A5071891829- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Amyotrophic Lateral Sclerosis Research
- RNA regulation and disease
- Curcumin's Biomedical Applications
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- MicroRNA in disease regulation
- Neurogenetic and Muscular Disorders Research
- Parkinson's Disease Mechanisms and Treatments
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Nicotinic Acetylcholine Receptors Study
- Cancer, Lipids, and Metabolism
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Multiple Sclerosis Research Studies
- S100 Proteins and Annexins
- Neurological diseases and metabolism
- COVID-19 Clinical Research Studies
- Fibroblast Growth Factor Research
University of Milan
2022-2024
University of Pavia
2018-2022
Italian institute for Genomic Medicine
2020-2021
Center for Genomic Science
2018-2021
Alteration in RNA metabolism, concerning both coding and long non-coding RNAs (lncRNAs), may play an important role Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed a whole transcriptome RNA-seq analysis to investigate the regulation of Sporadic ALS patients (SALS), mutated (FUS, TARDBP SOD1) matched controls Peripheral Blood Mononuclear Cells (PBMC). Selected transcripts were validated spinal cord tissues. A total 293 differentially expressed (DE) lncRNAs was...
SARS-Cov-2 infection is frequently associated with Nervous System manifestations. However, it not clear how SARS-CoV-2 can cause neurological dysfunctions and which molecular processes are affected in the brain. In this work, we examined frontal cortex tissue of patients who died COVID-19 for presence SARS-CoV-2, comparing qRT-PCR ddPCR. We also investigated transcriptomic profile from matched controls by RNA-seq analysis to characterize transcriptional signature. Our data showed that could...
Antisense RNAs (asRNAs) represent an underappreciated yet crucial layer of gene expression regulation. Generally thought to modulate their sense genes in cis through sequence complementarity or act transcription, asRNAs can also regulate different molecular targets trans, the nucleus cytoplasm. Here, we performed in-depth characterization NFYC 1 (NFYC-AS1), asRNA transcribed head-to-head subunit proliferation-associated NF-Y transcription factor. Our results show that NFYC-AS1 is a...
Alpha-synuclein, encoded by the SNCA gene, is a pivotal protein implicated in pathogenesis of synucleinopathies, including Parkinson's disease. Current approaches for modulating alpha-synuclein levels involve antisense nucleotides, siRNAs, and small molecules targeting SNCA's 5′-UTR mRNA. Here, we propose groundbreaking strategy G-quadruplex structures to effectively modulate gene expression lowering amount. Novel sequences, identified on gene's transcription starting site mRNAs, were...
Alzheimer’s disease (AD), Parkinson’s (PD), and amyotrophic lateral sclerosis (ALS) are neurodegenerative disorders characterized by a progressive degeneration of the central or peripheral nervous systems. A role RNA metabolism has emerged in these diseases, concerning mRNAs processing non-coding RNAs biogenesis. We aimed to identify possible common grounds differences dysregulated pathways AD, PD, ALS. To do so, we performed RNA-seq analysis investigate deregulation both coding long...
Abstract Coding and long non-coding RNA (lncRNA) metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we present a dataset obtained via Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from sporadic mutated ALS patients (mutations FUS , TARDBP SOD1 VCP genes) healthy controls. This allows the whole-transcriptome characterization of PBMCs content, both terms coding RNAs, order to compare disease state controls,...
Abstract SNCA protein product, α‐synuclein, is widely renowned for its role in synaptogenesis and implication both aging Parkinson's disease (PD), but research efforts are still needed to elucidate physiological functions mechanisms of regulation. In this work, we aim characterize SNCA‐AS1, antisense transcript the gene, implications cellular processes. The overexpression SNCA‐AS1 upregulates α‐synuclein and, through RNA‐sequencing analysis, investigated transcriptomic changes which genes...
Deregulation of transcription in the pathogenesis sporadic Amyotrophic Lateral Sclerosis (sALS) is taking central stage with RNA-sequencing analyses from sALS patients tissues highlighting numerous deregulated long non-coding RNAs (lncRNAs). The oncogenic lncRNA ZEB1-AS1 strongly downregulated peripheral blood mononuclear cells patients. In addition, cancer-derived cell lines, belongs to a negative feedback loop regulation hsa-miR-200c, acting as molecular sponge for this miRNA. role has not...
Abstract The central nervous system (CNS) is surrounded by three membranes called meninges. Specialised fibroblasts, originating from the mesoderm and neural crest, primarily populate meninges serve as a binding agent. Our goal was to compare fibroblasts skin obtained same human‐aged donors, exploring their molecular cellular characteristics related CNS functions. We isolated meningeal (MFs) brain donors (SFs) subjects. A functional analysis performed measuring cell appearance, metabolic...
ABSTRACT Alpha-synuclein, encoded by the SNCA gene, is a pivotal protein implicated in pathogenesis of synucleinopathies, including Parkinson’s disease. Current approaches for modulating alpha-synuclein levels involve antisense nucleotides, siRNAs, and small molecules targeting SNCA’s 5’-UTR mRNA. Here, we propose groundbreaking strategy G-quadruplex structures to effectively modulate gene expression lowering amount. Novel sequences, identified on gene’s transcription starting site mRNAs,...
The multitasking nature of lncRNAs allows them to play a central role in both physiological and pathological conditions. Often the same lncRNA can participate different diseases. Specifically, MYC-induced Long non-Coding RNA MINCR is upregulated various cancer types, while downregulated Amyotrophic Lateral Sclerosis patients. Therefore, this work aims investigate potential mechanisms action its implications neurodegeneration relation expression levels SH-SY5Y cells through RNA-sequencing...
Bisdemethoxycurcumin (BDC) might be an inflammation inhibitor in Alzheimer's Disease (AD). However, BDC is almost insoluble water, poorly absorbed by the organism, and degrades rapidly. We thus developed a new nanoformulation of based on H-Ferritin nanocages (BDC-HFn).We tested BDC-HFn solubility, stability, ability to cross blood-brain barrier (BBB) model. effect AD control (CTR) PBMCs evaluate transcriptomic profile RNA-seq.We with diameter 12 nm improve solubility stability. The...
Alzheimer's disease (AD) is a chronic neurodegenerative disorder that associated with the most common type of dementia and characterized by presence deposits protein fragment beta-amyloid (Aβ) in brain. The natural product mixture curcuminoids improves certain defects innate immune cells AD patients may selectively enhance Aβ phagocytosis alteration gene transcription. In this work, we evaluated protective effects from investigating effect panel on NF-kB BACE1 signaling pathways expression...
Superoxide dismutase 1 (SOD1) is one of the causative genes associated with amyotrophic lateral sclerosis (ALS), a neurodegenerative disorder. SOD1 aggregation contributes to ALS pathogenesis. A fraction protein localized in nucleus (nSOD1), where it seems be involved regulation participating oxidative stress response and DNA repair. Peripheral blood mononuclear cells (PBMCs) were collected from sporadic (sALS) patients (n = 18) healthy controls 12) perform RNA-sequencing experiments...
Obesity is a multifactorial disease presenting sex-related differences including adipocyte functions, sex hormone effects, genetics, and metabolic inflammation. These can influence individuals' risk for dysfunctions, with an urgent need to perform sex-based analysis improve prevention, treatment, rehabilitation programs. This research work aimed at characterizing the transcriptional present in subcutaneous adipose tissue (SAT) of five obesity affected men versus women, additional focus on...
Obesity is a major risk factor for large number of secondary diseases, including cancer. Specific insights into the role gender differences and comorbidities, such as type 2 diabetes (T2D) cancer risk, are yet to be fully identified. The aim this study thus find correlation between transcriptional deregulation present in subcutaneous adipose tissue obese patients oncogenic signature multiple cancers, presence T2D, considering differences. (SAT) five healthy, normal-weight women, women with...
3D cell cultures are becoming more and important in the field of regenerative medicine due to their ability mimic cellular physiological microenvironment. Among different types scaffolds, we focus on Nichoid, a miniaturized scaffold with structure inspired by natural staminal niche. The Nichoid can activate responses simply subjecting cells mechanical stimuli. This kind influence results morphology organization, but molecular bases these changes remain largely unknown. Through RNA-Seq...
Non-coding RNAs show relevant implications in various biological and pathological processes. Thus, understanding the of these molecules stem cell biology still represents a major challenge. The aim this work is to study transcriptional dysregulation 357 non-coding genes, found through RNA-Seq approach, murine neural precursor cells expanded inside 3D micro-scaffold Nichoid versus standard culture conditions. Through weighted co-expression network analysis functional enrichment, we highlight...
Obesity is a complex disease with multifactorial causes, and its prevalence becoming serious health crisis. For this reason, there crucial need to identify novel targets players. With aim in mind, we analyzed via RNA-sequencing the subcutaneous adipose tissue of normal weight obesity-affected women, highlighting differential expression two tissues. We specifically focused on long non-coding RNAs, as 6 these emerged dysregulated diseased-tissue (COL4A2-AS2, RPS21-AS, PELATON, ITGB2-AS1,...
In this data article, we present the dataset from RNA-Seq analysis of subcutaneous adipose tissue collected 5 healthy normal weight women (NW, age 37 ± 6.7 years, BMI 24.3 0.9 kg/m2) and obese (OBF, 41 12.5 38.2 4.6 kg/m2). Raw obtained Illumina NextSeq 500 sequencer were processed through BlueBee® Genomics Platform while differential expression was performed with DESeq2 R package deposited in GEO public repository GSE166047 as accession number. Specifically, 20 samples divided between NW...