Université de Lille
2016-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2023
Nantes Université
2020
Chimie et Interdisciplinarité, Synthèse, Analyse, Modélisation
2020
Unité en Sciences Biologiques et Biotechnologies de Nantes
2020
Université de Lorraine
2009-2013
University of Bayreuth
2007-2011
Many plasma membrane transporters are downregulated by ubiquitylation, endocytosis, and delivery to the lysosome in response various stimuli. We report here that two amino acid of Saccharomyces cerevisiae, general permease (Gap1) arginine-specific (Can1), undergo ubiquitin-dependent downregulation their substrates this is not due intracellular accumulation transported acids but transport catalysis itself. Following an approach based on structural modeling, mutagenesis, kinetic parameter...
Substrate-transport-elicited endocytosis is a common control mechanism of membrane transporters avoiding excess uptake external compounds, though poorly understood at the molecular level. In yeast, triggered by their ubiquitylation mediated Rsp5 ubiquitin-ligase, recruited α-arrestin-family adaptors. We here report that transport-elicited arginine transporter Can1 promoted transition to an inward-facing state. This conformational change unveils region N-terminal cytosolic tail targeted Art1...
Amino acid uptake in yeast cells is mediated by about 16 plasma membrane permeases, most of which belong to the amino acid-polyamine-organocation (APC) transporter family. These proteins display various substrate specificity ranges. For instance, general permease Gap1 transports all acids, whereas Can1 and Lyp1 catalyze specific arginine lysine, respectively. Although have different narrow specificities, they are close homologs. Here we investigated molecular rules determining H+-driven...
Abstract Blocking the adherence of bacteria to cells is an attractive complementary approach current antibiotic treatments, which are faced with increasing resistance. This strategy has been particularly studied in context urinary tract infections (UTIs), adhesion pathogenic Escherichia coli strains uroepithelial prevented by blocking FimH adhesin expressed at tips organelles called fimbriae. Recently, we extended antiadhesive concept, showing that potent antagonists can block attachment...
The mitochondrial ADP/ATP carrier imports ADP from the cytosol into matrix for its conversion to ATP by synthase and exports out of mitochondrion replenish eukaryotic cell with chemical energy. Here substrate specificity human AAC1 was determined two different approaches. In first protein functionally expressed in Escherichia coli membranes as a fusion maltose binding effect excess unlabeled compounds on uptake [(32)P]-ATP measured. second approach cytoplasmic membrane Lactococcus lactis....
Apolipoprotein E (apoE) binds the amyloid β peptide (Aβ), one of major culprits in Alzheimer's disease development. The formation apoE:Aβ complexes is implicated both Aβ clearance and fibrillization. However, binding interface between apoE poorly defined despite substantial previous research efforts, exact role pathology remains largely elusive. Here, we compared three main isoforms (E2, E3, E4) for their interaction with Aβ1-42 an early stage aggregation at near physiological conditions....
Apolipoprotein E (apoE) is a forefront actor in the transport of lipids and maintenance cholesterol homeostasis, also strongly implicated Alzheimer's disease. Upon lipid-binding apoE adopts conformational state that mediates receptor-induced internalization lipoproteins. Due to its inherent structural dynamics presence lipids, structure biologically active remains so far poorly described. To address this issue, we developed an innovative hybrid method combining experimental data with...
Antagonists of the Escherichia coli type-1 fimbrial adhesin FimH are recognized as attractive alternatives for antibiotic therapies and prophylaxes against acute recurrent bacterial infections. In this study α-d-mannopyranosides O- or C-linked with an alkyl, alkene, alkyne, thioalkyl, amide, sulfonamide were investigated to fit a hydrophobic substituent up two aryl groups within tyrosine gate emerging from mannose-binding pocket FimH. The results summarized into set structure-activity...
The voltage-dependent anion channel (VDAC) forms the major pore in outer mitochondrial membrane. Its high conducting open state features a moderate selectivity. There is some evidence indicating that electrophysiological properties of VDAC vary with salt concentration. Using theoretical approach molecular basis for this concentration dependence was investigated. Molecular dynamics simulations and continuum electrostatic calculations performed on mouse VDAC1 isoform clearly demonstrate...
ABSTRACT The two-component system BvgAS controls the expression of virulence regulon Bordetella pertussis . BvgS is a prototype bacterial sensor kinases with extracytoplasmic Venus flytrap perception domains. Following its transmembrane segment, harbors cytoplasmic Per-Arnt-Sim (PAS) domain and then predicted 2-helix coiled coil that precede dimerization-histidine-phosphotransfer kinase. homologs have similar organization, or they harbor only between Here, we show regulates enzymatic...
The most prevalent diseases manifested by Escherichia coli are acute and recurrent bladder infections chronic inflammatory bowel such as Crohn's disease. E. clinical isolates express the FimH adhesin, which consists of a mannose-specific lectin domain connected via pilin to tip type 1 pili. Although isolated has affinities in nanomolar range for all high-mannosidic glycans, differentiation between these glycans is based on their capacity form predominantly hydrophobic interactions within...
In the exchange of metabolites and ions between mitochondrion cytosol, voltage-dependent anion channel (VDAC) is a key element, as it forms major transport pathway for these compounds through mitochondrial outer membrane. Numerous experimental studies have promoted idea that VDAC acts regulator essential functions. this study, using combination molecular dynamics simulations, free-energy calculations, electrophysiological measurements, we investigated VDAC, with focus on phosphate...
Commensal and pathogenic enteric bacteria have developed several systems to adapt proton leakage into the cytoplasm resulting from extreme acidic conditions. One such system involves arginine uptake followed by export of decarboxylated product agmatine, carried out arginine/agmatine antiporter (AdiC), which thus works as a virtual pump. Here, using classical targeted molecular dynamics, we investigated at atomic level mechanism transport through AdiC E. coli. Overall, our MD simulation data...