A5091596850- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Hemoglobin structure and function
- Legume Nitrogen Fixing Symbiosis
- Groundwater flow and contamination studies
- Plant-Microbe Interactions and Immunity
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Groundwater and Isotope Geochemistry
- Plant Stress Responses and Tolerance
- Electron Spin Resonance Studies
- Hydrocarbon exploration and reservoir analysis
- Protein Structure and Dynamics
- Soil Carbon and Nitrogen Dynamics
- Biofuel production and bioconversion
- Methane Hydrates and Related Phenomena
- Microbial bioremediation and biosurfactants
- Identification and Quantification in Food
- Mycorrhizal Fungi and Plant Interactions
- Erythrocyte Function and Pathophysiology
University of Parma
2013-2023
University of Pavia
1988-2000
University of Milan
2000
Istituto Pasteur
1994
Policlinico Umberto I
1994
Institute of Genetics and Biophysics
1989-1990
ABSTRACT Occurrence of the hsp70 ( dnaK ) gene was investigated in various members domain Archaea comprising both euryarchaeotes and crenarchaeotes hyperthermophilic bacteria Aquifex pyrophilus Thermotoga maritima representing deepest offshoots phylogenetic trees bacterial 16S rRNA sequences. The not detected 8 10 archaea examined but found A. T. , from which it cloned sequenced. Comparative analyses HSP70 amino acid sequences encoded these genes, others databases, showed that (i) accordance...
This study assesses the effects of specific bacterial endophytes on phytoextraction capacity Ni-hyperaccumulator Noccaea caerulescens, spontaneously growing in a serpentine soil environment. Five metal-tolerant had already been selected for their high Ni tolerance (6 mM) and plant growth promoting ability. Here we demonstrate that individual inoculation is ineffective enhancing translocation N. caerulescens soil, except strains Ncr-1 Ncr-8, belonging to Arthrobacter Microbacterium genera,...
In an open field trial on two agricultural soils in NW Italy, the impact of seed-applied biostimulants rhizosphere bacterial community young maize plants was evaluated. The 16S rDNA profiling carried out control and treated plant samples collected at 4-leaf stage bulk soil. both soils, rhizospheres were significantly enriched Proteobacteria, Actinobacteria, Bacteriodetes, while abundances Acidobacteria, Cloroflexi Gemmatimonadetes decreased compared with Among culturable bacteria genera that...
The gene glnA encoding glutamine synthetase I (GSI) from the archaeum Pyrococcus woesei was cloned and sequenced with Sulfolobus solfataricus as probe. An operon reading frame of 448 amino acids identified within a DNA segment 1,528 bp. encoded protein 49% identical GSI Methanococcus voltae exhibited conserved regions characteristic family. P. aligned available homologs other archaea (S. solfataricus, M. voltae) representative sequences cyanobacteria, proteobacteria, gram-positive bacteria....
The synthetic complexes protohemin-6(7)-L-arginyl-L-alanine (HM-RA) and protohemin-6(7)-L-histidine methyl ester (HM-H) were prepared by condensation of suitably protected Arg-Ala or His residues with protohemin IX. HM-RA HM-H used for reconstitution apomyoglobin from horse heart, yielding the Mb-RA Mb-H derivatives, respectively, protein. spectral, binding catalytic properties are significantly different those Mb. As shown MM MD calculations, these differences determined some local...
The spectroscopic, conformational, and reactivity characteristics of the T67R variant sperm whale myoglobin have been studied to assess effects introducing an arginine residue into distal side this protein, as occurs in active site heme peroxidases. overall circular dichroism (CD) NMR spectroscopic properties various derivatives protein are little affected by mutation. mutant contains a high-spin ferric ion with water molecule sixth ligand, which exhibits slightly enhanced acidity...
The EF-2 coding genes of the Archaea Pyrococcus woesei and Desulfurococcus mobilis were cloned sequenced. Global phylogenies inferred by alternative tree-making methods from available EF-2(G) sequence data contrasted with constructed more conserved but shorter EF-1 alpha(Tu) sequences. Both monophyly (sensu Hennig) their subdivision into kingdoms Crenarchaeota Euryarchaeota are consistently analysis sequences, usually at a high bootstrap confidence level. In contrast, tend to be inconsistent...
Protein engineering and cofactor replacement have been employed as tools to introduce/modulate peroxidase activity in sperm whale Mb (myoglobin). Based on the rationale that haem active sites are characterized by specific charged residues, crevice has modified host a haem-distalpropionate Arg residue proximal Asp, yielding T67R/S92D mutant. To code extra conformational mobility around haem, increase catalytic efficiency, mutant subsequently reconstituted with protohaem-l-histidine methyl...
The modification of human myoglobin (HMb) by reaction with nitrite and hydrogen peroxide has been investigated. This is important because NO(2) (-) H(2)O(2) are formed in vivo under conditions oxidative nitrative stress, where protein derivatization often observed. abundance HMb tissues the heart makes it a potential source target reactive species generated body. oxidant nitrating produced HMb/H(2)O(2)/NO(2) nitrogen dioxide peroxynitrite, which can react exogenous substrates endogenous...