Abstract Introduction Staphylococci are still a challenge in veterinary medicine, as they one of the aetiological factors causing clinical and subclinical mastitis small ruminants. The aim study was to analyse occurrence staphylococci milk obtained from Świniarka (SW) Uhruska (UHR) sheep characterise their drug resistance virulence. Material Methods In total, 50 samples were collected ewes seven days after parturition. Drug susceptibility analysis performed based on Clinical Laboratory...

Summary Species differentiation within Trichophyton mentagrophytes complex group currently poses a major diagnostic challenge, with molecular methods increasingly supplementing classical identification based on the morphological and physiological properties of fungi. Diagnostic epidemiological research aimed at determining source means transmission dermatophytoses in both humans animals requires not only species isolates but also species. The study was conducted 24 originating various animal...

The objective of the study was to examine a population free-living carnivorous mammals most commonly found in Poland (red fox, beech marten, and raccoon) for occurrence bacteria that are potentially pathogenic humans other animal species determine their virulence potential (the presence selected genes). From total pool isolates obtained (n = 328), we 90 belonging pose greatest threat human health: Salmonella spp. 19; 4.51%), Yersinia enterocolitica 10; 2.37%), Listeria monocytogenes L....

Recent molecular methods for diagnosis of superficial mycoses have determined the need a rapid and easy method extracting DNA. The aim study was to determine growth conditions techniques DNA extraction Microsporum canis, Trichophyton mentagrophytes T. verrucosum. Samples were prepared each (phenol–chloroform, CTAB four different kits) all incubation periods (4, 7 10 days) cultures on solid in liquid medium. highest concentrations obtained using phenol–chloroform method. concentration...

Abstract Trichophyton verrucosum is a zoophilic fungus that the most frequent aetiological agent of dermatophytosis in cattle. During last few years, number cases T. from humans has been increasing constantly, which correlated with presence cattle‐rearing farms. We identified and analysed strains isolated Identification was carried out traditionally by correlating both clinical manifestations micro‐ macroscopic examination. To confirm species affiliation fully, molecular differentiation...

Abstract During the last few years, number of cases Trichophyton verrucosum isolation from humans suffering mycoses has been constantly increasing, which is correlated with presence an increasing outdoor breeding farms. Farmers and their families as well veterinarians technicians involved in handling animals are at a higher risk infection. One most important aims mycological diagnostics epidemiological analysis. Typically, history disease not sufficient to indicate reliably eliminate...

The aim of this study was to determine the antimicrobial resistance E. faecalis and faecium strains isolated from poultry carry out genotypic characterization thereof with ADSRRS-fingerprinting method (amplification DNA fragments surrounding rare restriction sites) analysis genetic relatedness between isolates different virulence determinants. Samples were collected 70 4-week-old chickens tested for Enterococcus. Minimum inhibitory concentrations 11 antimicrobials determined using broth...

Wild animals can serve as hosts, amplifiers or reservoirs for various zoonotic diseases. Most species of deer in highly fragmented agricultural landscapes, search out maximum cover from intrusive human activity. Hence, the likelihood zoonosis transmission is likely to increase more humans and wildlife interact. In our study, we conducted a comparative analysis bacteria isolated faeces red (Cervus elaphus) living their natural environment south-western Poland brought Hungary Slovakia under...

Dermatophyte infections are extremely frequent worldwide, and their epidemiological features distribution make them one of the most all over world. We identified analysed multiform T. mentagrophytes strains isolated from a silver fox (Vulpes vulpes) kept on breeding farm. Identification dermatophyte was carried out traditionally by correlating both clinical manifestations infection with micro- macroscopic examination. To confirm species affiliation fully, molecular differentiation methods...

Abstract Introduction The aim of the study was evaluation concentrations interleukin (IL)-1β, IL-8, IL-12β and tumour necrosis factor alpha (TNF-α) in serum milk cows with mastitis caused by Streptococcus agalactiae . Material Methods A total 60 samples from diseased 30 healthy were included study. Blood taken Holstein-Friesian three herds (two tie-stall one a free-stall housing system) Lublin Province Poland. cytokines blood quarter determined ELISA. Results levels IL-1β, TNF-α...

The species Lactococcus lactis is the bacterium extensively used in dairy industry. This generally recognized as safe which was added to European Food Safety Authority Qualified Presumption of list. major functions this fermentation are production lactic acid from lactose, citric and hydrolysis casein. But representatives producing bacteriocin substances can exert an inhibitory effect against spoilage bacteria. aim study test three lactococcal strains isolated raw goat milk for their...

The aim of this study was to characterize multidrug resistant E. faecalis strains from pigs local origin and analyse the relationship between resistance genotypic proteomic profiles by amplification DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI -TOF MS). From total pool Enterococcus spp. isolated 90 pigs, we selected 36 strains, which represented three different phenotypic profiles....

Fast and reliable identification of bacteria to at least the species level is currently basis for correct diagnosis appropriate treatment infections. This particularly important in case genus Enterococcus, whose resistance profile often correlated with their (e.g. vancomycin). In this study, we evaluated restriction endonuclease analysis 16S-23S rRNA gene intergenic transcribed spacer (ITS) region Enterococcus. The utility method was compared that phenotypic methods [biochemical evaluation...

The species

Ruminants are a group of animals that process and assimilate their food in unique manner. The functioning the digestive tract these is closely related to abundance composition microbes forestomach, which complex ecosystem bacteria, protozoa fungi. Microorganisms present rumen, particular effect on physiological processes body, influence animal’s physical condition state health. Microbiological examination rumen microbiota ecology hindered by lack selective growth media, as well difficulties...

In this study, we analysed phenotypic resistance profiles and their reflection in the genomic of Enterococcus spp. strains isolated from pigs raised on different farms.Samples were collected five pig farms (n=90 animals) tested for Enterococcus. MICs 12 antimicrobials determined using broth microdilution method, epidemiological molecular analysis belonging to selected species (faecalis, faecium hirae) was performed ADSRRS-fingerprinting (amplification DNA fragments surrounding rare...

In recent decades, the number of studies on occurrence resistant strains in wildlife animals has increased significantly, but data are still fragmentary. The aim this study was to evaluate drug resistance Escherichia coli isolated from wild carnivorous mammals, common Poland. Selective media with antimicrobials (tetracycline, kanamycin, chloramphenicol, and cefotaxime) were used for isolation. Of 53 isolates shown be distinct by amplification DNA fragments surrounding rare restriction...

We assessed the genomic diversity and relationship of 28 Astragalus glycyphyllos symbionts by three methodologies based on PCR reaction, i.e., RAPD, ERIC-PCR, AFLP. The AFLP method with one PstI restriction enzyme selective PstI-GC primer pair had a comparable discriminatory power as ERIC-PCR oneand these fingerprinting techniques distinguished among studied A. 18 17 genomotypes, respectively. RAPD was less in genomotyping rhizobia analyzed it efficiently resolved nine genomotypes. cluster...

Introduction. The possible transfer of antimicrobial resistance genes between Enterococcus faecium isolates from humans and different animal species, including those not covered by monitoring programs (e.g. pet wildlife), poses a serious threat to public health. Hypothesis/Gap Statement. Little is known about occurrence mechanisms phenomenon multidrug E. isolated various host species in Poland. Aim. aim the study was characterize multidrug-resistant animals (livestock, pets wildlife) terms...