A5030733029- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Acute Lymphoblastic Leukemia research
- Immunodeficiency and Autoimmune Disorders
- Acute Myeloid Leukemia Research
- Renal Transplantation Outcomes and Treatments
- Mesenchymal stem cell research
- Cytomegalovirus and herpesvirus research
- Polyomavirus and related diseases
- Diabetes and associated disorders
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Chronic Myeloid Leukemia Treatments
- Transplantation: Methods and Outcomes
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Cell Adhesion Molecules Research
- Blood groups and transfusion
- Lymphoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
University of Washington
2012-2023
Fred Hutch Cancer Center
2014-2023
Seattle Cancer Care Alliance
2003-2019
Cancer Research Center
1989-2018
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2002-2018
Seattle University
1996-2018
United States Geological Survey
2006-2017
Roskilde University
2017
University of Minnesota
1967-2016
Indiana University – Purdue University Indianapolis
2016
We treated 93 patients who had acute non-lymphoblastic leukemia in the first remission or chronic myelocytic phase (median age, 30 years) with high-dose cyclophosphamide and fractionated total-body irradiation, followed by infusion of marrow from an HLA-identical sibling. To evaluate post-grafting prophylaxis for graft versus host disease, we studied these a sequential, prospective, randomized trial that compared effect combination methotrexate cyclosporine (n = 43) alone 50). All evidence...
Allogeneic bone marrow transplantation is curative in a substantial number of patients with hematologic cancers, marrow-failure disorders, immunodeficiency syndromes, and certain metabolic diseases. Unfortunately, only 25 to 30 percent potential recipients have HLA-identical siblings who can act as donors. In 1986 the National Marrow Donor Program was created United States facilitate finding procurement suitable from unrelated donors for lacking related donors.During first four years...
Marrow transplantation has generally been limited to patients with a sibling who is genotypically identical for HLA. In study of the acceptable limits HLA incompatibility, 105 consecutive hematologic cancers received marrow grafts from haploidentical donors (study group) were compared 728 similar concurrently receiving siblings (control group). The unshared haplotypes differed variably: 12 phenotypically but not HLA-A, HLA-B, and HLA-D; 63 at one locus (A, B, or D); 24 two loci; 6 three. A...
We report on the production of tumor necrosis factor (TNF)-alpha and TNF-beta by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- DQw1-positive donors frequently exhibit low TNF-alpha, whereas DR3- DR4-positive subjects show high levels TNF-alpha production. No correlation between HLA-A, -B, -C genotype was found. relevance this quantitative polymorphism to genetic...
Chronic myeloid leukemia can be cured by marrow transplantation from an HLA-identical sibling donor. The use of transplants unrelated donors is option for the 70 percent patients without sibling, but morbidity and mortality associated with such have been cause concern. We analyzed safety efficacy treatment chronic identified variables that predict a favorable outcome.
The period of neutropenia after autologous bone marrow transplantation results in substantial morbidity and mortality. previous phase I-II clinical trials suggest that recombinant human granulocytemacrophage colony-stimulating factor (rhGM-CSF) may accelerate neutrophil recovery thereby reduce complications patients transplantation.
The specificity of T lymphocyte activation is determined by engagement the cell receptor (TCR) peptide/major histocompatibility complexes expressed on antigen-presenting (APC). Lacking costimulation accessory molecules APC, proliferation does not occur and unresponsiveness to subsequent antigenic stimulus induced. B7/BB1 APCs binds CD28 CTLA-4 cells, provides a costimulus for proliferation. Here, we show that prolonged, specific hyporesponsiveness restimulation achieved blocking interaction...
Following the decision to hold their next full meeting after 14th International Histocompatibility Workshop in 2005, WHO Nomenclature Committee for Factors of HLA System has decided publish an interim report listing updated tables alleles including those assigned since publication last 2002 (1). The named during period follow principles established previous reports (1–17). As emphasized reports, there are required conditions acceptance new sequences official names. Where a sequence is...
Toll-like receptors (TLRs) are essential components of the immune response to fungal pathogens. We examined role TLR polymorphisms in conferring a risk invasive aspergillosis among recipients allogeneic hematopoietic-cell transplants.We analyzed 20 single-nucleotide (SNPs) toll-like receptor 2 gene (TLR2), 3 (TLR3), 4 (TLR4), and 9 (TLR9) cohort 336 transplants their unrelated donors. The was assessed with use multivariate Cox regression analysis. analysis replicated validation study...
One hundred thirty patients with severe aplastic anemia were conditioned cyclophosphamide for transplantation of marrow from HLA-identical siblings. The selected the present analysis according to criterion sustained engraftment. Of 130 patients, 97 are now alive between 1.4 and 11 years (median, 5) after transplantation. Twenty-nine thirty-three who died had either acute or chronic graft-versus-host disease (GVHD). Our was directed at identifying factors predicting GVHD survival in patients....
We describe a new monoclonal murine antibody that reacts with 50,000-mol wt polypeptide appears to be present on all E-rosetting cells. conclude this antigen is either identical or closely associated the E receptor because of (a) high degree concordance between E-rosette formation and 9.6 expression, (b) inhibition rosette by preincubation cells antibody, (c) observed failure lysostripped form E-rosettes. This last finding suggests cocapping receptor.
Graft-versus-host disease (GVHD) and infection are major complications of allogeneic bone marrow transplantation. Since intravenous immunoglobulin has shown benefit in several immunodeficiency autoimmune disorders, we studied its antimicrobial immunomodulatory role after
Polymorphisms in cytokine genes can influence immune responses, inflammation, and tissue injury may affect the outcome of hematopoietic stem-cell transplantation.
The occurrence of graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation for leukemia is thought to decrease the probability recurrence. To study this effect (called adoptive immunotherapy) we modified prophylaxis GVHD in patients with advanced hematologic neoplasms (mostly leukemia) who received transplants. Patients under 30 years age were randomly assigned one three regimens post-transplantation immunosuppression: Group I (n = 44) a standard course methotrexate 102...
PURPOSE Hematopoietic cell transplantation can cure hematologic malignancies and other diseases, but this treatment also cause late complications. Previous studies have evaluated the cumulative effects of complications on survival, longer-term life expectancy after hematopoietic not been assessed. PATIENTS AND METHODS We used standard methods to evaluate mortality, projected expectancy, causes death in a cohort 2,574 patients who survived without recurrence original disease for at least 5...