A5021510867- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Probiotics and Fermented Foods
- Gut microbiota and health
- Microbial Fuel Cells and Bioremediation
- Dermatology and Skin Diseases
- Soil Carbon and Nitrogen Dynamics
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Wastewater Treatment and Nitrogen Removal
- Plant Pathogenic Bacteria Studies
- Enzyme Production and Characterization
- Food Allergy and Anaphylaxis Research
- Methane Hydrates and Related Phenomena
- Acne and Rosacea Treatments and Effects
- Biocrusts and Microbial Ecology
- Metal Extraction and Bioleaching
- Plant Parasitism and Resistance
- Genetic and rare skin diseases.
- Radioactive element chemistry and processing
- Phytochemistry and biological activities of Ficus species
- Ecology and Vegetation Dynamics Studies
- Marine Bivalve and Aquaculture Studies
- Mine drainage and remediation techniques
Center for Environmental Health
2024
Helmholtz Zentrum München
2018-2024
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
2011-2018
Ludwig-Maximilians-Universität München
2012-2013
University of Bayreuth
2007-2011
Aarhus University
2007-2011
Abstract Intestinal bacteria influence mammalian physiology, but many types of are still uncharacterized. Moreover, reference strains mouse gut not easily available, although models extensively used in medical research. These major limitations for the investigation intestinal microbiomes and their interactions with diet host. It is thus important to study detail diversity functions microbiota members, including those colonizing intestine. To address these issues, we aimed at establishing...
ABSTRACT The distribution of membrane lipids 17 different strains representing 13 species subdivisions 1 and 3 the phylum Acidobacteria , a highly diverse Bacteria were examined by hydrolysis gas chromatography-mass spectrometry (MS) high-performance liquid chromatography-MS intact polar lipids. Upon both acid base hydrolyses total cell material, uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic ( iso -diabolic acid) was released in substantial amounts (22 to 43% fatty acids)...
ABSTRACT In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and physiologically active in situ . However, their individual functions interactions with higher taxa soil still unknown. Here, potential effects land use, properties, plant diversity, nanofauna acidobacterial community composition were studied by cultivation-independent methods grassland forest soils from three different regions Germany. The analysis 16S rRNA gene clone libraries representing...
13,16-Dimethyl octacosanedioic acid (iso-diabolic acid) is a major membrane-spanning lipid of subdivisions (SDs) 1, 3 and 4 the Acidobacteria, highly diverse phylum within Bacteria. It has been suggested that these lipids are potential building blocks for orphan bacterial glycerol dialkyl tetraethers (GDGT) occur widely in variety environmental settings. Here, we expand knowledge on occurrence iso-diabolic Acidobacteria by examining composition six strains belonging to SDs 6, 8, 10, 23 not...
Very few principles have been unraveled that explain the relationship between soil properties and biota across large spatial scales different land-use types. Here, we seek these general relationships using data from 52 differently managed grassland forest soils in three study regions spanning a latitudinal gradient Germany. We hypothesize that, after extraction of variation is explained by location type, still significant proportions abundance diversity biota. If predictors organisms were...
Zero-discharge marine aquaculture systems are an environmentally friendly alternative to conventional aquaculture. In these systems, water is purified and recycled via microbial biofilters. Here, quantitative data on nitrifier community structure of a trickling filter biofilm associated with recirculating system presented. Repeated rounds the full-cycle rRNA approach were necessary optimize DNA extraction probe set for FISH obtain reliable comprehensive picture ammonia-oxidizing community....
ABSTRACT Recently, iso -diabolic acid (13,16-dimethyl octacosanedioic acid) has been identified as a major membrane-spanning lipid of subdivisions 1 and 3 the Acidobacteria , highly diverse phylum within Bacteria . This finding pointed to potential source for bacterial glycerol dialkyl tetraethers that occur ubiquitously in peat, soil, lakes, hot springs. Here, we examined composition seven phylogenetically divergent strains subdivision 4 group is commonly encountered soil. Acid hydrolysis...
16S rRNA genes and transcripts of Acidobacteria were investigated in 57 grassland forest soils three different geographic regions. contributed 9-31% bacterial whereas the relative abundances respective 4-16%. The specific cellular content (determined as molar ratio : genes) ranged between 3 80, indicating a low situ growth rate. Correlations with flagellate numbers, vascular plant diversity soil respiration suggest that biotic interactions are important determinants transcript soils. While...
Acidobacteria constitute an abundant fraction of the soil microbial community and are currently divided into 26 subdivisions. Most cultivated members affiliated with subdivision 1, while only a few representatives subdivisions 3, 4, 8, 10 23 have been isolated described so far. Two novel isolates 4 were from subtropical savannah soils characterized in present work. Cells strains A22_HD_4H(T) Ac_23_E3(T) immotile rods that by binary fission. Colonies pink white, respectively. The aerobic...
A total of 17 Enterobacter-like isolates were obtained from blood during a septicaemia outbreak in neonatal unit, Tanzania, that could not be assigned based on phenotypic test to any existing Enterobacter species. Eight representative investigated detail. Fermentation characteristics, biochemical assays and fatty acid profiles for taxonomic analysis determined supplemented with information derived whole genome sequences. Phenotypic morphological tests revealed these Gram-stain-negative,...
Three novel strains of the phylum Acidobacteria (Ac_11_E3T, Ac_12_G8T and Ac_16_C4T) were isolated from Namibian semiarid savanna soils by a high-throughput cultivation approach using low-nutrient growth media. 16S rRNA gene sequence analysis placed all three in order Blastocatellales class Blastocatellia (Acidobacteria subdivision 4). However, similarities to their closest relative Pyrinomonas methylaliphatogenes K22T ≤90 %. Cells Ac_11_E3T, Ac_16_C4T Gram-staining-negative non-motile...
Abstract Background The identification of microbiota based on next-generation sequencing (NGS) extracted DNA has drastically improved our understanding the role microbial communities in health and disease. However, DNA-based microbiome analysis cannot per se differentiate between living dead microorganisms. In environments such as skin, host defense mechanisms including antimicrobial peptides low cutaneous pH result a high turnover, likely resulting numbers cells present releasing...
Three Gram-negative, non-spore-forming, encapsulated bacteria were isolated from a Namibian river-bank soil (strains 277T and 307) semiarid savannah (strain A2-1cT). 16S rRNA gene sequence analyses placed them within subdivision 1 of the Acidobacteria revealed 100 % similarity between strains 307 98.2 A2-1cT former two strains. The closest relatives with validly published names Telmatobacter bradus, Acidicapsa borealis ligni (94.7-95.9 to type strains). Cells all three rod-shaped motile...
Two Gram-type-positive, non-spore-forming bacteria, strains D16/0/H6T and A22/0/F9_1T, were isolated from Namibian semiarid savannah soils. 16S rRNA gene sequence analysis revealed 96.6 % identity between the two placed them within order Solirubrobacterales of class Thermoleophilia. The closest phylogenetic relatives with validly published names several genus Solirubrobacter species Conexibacter arvalis, pairwise similarities ≤ 94.0 %. Cells strain ovoid to rod-shaped, whereas A22/0/F9_1T...
A novel slow-growing bacterium, designated strain AW1220T, was isolated from agricultural floodplain soil sampled at Mashare (Kavango region, Namibia) by using a high-throughput cultivation approach. Strain AW1220T characterized as Gram-negative, non-motile, rod-shaped bacterium. Occasionally, some cells attained an unusual length of up to 35 µm. The showed positive responses for catalase and cytochrome-c oxidase divided binary fission and/or budding. had aerobic chemoorganoheterotrophic...
Abstract Background Darier’s disease (DD) is a genodermatosis caused by mutations of the ATP2A2 gene leading to disrupted keratinocyte adhesion. Recurrent episodes skin inflammation and infections with typical malodour in DD indicate role for microbial dysbiosis. Here, first time, we investigated microbiome using metabarcoding approach 115 swabs from 14 patients healthy volunteers. Furthermore, analyzed its changes context cutaneous transcriptome. Results We identified disease-specific loss...
Hopanoids are pentacyclic triterpenoid lipids synthesized by different bacterial groups. Methylated hopanoids were believed to be exclusively cyanobacteria and aerobic methanotrophs until the genes encoding for methylation at C-2 C-3 position (hpnP hpnR) found widespread in domain, invalidating their use as specific biomarkers. These have been detected genome of Acidobacterium 'Ca. Koribacter versatilis', but our knowledge synthesis presence biosynthetic pathway other member Acidobacteria is...
The mucosal immunity is crucial for restricting SARS-CoV-2 at its entry site. Intramuscularly applied vaccines against stimulate high levels of neutralizing Abs in serum, but the impact these intramuscular vaccinations on features less clear. Here, we analyzed kinetic and functional properties anti-SARS-CoV-2 saliva after vaccination with BNT162b2. We a total 24 healthy donors longitudinally up to 16 months. found that specific IgG appeared second vaccination, declined thereafter reappeared...
ABSTRACT Atopic dermatitis (AD) is the most common chronic inflammatory skin disease worldwide and characterized by a complex interplay with microbiota, Staphylococcus aureus often abnormally more abundant in AD patients than healthy individuals (HE). S. harbors diverse strains varied genetic compositions functionalities, which exhibit differential connections severity of AD. However, differences between HE remain unclear, variations seen at specific geographic level, implying spontaneous...
Both above- and below-ground parts of plants are constantly challenged with microbes interact closely them. Many plant-growth-promoting rhizobacteria, mostly interacting the plant's root system, enhance immunity in a process described as induced systemic resistance (ISR). Here, we characterized local (IR) triggered by model PGPR Pseudomonas simiae WCS417r (WCS417) Arabidopsis thaliana. Hydroponic application WCS417 to roots resulted propagation in/on leaves establishment IR. WCS417-triggered...