A5010690912- Prion Diseases and Protein Misfolding
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Immunotherapy and Immune Responses
- Veterinary medicine and infectious diseases
- Neurological diseases and metabolism
- Trace Elements in Health
- Animal Virus Infections Studies
- Multiple Myeloma Research and Treatments
- Antifungal resistance and susceptibility
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Virus-based gene therapy research
- Organ Transplantation Techniques and Outcomes
- Microbial infections and disease research
- Xenotransplantation and immune response
- Hepatitis Viruses Studies and Epidemiology
- Tuberculosis Research and Epidemiology
- Bone Metabolism and Diseases
- Retinoids in leukemia and cellular processes
- Animal Behavior and Welfare Studies
- Hematopoietic Stem Cell Transplantation
- Cytokine Signaling Pathways and Interactions
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2012-2023
Istituto Oncologico Veneto
2021
King's College London
2014
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2009
Italian Society of Physiotherapy
2008
Universidad Nacional de Córdoba
2008
Istituto Superiore di Sanità
2003-2005
Istituto Zooprofilattico Sperimentale delle Venezie
2002-2005
Centro Ricerche FIAT
2005
Fondazione Poliambulanza Istituto Ospedaliero
2005
Fetal membranes are tissues of particular interest for several reasons, including their role in preventing rejection the fetus and early embryologic origin. which may entail progenitor potential. The immunologic reactivity transplantation potential amnion chorion cells, however, remain to be elucidated.Amnion cells were isolated from human term placenta characterized by immunohistochemistry, flow cytometric analysis, expression profile relevant genes. immunomodulatory characteristics these...
Since summer 2010, numerous cases of Rabbit Haemorrhagic Disease (RHD) have been reported in north-western France both rabbitries, affecting RHD-vaccinated rabbits, and wild populations. We demonstrate that the aetiological agent was a lagovirus phylogenetically distinct from other lagoviruses which presents unique antigenic profile. Experimental results show disease differs RHD terms duration, mortality rates, higher occurrence subacute/chronic forms partial cross-protection occurs between...
Fetal membranes (amnion and chorion) have recently raised significant attention as potential sources of stem cells. We demonstrated that cells derived from human term placenta show cell phenotype, high plasticity, display low immunogenicity both in vitro vivo. Moreover, placenta-derived cells, after xenotransplantation, are able to engraft solid organs including the lung. On these bases, we studied effects fetal membrane-derived on a mouse model bleomycin-induced lung fibrosis. were infused...
Atypical neuropathological and molecular phenotypes of bovine spongiform encephalopathy (BSE) have recently been identified in different countries. One these phenotypes, named "amyloidotic" (BASE), differs from classical BSE for the occurrence a distinct type disease-associated prion protein (PrP), termed PrPSc, presence PrP amyloid plaques. Here, we show that agents responsible BASE possess biological properties upon transmission to transgenic mice expressing inbred lines nontransgenic...
Recently a bio-artificial liver (BAL) system has been developed at the Academic Medical Center (AMC) of Amsterdam to bridge patients with acute failure (ALF) orthotopic transplantation (OLT). After successful testing AMC-BAL in rodents and pigs ALF, phase I study ALF waiting for (OLT) was started Italy. We present safety outcome first 7 aged 21–56 years coma grade III or IV. The total treatment time ranged from 8 35 hours. Three received 2 treatments two different BAL's within three days....
The disease phenotype of bovine spongiform encephalopathy (BSE) and the molecular/ biological properties its prion strain, including host range characteristics BSE-related disorders, have been extensively studied since discovery in 1986. In recent years, systematic testing brains cattle coming to slaughter resulted identification at least two atypical forms BSE. These emerging disorders are characterized by novel conformers pathological protein (PrP(TSE)), named high-type (BSE-H) low-type...
RABBIT haemorrhagic disease (RHD) is an acute and lethal form of viral hepatitis in rabbits (Oryctolagus cuniculus) with a mortality rate adults ranging from 70 per cent to 100 cent. RHD was first reported China 1984, Europe 1986, where it caused severe losses rabbit, Australia 1996 (Abrantes others 2012). by the lagovirus virus (RHDV) family Caliciviridae. In course its evolution, RHDV split into six genotypes (Kerr 2009), all highly pathogenic virulent. Genotype 6 antigenic subtype (RHDVa)...
Susceptibility of sheep to scrapie, a transmissible spongiform encephalopathy small ruminants, is strongly influenced by polymorphisms the prion protein gene (PRNP). Breeding programs have been implemented increase scrapie resistance in populations; though desirable, similar approach has not yet applied goats. European studies now suggested that several can modulate susceptibility goats: particular, PRNP variant K222 associated with case-control Italy, France and Greece. In this study we...
Hepatitis E virus (HEV) is responsible for sporadic acute hepatitis in developed countries, where the infection acquired probably through ingestion of contaminated food, addition to travel-related cases. In this study, circulation HEV wild boar from nine Italian provinces was evaluated. An overall seroprevalence 10.2% found, although there were differences among provinces, while no samples positive RNA detection. This study indicates an active populations and suggests consider zoonotic risk...
Orthotopic liver transplantation (OLT) is the only effective therapeutic modality in severe acute hepatic failure (AHF). The scarcity of organs for leads to an urgent necessity temporary support treatments AHF patients. A hepatocyte-based bioartificial (BAL) under investigation with main purpose serve as bridging treatment until a becomes available OLT, or promote spontaneous regeneration. We developed novel radial-flow bioreactor (RFB) three-dimensional, high-density hepatocyte culture and...
SUMMARY Goodpasture's disease is a rare form of glomerulonephritis characterized by the production autoantibodies to glomerular basement membrane (GBM). In order understand development autoimmunity GBM, it important examine mechanisms underlying T cell responses autoantigen. A MoAb PI, with same specificity as patients’ autoantibodies, was used affinity-purify antigen from collagenase-digested human GBM. This material enriched in NCI domain α3 chain type IV collagen (α3(IV)NC1), known be...
MPPS stimulation of human lymphocytes is characterized by a 2-way cooperation phenomenon, i.e., T help B cells and cells. In this system suppressor are generated after 6 days culture. They were as Ia- Their activity affected both cell stimulation, it may involve 2 different mechanisms: direct suppression, interaction with soluble helper factors.
Purified human T and B lymphocytes were tested for their ability to show a mitogenic response purified polysaccharide (MPPS) extracted from C. albicans. It was found that neither nor cells alone could respond MPPS. When mixture of these two types tested, normal (comparable the one unfractionated peripheral blood lymphocytes) observed. This not affected by prior depletion macrophages. Results obtained with or mixed mitomycin C-treated indicated reciprocal helper effect exists between in be...
The prevalence of the hepatitis E virus (HEV) infection in pigs Northern Italy was serologically examined. survey carried out on 39 farms: 17 farrow-to-feeder, 10 farrow-to-finish, and 12 fattening enterprises. There were 1,422 sera that tested using commercial indirect ELISA. This method originally developed for testing human adapted analysis pig sera. All farms except one (97.43%) 714 samples (50.21%) resulted positive anti-HEV IgG antibodies. study confirms HEV is widespread might be...
Because of the frequent exposure cattle to mycobacteria avium/intracellulare group, an investigation was carried out into possible repercussions thereof on diagnosis bovine tuberculosis. Three calves from a tuberculosis‐free herd, scored avian reactors in γ‐interferon assay for tuberculosis, were sedated and inoculated endotracheally with virulent Mycobacterium bovis strain. Then, three other housed above donor calves. isolated nasal swabs infected, On these samples, TB complex‐specific...
The amyloidotic form of bovine spongiform encephalopathy (BSE) termed BASE is caused by a prion strain whose biological properties differ from those typical BSE, resulting in clinically and pathologically distinct phenotype. Whether peripheral tissues BASE-affected cattle contain infectivity unknown. This critical issue since the readily transmissible to variety hosts including primates, suggesting that humans may be susceptible. We carried out bioassays transgenic mice overexpressing PrP...