A5029255272- Immune Cell Function and Interaction
- Language Development and Disorders
- Phonetics and Phonology Research
- T-cell and B-cell Immunology
- Hearing Loss and Rehabilitation
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Natural Language Processing Techniques
- Reading and Literacy Development
- Monoclonal and Polyclonal Antibodies Research
- Linguistic Variation and Morphology
- Hearing Impairment and Communication
- Speech and dialogue systems
- Speech and Audio Processing
- Speech Recognition and Synthesis
- Hematopoietic Stem Cell Transplantation
- Employee Welfare and Language Studies
- Syntax, Semantics, Linguistic Variation
- Lexicography and Language Studies
- Glycosylation and Glycoproteins Research
- Second Language Acquisition and Learning
- Topic Modeling
- CAR-T cell therapy research
- Mast cells and histamine
- Cytokine Signaling Pathways and Interactions
University of Antwerp
2015-2024
Madigan Army Medical Center
2023
Rode Kruis-Vlaanderen
2023
University of Lethbridge
2020-2022
Hasselt University
2019
Otoconsult (Belgium)
2010
Japan External Trade Organization
1997
University of Washington
1982-1996
Fund for Scientific Research
1983-1994
Johns Hopkins University
1989-1994
Several soluble factors have recently been associated with the proliferation and differentiation of thymus-derived lymphocytes. One these present in medium conditioned by T cell mitogen-stimulated lymphocytes has ability to promote long-term culture normal antigen-specific cytotoxic cells. We report a method test for this proliferative stimulus form sensitive microassay based upon tritiated-thymidine incorporation continuous murine tumor-specific lines (CTLL). The requires microliter...
Leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) are multifunctional cytokines with many similar activities. LIF is structurally functionally related to another cytokine, Oncostatin M (OSM), that binds the high-affinity receptor but not low-affinity receptor. A complementary DNA was isolated encodes converting subunit of The converter conferred binding both OSM when expressed identical signal transducing IL-6 receptor, gp130. gp130 alone confers in COS-7 cells. This system resembles...
Interleukin-1 alpha and -1 beta (IL-1α IL-1β) are cytokines that participate in the regulation of immune responses, inflammatory reactions, hematopoiesis. A direct expression strategy was used to clone receptor for IL-1 from mouse T cells. The product cloned complementary DNA binds both IL-1α IL-1β a manner indistinguishable native cell receptor. extracellular, binding portion is 319 amino acids length composed three immunoglobulin-like domains. cytoplasmic 217 long.
Abstract By using as an experimental system the induction of growth a cloned, antigen:Ia-reactive helper T cell line by antigen receptor-specific monoclonal antibody, we demonstrated that requires two essential co-factors, exogenously produced IL 1 and endogenously 2. The primary role is in expression receptors on surface for 2, rather than promoting synthesis use clone-specific activating antibody at nanogram amounts to activate cloned should allow detailed characterization activation via...
The period of neutropenia after autologous bone marrow transplantation results in substantial morbidity and mortality. previous phase I-II clinical trials suggest that recombinant human granulocytemacrophage colony-stimulating factor (rhGM-CSF) may accelerate neutrophil recovery thereby reduce complications patients transplantation.
Human lymphocytes from elderly and young donors were cultured with phytohemagglutinin (PHA) or concanavalin A. Cultures old produced less T cell growth factor (TCGF) incorporated tritiated thymidine (3H-Tdr) than did similar cultures in the presence of either mitogen. Furthermore, response to TCGF was impaired. Thus, PHA-activated cells aged showed no increase incorporation when incubated exogenous human TCGF. In contrast, addition peripheral blood leukocytes younger individuals increased by...
The objective of this study was to define some causes the immunologic impairment characteristic systemic lupus erythematosus. Blood mononuclear cells from 19 patients were stimulated produce interleukin 1 and 2 compared with controls. A severe defect in both IL activity observed. low cytokine levels did not correlate clinical or serologic disease. These defects could be explained by concurrent corticosteroid therapy. There no correlation between a lower number OKT4+ observed these...
To isolate a stable tumor cell line capable of producing human interleukin 2 (IL-2; formerly referred to as T growth factor), 16 and B leukemia lines were screened for constitutive mitogen-stimulated IL-2 production. We found that the designated Jurkat-FHCRC produced > 200 U/ml activity after 24-h stimulation with mitogens. Peak mitogen-induced was in supernates harvested from cultures stimulated either 1% phytohemagglutinin or 20 microgram/ml concanavalin A. Addition fatty acid...
Monocytes are a subpopulation of peripheral blood leukocytes, which when appropriately activated by the regulatory hormones immune system, capable becoming macrophages--potent effector cells for response to tumors and parasites. A complementary DNA T lymphocyte-derived lymphokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), has been cloned, recombinant GM-CSF protein expressed in yeast purified homogeneity. This human stimulated monocytes vitro become cytotoxic malignant...
Interleukin 1 (IL-1) is a polypeptide hormone that acts as central mediator of inflammation. Since IL-1 action presumably mediated by specific cell surface receptor(s), we have characterized the binding this to cells. Purified human was labeled high activity with 125I, using Bolton-Hunter reagent. The protein binds specifically LBRM-33-1A5 (a murine T lymphoma line previously shown produce IL-2 in response phytohemagglutinin and IL-1) an affinity approximately 0.2-2 X 10(10)/M and, at...
Abstract Although it has been clearly established that glucocorticoids inhibit mitogen- or antigen-induced lymphocyte proliferation, the mechanism underlying this effect remained ill-defined. Recently, become evident T cell proliferation is mediated by a soluble growth factor (TCGF) released mitogen/antigen stimulated cells. Therefore seemed probable inhibitory effects of were manifested either at level TCGF production activated lymphocyte. We found differentiated cytolytic lymphocytes...
Monospecific cloned cytolytic T-lymphocyte lines have been created utilizing T-cell growth factor. The clones were found to retain their specificity after prolonged culture and monospecific function was demonstrated by subcloning procedures. Thus, detailed studies of the phenotypic functional characteristics monospecific, homogeneous, T lymphocytes will now be possible.
Both IL-la and IL-18 are initially translated as approximately M , 30,000 polypeptides processed to M, 17,500 prior to, or during, release from macrophages.The current study utilizes an in vitro transcription-translation system produce these four forms of IL-1 directly cloned cDNAs, order investigate the relative receptor binding biological activities proteins.The data show that initial translation product mRNA must be bind hence express activity.By contrast, ILla can without further...
Murine spleen cells activated by concanavalin A (Con A) in culture produce a class of lymphokine molecules which possess biological activity number lymphocyte response assays. Lymphokines with mol wt 30,000, as estimated from gel filtration studies, can be resolved into two components differ charge, isoelectric point (pI) values 4.3 and 4.9, respectively. Both stimulate (a) the growth established T-cell lines culture, (b) proliferation thymocytes presence Con under conditions where alone is...
Human granulocyte/macrophage colony-stimulating factor (GM-CSF) is a glycoprotein that essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes macrophages. In this report we have used mouse GM-CSF cDNA clone to isolate human clones from libraries made HUT-102 messenger RNA mitogen-stimulated T-lymphocyte RNA. The contained single open-reading frame encoding protein 144 amino acids with predicted molecular mass 16,293 daltons showed 69%...
The gene product of the steel locus mouse represents a growth factor for murine mast cells and ligand c-kit proto-oncogene receptor, member tyrosine kinase receptor class oncogenes (for review, see O. N. Witte. 1990. Cell 63:5). We have studied effect human recombinant stem cell (rhSCF) on release inflammatory mediators from skin peripheral blood basophils compared its activity to that rhIL-3, rhSCF (1 ng/ml 1 microgram/ml) activated histamine PGD2 isolated skin. Analysis by digital video...