A5081311905- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Antibiotic Use and Resistance
- Hepatitis Viruses Studies and Epidemiology
- Listeria monocytogenes in Food Safety
- Mycobacterium research and diagnosis
- Yersinia bacterium, plague, ectoparasites research
- Digestive system and related health
- Bacterial Identification and Susceptibility Testing
- Medical and Biological Sciences
- Healthcare Systems and Public Health
- Economic and Technological Systems Analysis
- Zoonotic diseases and public health
- Research in Social Sciences
- Anorectal Disease Treatments and Outcomes
- HIV, TB, and STIs Epidemiology
- Urinary Tract Infections Management
Saint Petersburg Pasteur Institute
2015-2024
North-Western State Medical University named after I.I. Mechnikov
2020-2024
Ministry of Health of the Russian Federation
2020
Irkutsk Scientific Center of Surgery and Traumatology
2014
Introduction. Pathogenic strains of Escherichia coli are an important object surveillance within the One Health concept in wild, agriculture and human society. Migratory bird colonies high latitude avian may be points active intraspecies interspecies contact between different animal species, accompanied by spread pathogens. At same time, phylogeography E. relation to presence natural foci colibacillosis polar regions remains virtually unstudied. The aim this study was assess pathogenic...
Extended-spectrum beta-lactamases (ESBL) and AmpC producing-Escherichia coli have spread worldwide, but data about ESBL-producing-E. in the Northern Eastern regions of Europe is scant. The aim this study has been to describe phenotypical molecular epidemiology different ESBL/AmpC/Carbapenemases genes E. strains isolated from Baltic States (Estonia, Latvia, Lithuania), Norway St. Petersburg (Russia), determine predominant multilocus sequence type single nucleotide polymorphisms diversity...
Russia's national projects are an important tool for the socio-economic development of state, which includes all sectors country's economy. The purpose projects’ implementation is to improve economic performance, as well well-being citizens. Over past years, have shown their effectiveness. Hospitals, schools, kindergartens been built; a wide segment population has opportunity purchase housing on mortgage. agriculture equally important; roads built and repaired, thereby raising standard...
Introduction. Uropathogenic Escherichia coli (UPEC) are characterized by the ability to survive and reproduce in urinary tract due presence of specific virulence factors. In routine laboratory practice, detection diagnostically significant bacteriuria does not provide an idea level infection system (renal parenchyma, bladder), pathogenic potential strain progression chronicity infectious process, occurrence life-threatening conditions (urosepsis, meningitis). Objective. To characterize...
Introduction. Inflammatory bowel disease (IBD) is a common pathology that cannot be completely cured and requires lifelong therapy. One of the promising sparing therapeutic strategies use probiotics. Unlike Lactobacillus Bifidobacterium, which represent basis most classical probiotics, Bacillus spp. are spore-forming bacteria contributes to their resistance acidic environment stomach. It has been shown an effective drug for relief intestinal diseases with signs inflammation bacterial origin....
The aim of this study was to compare the prevalence different virulence factor (VF) genes in extended-spectrum beta-lactamase (ESBL) producing Escherichia coli strains isolated from Baltic Sea region. A total 432 phenotypically ESBL positive E. were collected 20 institutions located Estonia, Latvia, Lithuania, and region St. Petersburg Russia January May 2012 analyzed for phylogenetic group 23 VF genes. clinical material (urine, blood, wound, respiratory tract). Bacterial isolates compared...
Shiga toxin-producing Escherichia coli (STEC) food-borne infections are reported worldwide and represent a serious problem for public healthcare. In the Russian Federation there is little information on epidemiology etiology of STEC-infections as well molecular-genetic peculiarities STEC pathogens.Our aim was to describe outbreak hemorrhagic colitis (HC) along with hemolytic uremic syndrome (HUS), enterocolitis, acute gastroenteritis in children St. Petersburg 2013.Epidemiological,...
Acute intestinal infections caused by Escherichia coli affect the gastrointestinal tract, leading to development of diarrheal syndrome, intoxication, and, in some cases, generalization pathological process. Diarrheagenic E. (DEC) strains differ from non-pathogenic (commensal) presence specific virulence genes, pathogenesis characteristics, clinical and epidemiological manifestations diseases they cause. Based on determinants, 6 pathogenic DEC groups are distinguished: enteropathogenic,...
Abstract. We studied the prevalence of K. pneumoniae and E. coli resistance to extended spectrum cephalosporins carbapenems, isolated from patients eight hospitals in St-Petersburg January May, 2012. Prevalence cephalosporin resistant isolates varied different hospitals: – 7,8 50%, 25,4 88,4%. Isolates produced beta-lactamases СТХ-М, mainly СТХ-М-1, also СТХ-М-2 СТХ-М-9. Twenty two carbapenem-resistant strains (also other antimicrobials) were three hospitals. MALDI-TOF MS showed that...
The aim of the study is estimate opportunities local multi-prime PCR reagents kits in children enteric infections etiological diagnostics amongst patients with diarrhoea vs traditional bacteriological methods. We used 4 that provide multiple pathogens simultaneous indication one sample: 1) Rotavirus, Norovirus, Astrovirus; 2) Shigella spp./EIEC, Salmonella spp., Campylobacter spp.; 3) Yersinia enterocolitica and pseudotuberculosis; 4) E. coli: EIEC (enteroinvasive), EPEC (enteropathogenic),...
The gut microbiota of healthy people, living in different regions, may vary considerably. article presents the results comparative qualitative and quantitative study intestine aerobic anaerobic residents Republic Guinea (46) Russia (60). content microorganisms (Enterobacteriaceae, Enterococcus spp., Lactobacillus Bifidobacterium Staphylococcus Candida non-fermentative gram-negative bacteria others) 1.0 gram faeces was determined by bacteriological methods. Generic species identification...
Next-generation sequencing (NGS)-library preparation for whole-genome (WGS) starts with DNA fragmentation, and sonication is a physical approach used most often due to its simplicity reproducibility. However, the commercially available Covaris instrument has high price both device consumables. Here, we describe our in-house method of shearing by small (100-600 µm) glass beads an ultrasonic bath. The fragmentation conditions were optimized bacterial WGS ∼550-bp fragment size (the bath water...
The Сomamonas genus, described in 1985, included one species — terrigena. At present, the genus includes 21 species. ability of Comamonas to survive environmental objects (samples water, soil and plants), including hospital environment medical equipment, allows them be considered as opportunistic microorganisms. purpose research was study biological properties antimicrobial susceptibility kerstersii strains isolated from fecal samples healthy people living Saint Petersburg (Russian...
<title>Abstract</title> Paratyphoid B fever (PTB) is caused by an invasive lineage (phylogroup 1, PG1) of <italic>Salmonella enterica</italic> serotype Paratyphi (SPB). Here, we provide a genomic overview the population structure, geographic distribution, and evolution SPB PG1 analysing genomes from 568 historical contemporary isolates, obtained globally, between 1898 2021. We show that this pathogen existed in 13th century, subsequently diversifying into 11 lineages 38 genotypes with strong...
Introduction. Campylobacteriosis is among the leading causes of acute gastrointestinal infections. The severity campylobacteriosis and development long-term complications may be influenced by genotype pathogen, whose biological properties can affect immune response parameters. aim study was to identify common genotypes epidemic clones Campylobacter pathogens investigate characteristics disease. Materials methods. included 203 patients aged from 1 month 17 years with who underwent treatment...
Background.The issue of diarrheal diseases remains relevant for modern health care in all countries. Campylobacteriosis is the most common infectious disease with foodborne transmission and poultry meat a factor.Materials methods.724 items faeces sampled from patients syndrome 283 samples chickens raised on private farms five province were studied. For bacteriological method used selective media. Traditional routine tests (cell morphology, cytochrome oxidase, catalase, hydrolysis sodium...
Introduction. Diarrheal syndrome is the most characteristic symptom of HIV infection, which occurs in 70% patients and often fatal. The severity diarrheal syndrome, irrespective immune status, usually determined by specific microorganisms colonising gastrointestinal tract.
 objective this study to assess prevalence classical pathogens acute intestinal infections HIV-infected residents Chechen Republic (Grozny).
 Materials methods. Stool samples (n = 191) with a history were studied...
Introduction. Real-time PCR may be used along with loop isothermal amplification method (LAMP) allowing to conduct the study in 30–40 minutes diagnose typhoid fever. Several LAMP assay variations for detecting Salmonella enterica serovar Typhi have been described. The studies report that relevant primers were tested on strains specific Malaysia and China. We attempted evaluate described above identifying S. Russian Federation compare their sensitivity specificity each other. Materials...
Abstract Paratyphoid B fever (PTB) is caused by an invasive lineage (phylogroup 1, PG1) of Salmonella enterica serotype Paratyphi (SPB). However, little was known about the global population structure, geographic distribution, and evolution this pathogen. Here, we report a whole-genome analysis 568 historical contemporary SPB PG1 isolates, obtained globally, between 1898 2021. We show that pathogen existed in 13th century, subsequently diversifying into 11 lineages 38 genotypes with strong...
The aim of the work is to assess biological properties enteroaggregativeEscherichia coli(EAgEC) strain isolated from colon mucosa biopsy material a patient with ulcerative colitis, and develop new method for PCR identification ofE. colistrains. Materials methods.The study involved 46 patients verified colitis. Isolation 87E. colistrains was carried out by using obtained during standard endoscopic procedure. description biochemical bacterial combined molecular genetic detection virulence...
Objective. To analyze the frequency of concomitant pathology in HIV-infected outpatients Chechen Republic. Materials and methods. The study was conducted according to data "Republican Clinical Center for Infectious Diseases", Prevention Control AIDS" Ministry Health inpatient records 378 (male – 64.5%, female 35.5%) Diseases" Grozny period from 2013 2020 were analyzed. Results. As a result retrospective analysis, it found that chronic viral hepatitis C leads among causes HIVinfected...