A5067926211- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Escherichia coli research studies
- Evolution and Genetic Dynamics
- Probiotics and Fermented Foods
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Parasitic Infections and Diagnostics
- Antimicrobial agents and applications
- Virus-based gene therapy research
- Estrogen and related hormone effects
- Steroid Chemistry and Biochemistry
- Plant Pathogenic Bacteria Studies
- Milk Quality and Mastitis in Dairy Cows
- Amino Acid Enzymes and Metabolism
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Viral Infectious Diseases and Gene Expression in Insects
- Infective Endocarditis Diagnosis and Management
- Pharmacogenetics and Drug Metabolism
Amager Hospital
2021
Copenhagen University Hospital
2021
Hvidovre Hospital
2016-2019
University of Copenhagen
2017-2019
Technical University of Denmark
2014-2017
Center for Systems Biology
2014
SIB Swiss Institute of Bioinformatics
2012
ETH Zurich
2012
Universitäts Frauenklinik
1970
Resistance arises quickly during chemotherapeutic selection and is particularly problematic long-term treatment regimens such as those for tuberculosis, HIV infections, or cancer. Although drug combination therapy reduces the evolution of resistance, pairs vary in their ability to do so. Thus, predictive models are needed rationally design resistance-limiting therapeutic regimens. Using adaptive evolution, we studied resistance response common pathogen Escherichia coli 5 different single...
As drug-resistant pathogens continue to emerge, combination therapy will increasingly be relied upon treat infections and help combat further development of multidrug resistance. At present a dichotomy exists between clinical practice, which favors therapeutically synergistic combinations, the scientific model emerging from in vitro experimental work, maintains that this interaction provides greater selective pressure toward resistance than other types. We sought extend current paradigm,...
The microbial ecosystem residing in the human gut is believed to play an important role horizontal exchange of virulence and antibiotic resistance genes that threatens health. While diversity gut-microorganisms their genetic content has been studied extensively, high-resolution insight into plasticity selective forces shaping individual genomes scarce. In a longitudinal study, we followed dynamics co-existing Escherichia coli lineages infant not receiving antibiotics. Using whole genome...
Elucidating the adaptive strategies and plasticity of bacterial genomes in situ is crucial for understanding epidemiology evolution pathogens threatening human health. While much known about Escherichia coli controlled laboratory environments, less effort has been made to elucidate genome dynamics E. its native settings. Here, we follow co-existing lineages infant gut during first year life. One lineage causes a urinary tract infection (UTI) experiences several alterations genomic content...
ABSTRACT Viruses spread between cells, tissues, and organisms by cell-free cell-cell transmissions. Both mechanisms enhance disease development, but it is difficult to distinguish them. Here, we analyzed the transmission mode of human adenovirus (HAdV) in monolayers epithelial cells wet laboratory experimentation a computer simulation. Using live-cell fluorescence microscopy replication-competent HAdV2 expressing green fluorescent protein, found that infection invariably occurred after cell...
To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and faecalis in clinical samples Denmark 2005-15 according to species van type, and, furthermore, investigate genetic relatedness E. isolates from 2015.During 2005-14, all VRE were tested for presence vanA/B/C genes by PCR. In 2015, whole-genome sequenced. From WGS data, MLST STs extracted silico . Core-genome (cgMLST) analysis was performed isolates.During 2005-15, 1043 vanA , 25 vanB 4 28 detected. The...
From 2012 to 2015, a sudden significant increase in vancomycin-resistant (vanA) Enterococcus faecium (VREfm) was observed the Capital Region of Denmark. Clonal relatedness VREfm and vancomycin-susceptible E. (VSEfm) investigated, transmission events between hospitals were identified pan-genome plasmids from largest clonal group characterized.WGS 1058 isolates carried out on Illumina platform perform SNP analysis identify pan-genome. One isolate also sequenced PacBio close genome....
In Danish hospitals, the number of infections caused by vancomycin-resistant Enterococcus faecium (VRE faecium) has dramatically increased in recent years. Hospital disinfectants are essential eliminating pathogenic microorganisms, and reduced susceptibility may contribute to hospital-associated infections. We have addressed whether clinical VRE display decreased biocide when compared vancomycin-sensitive (VSE isolates.In total 12 VSE 37 isolates obtained from hospitals over an extended time...
From 2012 to 2014, there has been a huge increase in vancomycin-resistant (vanA) Enterococcus faecium (VREfm) Copenhagen, Denmark, with 602 patients infected or colonized VREfm 2014 compared just 22 2012. The objective of this study was describe the genetic epidemiology assess contribution clonal spread and horizontal transfer vanA transposon (Tn1546) plasmid dissemination hospitals. from Denmark (2012–14) were whole-genome sequenced. structure determined Tn1546-like transposons...
Staphylococcus argenteus (S. argenteus) is a newly identified species that has been misidentified as aureus aureus) and clinically relevant. We 25 S. genomes in our collection of whole genome sequenced aureus. These were compared to publicly available phylogeny revealed seven clusters corresponding clonal complexes. The was found be different from the core analysis showed approximately 33% total gene pool shared between two species, at 90% homology level. An assessment mobile elements shows...
In a hospital-acquired infection with multidrug-resistant Elizabethkingia, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene analysis identified the pathogen as Elizabethkingia miricola. Whole-genome sequencing, genus-level core genome analysis, in silico DNA-DNA hybridization of 35 strains indicated that species taxonomy should be further explored.
Methicillin-resistant Staphylococcus aureus (MRSA) is the common name for a heterogeneous group of highly drug-resistant staphylococci. Two major MRSA classes are distinguished based on epidemiology, namely community-associated (CA) and hospital-associated (HA) MRSA. Notably, distinction CA- HA-MRSA molecular traits remains difficult due to high genomic plasticity S. aureus. Here we sought pinpoint global distinguishing features through comparative genome proteome analysis notorious lineage...
Denmark is a low prevalence country with regard to methicillin resistant Staphylococcus aureus (MRSA). In 2008 and 2014, two neonatal wards in the Copenhagen area experienced outbreaks typical community acquired MRSA belonging same spa type sequence (t015:ST45) both were PVL ACME negative. outbreak 1, isolates harbored SCCmec IVa 2 V. The clinical presentation differed between outbreaks, as none of five positive mothers 1 had mastitis vs. six (p < 0.02). To investigate if whole-genome...
We present the results of two European external quality assessments (EQAs) conducted in 2014 and 2016 under auspices Study Group on Staphylococci Staphylococcal Infections ESCMID. The objective was to assess performance participating centres characterizing Staphylococcus aureus using their standard in-house phenotypic genotypic protocols.A total 11 well-characterized blindly coded S. (n = 9), argenteus 1) capitis strains were distributed participants for analysis. Species identification, MIC...
ABSTRACT It is known that humans and pets living together can share the same Escherichia coli strain. In this study we assessed role played by household as reservoirs of E. strains causing urinary tract infection (UTI) in their owners. Fecal swabs from 15 dogs six cats with 19 patients community-acquired UTI were screened antimicrobial selective plating to detect displaying susceptibility profile UTI-causing Pet/patient pairs sharing indistinguishable pulsed-field gel electrophoresis (PFGE)...
Abstract Background Daptomycin is a cyclic lipopeptide used in the treatment of vancomycin-resistant Enterococcus faecium (VREfm). However, development daptomycin-resistant VREfm challenges nosocomial infections. Resistance mechanisms daptomycin are not fully understood. Here we analysed genomic changes leading to daptomycin-susceptible isolate becoming resistant after 40 days and linezolid combination therapy. Methods The two isogenic isolates (daptomycin-susceptible daptomycin-resistant)...