A5058682142- Mass Spectrometry Techniques and Applications
- Crystallization and Solubility Studies
- Analytical Chemistry and Chromatography
- Insect and Arachnid Ecology and Behavior
- X-ray Diffraction in Crystallography
- Insect and Pesticide Research
- Plant and animal studies
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Natural product bioactivities and synthesis
- Neurobiology and Insect Physiology Research
- Synthetic Organic Chemistry Methods
- Fluorine in Organic Chemistry
- Phytochemistry and Biological Activities
- Plant biochemistry and biosynthesis
- Sesquiterpenes and Asteraceae Studies
- Glycosylation and Glycoproteins Research
- DNA and Nucleic Acid Chemistry
- Bioactive natural compounds
- Chemical Synthesis and Analysis
- Inorganic Fluorides and Related Compounds
- Plant Surface Properties and Treatments
- Carbohydrate Chemistry and Synthesis
- Biochemical Analysis and Sensing Techniques
- Synthesis and Properties of Aromatic Compounds
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2016-2025
Charles University
2003-2024
Czech Academy of Sciences
2010-2022
Czech University of Life Sciences Prague
2008-2022
Bridge University
2022
Okinawa Institute of Science and Technology Graduate University
2022
University of Kansas
2022
Ricardo Prague (Czechia)
2009
German Cancer Research Center
2009
Heidelberg University
2009
FAT/CD36 is a multifunctional glycoprotein that facilitates long-chain fatty acid (FA) uptake by cardiomyocytes and adipocytes of oxidized low density lipoproteins (oxLDL) macrophages. CD36 also mediates FA-induced signaling to increase intracellular calcium in various cell types. The membrane-impermeable sulfo-N-hydroxysuccinimidyl (NHS) ester oleate (SSO) irreversibly binds has been widely used inhibit CD36-dependent FA calcium. inhibition mechanism whether SSO modification involves the...
Abstract Fatty acid isomers are responsible for an under-reported lipidome diversity across all kingdoms of life. Isomers unsaturated fatty acids often masked in contemporary analysis by incomplete separation and the absence sufficiently diagnostic methods structure elucidation. Here, we introduce a comprehensive workflow, to discover through coupling liquid chromatography mass spectrometry with gas-phase ozonolysis double bonds. The workflow encompasses semi-automated data enables de novo...
Along with the recent progress in development of advanced synthetic methods, chemical community has witnessed an increasing interest promising carbon-rich materials. Among them, helicenes are unique 3D aromatic systems that inherently chiral and attractive for asymmetric catalysis, recognition material science. However, there have been only limited attempts at synthesizing long helicenes, which represent challenging targets. Here, we report on organometallic approach to derivatives...
The spatial distribution of neutral lipids and hydrocarbons has been imaged using MALDI-TOF mass spectrometry on intact plant insect surfaces, namely wings legs the gray flesh fly (Neobellieria bullata), common fruit (Drosophila melanogaster), leaves thale cress (Arabidopsis thaliana), date palm tree (Phoenix sp.). wax esters (WEs) saturated unsaturated (HCs) was visualized. samples were attached a target multiply sprayed with lithium or sodium 2,5-dihydroxybenzoate. deposits homogenous,...
Chromatographic separation of root extracts Morus alba and M. nigra led to the identification 2-arylbenzofurans moracin C (1), mulberrofuran Y (2), H (3), prenylated flavonoids kuwanon E (4), (5), sanggenon (6), cudraflavone B (7), morusinol (8), Diels–Alder adducts soroceal (9), (10). The cytotoxicity their antiphlogistic activity, determined as attenuation secretion TNF-α IL-1β inhibition NF-κB nuclear translocation in LPS-stimulated macrophages, were evaluated for compounds 1–10.
Geranyl flavones have been studied as compounds that potentially can be developed anti-inflammatory agents. A series of natural geranylated flavanones was isolated from Paulownia tomentosa fruits, and these were for their activity possible mechanism action. Two new characterized [paulownione C (17) tomentodiplacone O (20)], all the derivatives assayed ability to inhibit cyclooxygenases (COX-1 COX-2) 5-lipoxygenase (5-LOX). The tested showed variable degrees activity, with several them...
Cuticular hydrocarbons (CHCs) are hydrophobic compounds deposited on the arthropod cuticle that of functional significance with respect to stress tolerance, social interactions and mating dynamics. We characterized CHC profiles in natural populations Drosophila melanogaster at five levels: across a latitudinal transect eastern United States, as function developmental temperature during culture, seasonal time replicate years, rapid evolution experimental mesocosms field. Furthermore, we also...
Octahedral molybdenum cluster complexes bearing mitochondria-targeting terminal functions are attractive candidates for photodynamic applications and luminescent probes.
The fabrication and characterization of boron-doped diamond microelectrodes for use in electrochemical detection coupled with capillary electrophoresis (CE−EC) is discussed. were prepared by coating thin films polycrystalline on electrochemically sharpened platinum wires (76-, 25-, 10-μm diameter), using microwave-assisted chemical vapor deposition (CVD). diamond-coated attached to copper (current collectors), several methods explored insulate the cylindrical portion electrode: nail polish,...
Three novel structurally related pentadecapeptides, named lasioglossins, were isolated from the venom of eusocial bee Lasioglossum laticeps. Their primary sequences established as H-Val-Asn-Trp-Lys-Lys-Val-Leu-Gly-Lys-Ile-Ile-Lys-Val-Ala-Lys-NH(2) (LL-I), H-Val-Asn-Trp-Lys-Lys-Ile-Leu-Gly-Lys-Ile-Ile-Lys-Val-Ala-Lys-NH(2) (LL-II) and H-Val-Asn-Trp-Lys-Lys-Ile-Leu-Gly-Lys-Ile-Ile-Lys-Val-Val-Lys-NH(2) (LL-III). These lasioglossins exhibited potent antimicrobial activity against both...
By nature, defensive behavior is risky. In social insects, such more likely to occur in individuals whose potential for other tasks diminished. We show that workers of the termite Neocapritermes taracua develop an exceptional two-component suicidal apparatus consisting copper-containing protein crystals, stored external pouches, and internal salivary glands. During aggressive encounters, their bodies rupture, crystals react with gland secretion produce a toxic droplet. Both amount substances...
It has been more than 50 years since the discovery of dinucleoside polyphosphates (NpnNs) and yet their roles mechanisms action remain unclear. Here, we show that both methylated non-methylated NpnNs serve as RNA caps in Escherichia coli. are excellent substrates for T7 E. coli polymerases (RNAPs) efficiently initiate transcription. We demonstrate, enzymes 5'-pyrophosphohydrolase (RppH) bis(5'-nucleosyl)-tetraphosphatase (ApaH) able to remove NpnN-caps from RNA. ApaH is cleave all NpnN-caps,...
Termites (Blattodea: Isoptera) have evolved specialized defensive strategies for colony protection. Alarm communication enables workers to escape threats while soldiers are recruited the source of disturbance. Here, we study vibroacoustic and chemical alarm in wood roach Cryptocercus 20 termite species including seven nine families, all life-types, feeding nesting habits. Our multidisciplinary approach shows that vibratory signals represent an ethological synapomorphy termites Cryptocercus....
Wade’s rules are a well‐established tool for the description of geometry inorganic clusters. Among others, they state that decrease or increase in charge is always accompanied by change number skeletal electron pairs (SEPs). This work reports synthesis first cationic chalcogenaboranes closo‐[12‐X‐2‐IPr‐1‐EB11H10]BF4 (X = H, I; E S, Se 3a/b, 4a/b) featuring same SEP count as their neutral precursors, EB11H11, but bearing positive charge. ionisation significantly enhances activity towards...
Wade’s rules are a well‐established tool for the description of geometry inorganic clusters. Among others, they state that decrease or increase in charge is always accompanied by change number skeletal electron pairs (SEPs). This work reports synthesis first cationic chalcogenaboranes closo‐[12‐X‐2‐IPr‐1‐EB11H10]BF4 (X = H, I; E S, Se 3a/b, 4a/b) featuring same SEP count as their neutral precursors, EB11H11, but bearing positive charge. ionisation significantly enhances activity towards...
Phthalocyanines and their building blocks, isoindoline-1,3-diimines (diiminoisoindoles, DIIs), represent a structurally diverse class of compounds with the ability to make metal complexes perform in various fields from medicine photovoltaics homogeneous catalysis. According present study, monosubstituted diiminoisoindoles, higher homologues, can be effectively prepared by addition silylated lithium amides 1,2-dicyanobenzene followed mild protonolysis or condensation. An DII carbodiimides...
[reaction: see text] A new approach to nonracemic [7]helicene-like molecules has been developed. Stereoselective Co(I)-mediated [2 + 2 2] cycloisomerization of aromatic triynes containing an asymmetric carbon atom produces scaffolds in diastereomeric ratios up 100:0. This central-to-helical chirality transfer can be controlled by the absolute configuration at center and presence substituents.
A matter of concentration: Cyclic pseudorotaxanes are formed by the addition copper(I) ions to a ligand consisting ring containing chelating unit and, appended ring, rigid fragment another unit. The tetramer is as major product in concentrated solution, and analogous trimer prevails more dilute solution; both species connected through concentration-dependent slow equilibrium. Supporting information for this article available on WWW under...
Eight C-6-geranylflavonoids (1−8) were isolated from an ethanol extract of Paulownia tomentosa fruits. Tomentodiplacone (1), 3′-O-methyl-5′-hydroxydiplacone (2), 3′-O-methyl-5′-O-methyldiplacone (3), and tomentodiplacone B (4) obtained as new compounds, 3′-O-methyldiplacol (5) was for the first time a natural source. The structures these compounds determined by using mass spectrometry (including HRMS) 1D 2D NMR spectroscopy, absolute configurations circular dichroism. antibacterial...