A5066028581- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Otolaryngology and Infectious Diseases
- Cervical Cancer and HPV Research
- Bacterial Infections and Vaccines
- Obsessive-Compulsive Spectrum Disorders
- Cancer Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Inflammasome and immune disorders
- Peripheral Neuropathies and Disorders
- Oral and Maxillofacial Pathology
- Neurological diseases and metabolism
- Maternal and Neonatal Healthcare
- Tumors and Oncological Cases
- Cardiac Valve Diseases and Treatments
- T-cell and Retrovirus Studies
- Immune Response and Inflammation
- Plant Pathogenic Bacteria Studies
- Genetic Neurodegenerative Diseases
- Botulinum Toxin and Related Neurological Disorders
- Autoimmune Neurological Disorders and Treatments
Istituto Superiore di Sanità
2012-2024
Centre Hospitalier Universitaire de Liège
2022
Policlinico di Modena
2016
Ministero della Salute
2016
Sapienza University of Rome
1998-2004
A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for presence virulence factor genes, such as those collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (asa1 asa373), a surface (esp) two novel putative antigens (EF0591 EF3314). Apart some genes that were present in all (ace, efaA EF3314), gelE gene most common factor, although its did not...
ABSTRACT Group B streptococci (GBS) comprising three different sets of isolates (31 invasive, 36 noninvasive, and 24 colonizing isolates) were collected in Italy during the years 2002 to 2005. Clonal groups established by pulsed-field gel electrophoresis (PFGE), selected studied multilocus sequence typing (MLST). GBS also characterized classical molecular techniques for serotyping protein gene antibiotic resistance profiling. Some serotypes significantly associated with a particular isolate...
ABSTRACT Streptococcus pyogenes infections often fail to respond antibiotic therapy, leading persistent throat carriage and recurrent infections. Such failures cannot always be explained by the occurrence of resistance determinants, it has been suggested that S. may enter epithelial cells escape treatment. We investigated 289 strains isolated from different clinical sources evaluate their ability form biofilm as an alternative method treatment host defenses. Up 90% isolates, both invasive...
To investigate the epidemiology and characteristics of invasive group A streptococcal (GAS) disease over 11 years in Italy, this study compared emm types superantigen toxin genes speA speC as well erythromycin, clindamycin, tetracycline susceptibilities 207 GAS strains collected during two national enhanced surveillance periods (1994 to 1996 2003 2005) time between each set periods. The present demonstrated that emm1 were consistently responsible for about 20% infections, while variations...
The characteristics of group B streptococcus (GBS) neonatal disease in a period 7 years are reported.The estimation the GBS risk and prevention strategies adopted at delivery absence national guidelines was evaluated by analysis 3501 questionnaires. Notification 194 infections recorded. In addition, 115 strains from early-onset (EOD) late-onset disease, respectively, plus 320 pregnant women were analyzed molecular typing methods for antibiotic resistance.Preterm deliveries, precipitous labor...
Human papillomavirus (HPV) is associated with benign cutaneous or mucosal lesions and malignant tumours, but none of the HPV types has so far been related to skin tags. Skin biopsy specimens from 49 Caucasian patients suffering presence multiple soft fibromas were analysed by means dot blot hybridization polymerase chain reaction assays aimed at detecting all known types. The results revealed DNA type 6/11 in 88% tags examined. This result supports hypothesis that plays a part progression...
The recently documented presence of almost identical, small, non-self-transmissible, erm(T)-carrying plasmids in clonally unrelated erythromycin-resistant isolates Streptococcus pyogenes and agalactiae suggests that these somehow circulate the streptococcal population. objective this study was to characterize genetic element a clinical isolate dysgalactiae subsp. equisimilis (Sde5580) provide possible explanation for spread streptococci. Sde5580 investigated by plasmid analysis, PCR...
Abstract Repeated exposure to Group-A β-Haemolytic Streptococcus (GAS) may constitute a vulnerability factor in the onset and course of pediatric motor disturbances. GAS infections/colonization can stimulate production antibodies, which cross blood brain barrier, target selected areas (e.g. basal ganglia) exacerbate alterations. Here, we exposed developing SJL male mice four injections with homogenate evaluated following domains: coordination; general locomotion; repetitive behaviors;...
A recent increase in virulence of pathogenic Streptococcus dysgalactiae subsp . equisimilis (SDSE) has been widely proposed. Such an may be partly explained by the acquisition new traits horizontal gene transfer from related streptococci such as pyogenes (GAS) and agalactiae (GBS). collection 54 SDSE strains isolated Italy years 2000–2010 different sources (paediatric throat carriage, invasive non-invasive diseases) was characterized emm typing pulsed-field gel electrophoresis (PFGE)...
Streptococcus pyogenes (group A streptococci; GAS) recovered from paediatric pharyngitis (101 isolates) and asymptomatic children (79 in the same geographical area period, as well isolates collected during an enhanced national surveillance programme for GAS invasive diseases isolates), were screened incidence of streptococcal pyrogenic exotoxin (spe) genes speA speC, macrolide-resistance erm(B), erm(A) subclass erm(TR) mef(A), typed by emm sequencing. The gene was detected with comparable...
ABSTRACT A total of 161 Streptococcus pyogenes isolates from patients with invasive infections or asymptomatic carriers were examined for genes ( prtF1 , prtF2 and fba ) coding fibronectin-binding proteins to evaluate their involvement in the pathogenesis different streptococcal manifestations. We found no significant differences presence these three between two groups. Overall, gene was present similar percentages among strains both sources (61% versus 63%). Strains carrying slightly more...
Invasive infections by group B streptococci (iGBS) are the leading cause of sepsis and meningitis in first three months life worldwide. The clinical microbiological characteristics neonatal infant iGBS Italy during years 2015-2019 were investigated. Voluntary-based surveillance reported 191 cases (67 early-onset (EOD) 124 late-onset disease (LOD)) 89 bacterial isolates received. main manifestations (59.2%) followed (21.5%), bacteremia (12.0%) septic shock (6.3%). Hospitalized preterm babies...
Streptococcus agalactiae (group B streptococci, GBS) is responsible for severe infections in both neonates and adults. Currently, empiric antimicrobial therapy sepsis meningitis the combined use of penicillin gentamicin due to enhanced bactericidal activity. However, high-level resistance (HLGR) abrogates synergism. The rate HLGR was investigated within a dataset 433 GBS strains collected from cases invasive disease adults as well pregnant carriers. isolates (n = 20, 4.6%) presented with...
The characteristics of Group B Streptococcal (GBS) early onset (EOD) and late (LOD) neonatal infections in Italy were analyzed. Two periods considered, a first 3-years period (2007-2010), when notification GBS was enforced under the auspices Italian Ministry Health, second 1 year (2012) reporting on disease continued voluntary basis.A standardized form used to collect data cases disease. They included both maternal data.The two surveys underlined that preterm deliveries, precipitous labor...
Human papillomaviruses (HPVs) have been proposed to be the most important etiological factors for cervical cancer although different agents may act in conjunction. Herpes simplex virus type 2 (HSV-2) infection is considered as a possible cofactor malignant transformation. To examine influence of HSV-2 on HPV genes expression, CaSki cells bearing 60 600 copies HPV-16 DNA per cell were used model system. Twenty hours post mRNA transcripts early (E1, E2 and E6) late (L1) analysed by RT-PCR...
Group B Streptococcus (GBS, agalactiae) is a pathogen of increasing importance in adults. Severe and invasive cases non-pregnant adults were collected during the period 2015–2019 by voluntary-based surveillance. In total, 108 GBS strains phenotypically genotypically characterized for serotype, antimicrobial resistance, pili, surface protein genes, hyper-virulent adhesin hvgA. Patients divided into two age groups: (18–64 years; n = 32) older (≥65 72). The average was 70.8 years, with...