A5028034050- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Salmonella and Campylobacter epidemiology
- Milk Quality and Mastitis in Dairy Cows
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Yersinia bacterium, plague, ectoparasites research
- Rabies epidemiology and control
- Aquaculture disease management and microbiota
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- Microbial infections and disease research
- Polysaccharides and Plant Cell Walls
- Enterobacteriaceae and Cronobacter Research
- Antifungal resistance and susceptibility
- Escherichia coli research studies
- Amino Acid Enzymes and Metabolism
- Drug-Induced Adverse Reactions
- Bacterial biofilms and quorum sensing
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2015-2024
Polish Academy of Sciences
2015-2024
Institute of Immunology
2019
Wroclaw University of Environmental and Life Sciences
2019
Heidelberg Institute for Theoretical Studies
2011
Campylobacter spp. is a major cause of foodborne diseases in humans, particularly when transmitted by the handling or consumption undercooked poultry meat. Most infections are self-limiting, but antimicrobial treatment (e.g., fluoroquinolones and macrolides) necessary severe prolonged cases. The indiscriminate use these drugs, both clinical medicine animal production, has impact on public health. aim present study was to identify strains, isolated from turkey broilers, using PCR...
Staphylococcus is one of the most frequently isolated genera opportunistic bacteria in animals and human beings. Staphylococci mammals mostly inhabit skin mucous membranes. The objectives study were to investigate distribution staphylococcal species healthy sick cats order find diagnostic markers. risk factors associated with colonization also explored. Isolates from (n=520) (n=67) identified at level using matrix-assisted laser desorption ionization time flight mass spectrometry (MALDI-TOF...
Elizabethkingia miricola is a rare Gram-negative bacterium found in water and clinical specimens. Typical culturing methods often misidentify spp. as Flavobacterium or Chryseobacterium. Although diagnosis based on samples taken from sterile sites, such blood, proper identification of this requires an expertise that goes beyond the capabilities typical laboratory. A 35-year-old woman diagnosed with common variable immunodeficiency was admitted to our center. Previous treatment antibiotics...
Twelve Staphylococcus borealis strains, isolated in Canada and Poland from milk of cows with intramammary infections, were characterized phenotypically (biochemical reactions on ID 32 STAPH Biolog Phenotype MicroArrays™ PM1 PM2A, ability biofilm production) genotypically (random amplified polymorphic DNA). In addition, a genomic comparison was done S. strains human porcine origin using the multilocus sequence typing (MLST) technique. The bovine isolates showed high degree phenotypic...
Abstract Staphylococcus pseudintermedius is a well-known coagulase-positive staphylococcus that mainly associated with the asymptomatic colonization of skin pets and mucous membranes. Little still known about occurrence S. in cats. The current study aimed to characterize isolates from sick healthy This was achieved by examining their antibiotic resistance properties, biofilm formation, genotype differences. Six hundred seventy-six cats were swabbed (595 81 cats). Thirty-five distinct 27...
Abstract Oral health is a window to patient’s general well-being. Balance in oral microbiome functions crucial for maintenance. A state of dysbiosis may lead variety local and systemic pathological conditions. The presence dental plaque related the majority infections. Proper hygiene most economic practice contributing prophylaxis. Aside from prophylactic treatments provided by practitioners, mouth rinses, containing antimicrobial agents, are one possible tools used care. Our study was...
The characterization of staphylococcal species that colonize pets is important to maintain animal health and minimize the risk transmission owners. Here, prevalence Staphylococcus spp. methicillin resistance was investigated in canine feline isolates, factors colonization were determined. Pets examined separated into four groups: (1) healthy dogs, (2) cats, (3) dogs (4) cats with clinical signs bacterial infections skin, mucous membranes, or wounds. Specimens collected by a veterinary...
Yersinia enterocolitica is widespread within the humans, pigs and wild boars. The low isolation rate of Y. from food or environmental clinical samples may be caused by limited sensitivity culture methods. main goal present study was identification presumptive isolates using MALDI TOF MS. difficult due to variability bacterial strains in terms biochemical characteristics. This work emphasizes necessity use multiple methods for zoonotic identification. Identification based on MS, verified...
The genus Lactobacillus includes, among others, casei, paracasei and rhamnosus, species that are collectively referred to as the casei group. Many studies have shown strains belonging this group may decrease lactose intolerance, effects of inflammatory bowel disease, diarrhea, constipation, food allergies even colon cancer. Moreover, evidences exists positive these bacteria on mucosal immunity blood cholesterol level. Because their beneficial influence human health, many them used additives...
(1) Background: Despite being considered a non-pathogenic yeast, recently, growing occurrence of Saccharomyces cerevisiae infections has been noted. There is little knowledge about the drug susceptibility this species. Therefore, objective research was to expand it and determine profile local collection clinical isolates (2) Methods: This study contained 55 identified as using MALDI-TOF method. The tested 10 antifungals (amphotericin B, flucytosine, fluconazole, voriconazole, posaconazole,...
Abstract Escherichia coli bacteria are an essential indicator in evaluations of environmental pollution, which is why they must be correctly identified. This study aimed to determine the applicability various methods for identifying E. strains samples. Bacterial preliminary selected on mFc and Chromocult media as were identified using MALDI Biotyper techniques, based presence genes characteristic ( uid A, usp yai O), well by 16S r RNA gene sequencing. The virulence antibiotic resistance...
The study was carried out in Polish goat population to estimate the prevalence of nasal cavity infection with various staphylococcal species including methicillin-resistant Staphylococcus aureus (MRSA), investigate potential permissive role small ruminant lentivirus (SRLV) and determine level clonality S. isolates.Nasal swabs blood samples were collected from 1300 clinically healthy adult goats 21 herds.Blood serologically screened for SRLV.Staphylococci isolated identified using classical...
Citrobacter strains are opportunistic pathogens often responsible for serious enteric as well extra-intestinal diseases, and therefore the O-antigenic scheme, still in use diagnostic identification, should be set proper serotyping. The structures of more than 30 different O-antigens (O-polysaccharide chains lipopolysaccharides) 43 O-serogroups have been elucidated so far. However, relationships between several heterogeneous serogroups need to clarified by immunochemical studies. These...
We compared the effectiveness of various methods for identification Staphylococcus spp. other than S. aureus isolated from intramammary infections cows on 3 dairy farms in Lower Silesia, Poland. A total 131 isolates belonging to 18 species were identified by sequence analysis 16S rRNA and dnaJ genes, as well using a commercial system (ID 32 STAPH; bioMérieux) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics). Sequencing gene was...
Lipopolysaccharide was extracted from cells of Salmonella enterica serovar Toucra O48 and, after mild acid hydrolysis (1% AcOH, 1 h, 100 °C or 0.1 m NaOH‐AcOH, pH 4.5, 5 °C), the O‐specific polysaccharide isolated and characterized. The core an oligosaccharide containing a fragment repeating unit linked to region were also obtained, depending on conditions. On basis sugar methylation analyses NMR spectroscopy products, biological shown be following trisaccharide: →4)‐α‐Neu p 5Ac(2→3)‐ l...
Using proteomics-based identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), we conducted the first analysis of composition endophytic bacteria isolated from different parts selected Epipactis species, i.e. buds, inflorescences and central part shoots, as well rhizomes. We identified aerobic anaerobic bacteria, including such taxa Bacillus spp., Clostridium Pseudomonas spp. Stenotrophomonas which may be considered promoting plant...
Salmonella is generally considered as a human pathogen causing typhoid fever and gastrointestinal infections called salmonellosis, with S. Enteritidis Typhimurium strains the main causative agents. enterica have wide host array including humans, birds, pigs, horses, dogs, cats, reptiles, amphibians insects. Up to 90% of reptiles are carriers one or more serovars Salmonella. Extraintestinal bacterial associated pose serious health threat humans. The import exotic species pet animals Europe...
Abstract Reptiles appear to be an important vector for Gram-negative pathogens, therefore, they are epidemiologically relevant. However, the composition of reptilian microbiota has been poorly recognized so far. The majority studies concern exotic reptiles as asymptomatic carriers Salmonella serovars. Studies other intestinal bacteria rare. Only recently, free-living European have investigated, however, on basis small samples, mainly in protected areas. Here, we aim investigate cloacal...