A5047656276- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Botulinum Toxin and Related Neurological Disorders
- Forensic Toxicology and Drug Analysis
- Bacterial Identification and Susceptibility Testing
- HIV, Drug Use, Sexual Risk
- Orthopedic Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Sepsis Diagnosis and Treatment
- Neonatal Health and Biochemistry
- Nosocomial Infections in ICU
- Primary Care and Health Outcomes
- Leprosy Research and Treatment
- Fungal Infections and Studies
- Opioid Use Disorder Treatment
- Cystic Fibrosis Research Advances
- Bacillus and Francisella bacterial research
- Infectious Diseases and Tuberculosis
- Healthcare cost, quality, practices
- Antibiotics Pharmacokinetics and Efficacy
NHS Lothian
2011-2023
Edinburgh Royal Infirmary
2011-2018
Monklands Hospital
2010-2017
University of Glasgow
2007-2013
NHS Lanarkshire
2011-2013
University of the Witwatersrand
2010
Glasgow Royal Infirmary
2005-2007
London School of Hygiene & Tropical Medicine
2002
University of London
2002
Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis complete reference genome 81 draft sequences from clonal complex 180, the predominant clone in much world, found most sampled isolates belonged clade affected by few diversifying recombinations. However, indicate significant genetic variation has accumulated over complex's entire history. Two closely related genomes, one...
We describe associations between death from invasive pneumococcal disease (IPD) and particular serogroups sequence types (STs) determined by multilocus typing (MLST) using data Scotland. All IPD episodes where blood or cerebrospinal fluid (CSF) culture isolates were referred to the Scottish Haemophilus, Legionella, Meningococcal Pneumococcal Reference Laboratory (SHLMPRL) January 1992 February 2007 matched certification records General Register Office for This represented 5959 patients. The...
Streptococcus pneumoniae diseases are a rare but increasingly recognized trigger of atypical haemolytic uraemic syndrome (HUS) in young children and associated with higher mortality rate than diarrhoea-associated HUS. This study aimed to determine the importance neuraminidase A (NanA) genomic diversity pathogenesis pneumococcal HUS (pHUS). We investigated nanA gene sequence, expression, activity comparative hybridization invasive disease (IPD) isolates from patients pHUS control strains...
Contaminated blood cultures (BC) generate avoidable costs and prolong hospital stays. To measure our hospital's performance against the recommended standard of <3% BC contamination, we performed a prospective study.We prospectively determined frequency contaminated genuinely positive hospital-wide over seven months.Overall, 73 1,829 reviewed were (4.0%). However, distribution contamination was not uniform. Finding consistently higher incidence (11.7%) in emergency department (ED) than...
The 7-valent pneumococcal conjugate vaccine (Prevenar®, Wyeth; PCV7) was introduced to the UK paediatric immunisation schedule in 2006. This study investigates trends serotypes and multi locus sequence types (STs) among cases of invasive disease (IPD) Scotland prior to, following, introduction PCV7. Scottish Invasive Pneumococcal Disease Enhanced Surveillance has records all IPD since 1999. Cases diagnosed from blood or cerebrospinal fluid isolates until 2010 were analysed. Logistic poisson...
Spontaneous bacterial peritonitis, a potentially fatal complication of cirrhotic ascites, is diagnosed when the polymorphonuclear leucocyte count in ascitic fluid is>250/mm. Manual laboratory counting leucocytes is, however, labour-intensive, costly, results diagnostic delay and it not available all hospitals as part 'out-of-hours' service. Thus, rapid screening test for spontaneous peritonitis would be beneficial this condition. An exciting new development diagnosis use bedside reagent...
Abstract Background and Aims In April 2015, the UK government enacted a temporary class drug order (TCDO) on ethylphenidate in response to reported harms associated with its use, particular an outbreak of infections among people who inject drugs (PWID) Lothian, Scotland. This study assesses effect that TCDO had reducing most common identified during outbreak: Streptococcus pyogenes Staphylococcus aureus . Design The was split into pre‐intervention period (35 weeks) post‐intervention (26...
Giardiasis, caused by the intestinal protozoan parasite Giardia intestinalis (synonyms: G. lamblia, duodenalis), is one of most frequent parasites to infect Scottish population. Transmission infective cysts in faecal matter commonly via food and/or water. subdivided into assemblages, where clinical and epidemiological differences have been described between assemblages A B. This snapshot descriptive study examines 30 positive cases which 72% (n = 21) were shown be assemblage A, 14% 4) B 10%...
During 2007, a study of pneumococcal carriage in children was performed two towns (Trinidad and Riberalta) the Beni region Bolivian Amazon basin. Little has previously been reported regarding epidemiology Bolivia, no multilocus sequence typing (MLST) isolates from this documented. A rate 34% identified. Of 53 Streptococcus pneumoniae that survived transportation for serotyping, antibiotic susceptibility testing MLST, commonest serotypes were 6A (9%), 34 (8%), 4 (6%), 9A 10A 19A 23F (6%) 38...
Data from 4727 invasive isolates of Streptococcus pneumoniae submitted to the Scottish Haemophilus, Legionella, Meningococcus and Pneumococcus Reference Laboratory between 1999 2007 were analysed establish susceptibility profiles penicillin, erythromycin cefotaxime. Pneumococcal resistance penicillin over study period remained low, with only 0.2 % (n=7/4727) falling into this category (MIC ≥2 mg l(-1)). These have been sporadic, mainly represented serogroup 14 (ST9) 9 (ST156). In comparison,...
A 78-year-old man presented to hospital with new onset confusion and fever. The working diagnosis was of delirium due an infection unknown source, empirical i.v. antibiotic treatment given. Two days later, he deteriorated developed clinical features in keeping a total anterior circulation stroke. Brain imaging unremarkable. Blood cultures grew organism subsequently identified as Facklamia languida. Following broad-spectrum antibiotics, his condition improved. F. languida septicaemia, leading...
Aim: To investigate the effects of exposure a macrolide-resistant [erm (B)-expressing] strain Streptococcus pneumoniae (strain 2507) to clarithromycin (0.5 and 5 mg/L) added at outset 6 hours after initiation culture on early gene expression, energy metabolism, growth. Methods: Bacterial growth was determined by turbidometric colony counting procedures, metabolism measurement ATP, while analysis expression performed using reverse transcription-PCR sequencing. Results: Addition...
Injecting drug use is often associated with deep-seated infection. In Lothian in Scotland there has been a recent increase the of injected new psychoactive substances (NPS). Patients who have NPS presented Staphylococcus aureus bacteraemia (SAB) life-threatening complications. We describe unique case-series 14 episodes SAB ten patients. Users had significantly higher incidence endocarditis and cavitating pulmonary lesions (P < 0·05) compared to those inject only opiates. Cases people...
SUMMARY This retrospective, descriptive case-series reviews the clinical presentations and significant laboratory findings of patients diagnosed with treated for injectional anthrax (IA) since December 2009 at Monklands Hospital in Central Scotland represents largest series IA cases to be described from a single location. Twenty-one who fulfilled National Anthrax Control Team standardized case definitions confirmed, probable or possible are reported. All survived none required limb...
Following a cluster of haematology patients with carbapenem-resistant Klebsiella pneumoniae (CRKP) septicaemia, we initiated screening for rectal carriage CRKP and multidrug-resistant K. (MDRKP) in this patient group. Haematology inpatients submit swab once weekly. When plated onto chromogenic Brilliance™ UTI Agar (Oxoid), incubated overnight 10 µg ertapenem disc is identified semi-automated antibiotic susceptibility testing performed using the Vitek 2 analyser (Biomerieux). no zone...
In 2010, during an outbreak of anthrax affecting people who inject drugs, a heroin user aged 37 years presented with soft tissue infection. He subsequently was found to have anthrax. We describe his management and the difficulty in distinguishing from non-anthrax lesions. His full recovery, despite overall mortality 30% for injectional anthrax, demonstrates that some heroin-related cases can be managed predominately oral antibiotics minimal surgical intervention.
A nonneutropenic patient with treated low-grade non-Hodgkin’s (Follicular) lymphoma and secondary hypogammaglobulinemia recovered from pneumococcal pneumonia septicemia (serotype 7F; ST191) subsequent to influenza H1N1 (2009). Both infections were potentially vaccine preventable. The then developed meningitis due a serotype 35F pneumococcus unique Multilocus Sequence Type (ST7004) which was not Patient management influenced by host predisposition infection, antibiotic intolerance, poor...
Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher we support invest in the microbiology community, to benefit of everyone. This supports our principal goal develop, expand strengthen networks available members so that they can generate new knowledge about microbes ensure it is shared with other communities.
Knowledge of the epidemiology pneumococcal disease in Bolivia is sparse, and Multilocus Sequence Typing (MLST) isolates has not been previously possible. Beni state until recently a geographically isolated region Bolivian Amazon basin significant poverty. During June July 2007, we performed carriage study recruiting over 600 schoolchildren two towns state. Here, describe unique identification simultaneous nasopharyngeal multilocus sequence types with serotype 3 phenotype within single subject.