A5043798026- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Lung Cancer Research Studies
- Cancer therapeutics and mechanisms
- Immune Response and Inflammation
- Neuroendocrine Tumor Research Advances
- Immune Cell Function and Interaction
- Ion channel regulation and function
- Signaling Pathways in Disease
- Immunotherapy and Immune Responses
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Erythrocyte Function and Pathophysiology
- CAR-T cell therapy research
- Glioma Diagnosis and Treatment
- Synthesis and Characterization of Heterocyclic Compounds
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Mitochondrial Function and Pathology
- Neurofibromatosis and Schwannoma Cases
- Viral Infectious Diseases and Gene Expression in Insects
- Chemokine receptors and signaling
Istituto Giannina Gaslini
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2024
Institute of Cancer Research
2014
ANT Foundation Italy Onlus
2006
University of Bari Aldo Moro
2006
University of Genoa
1993-2004
Boston Children's Hospital
1990-1997
University of Mississippi Medical Center
1987
University of Cagliari
1985
Abstract The human microbiome is an integral component of the body and a co-determinant several health conditions 1,2 . However, extent to which interpersonal relations shape individual genetic makeup its transmission within across populations remains largely unknown 3,4 Here, capitalizing on more than 9,700 metagenomes computational strain-level profiling, we detected extensive bacterial strain sharing individuals (more 10 million instances) with distinct mother-to-infant, intra-household...
In this study, in an attempt to identify neuroblastoma-associated surface antigens, we generated mAbs against the ACN neuroblastoma cell line. A mAb was selected (5B14) that reacted with all lines analyzed and allowed detection of tumor infiltrates bone marrow aspirates from patients. cytofluorimetric analysis, unlike anti-disialoganglioside mAb, 5B14 did not display reactivity normal hematopoietic precursors, thus representing a highly specific marker for identifying cells. Molecular...
Abstract In the present study, we assessed susceptibility of freshly isolated neuroblastoma cells to killing mediated by normal human natural killer (NK) and analyzed receptor–ligand interactions that regulate this event. We show neuroblasts, similar cell lines, involves NKp46 NKp30 (natural cytotoxicity receptors). However, neuroblasts were generally more resistant NK-mediated lysis than conventional lines. Moreover, a significant heterogeneity in existed among neuroblastomas derived from...
Abstract In this study, we show that neuroblastoma (NB) cell conditioning affects the chemokine receptor repertoire of human resting NK cells. particular, NB cells upregulated expression CXCR4 and CXCR3 in all downregulated CX3CR1 CD56dim subset. On contrary, CXCR1 CCR7 remained unaltered. The phenomenon was dependent on release by TGF-β1, rTGF-β1 induced a identical to NB-conditioned immune modulatory role TGF-β1 appears be dose because low amounts cytokine were sufficient modulate...
To evaluate the hypothesis that detection of neuroblastoma mRNAs by reverse transcriptase quantitative polymerase chain reaction (RTqPCR) in peripheral blood (PB) and bone marrow aspirates (BM) from children with stage 4 are clinically useful biomarkers risk.
The prognosis of high-risk neuroblastoma (NB) remains poor, although immunotherapies with anti-GD2 antibodies have been reported to provide some benefit. Immunotherapies can be associated an IFNγ storm that induces in tumor cells the "adaptive immune resistance" characterized by de-novo expression Programmed Death Ligands (PD-Ls). Tumor also constitutively express PD-Ls response oncogenic signaling. Here, we analyze constitutive and inducible surface NB cells. We show virtually all HLA class...
HIF1A (Hypoxia-Inducible-Factor 1A) expression in solid tumors is relevant to establish resistance therapeutic approaches. The use of compounds direct against hypoxia signaling and does not show clinical efficiency because changeable oxygen concentrations tumor areas. identification targets expressed both normoxia HIF1A/hypoxia signatures might meliorate the prognostic stratification successes patients with high-risk tumors. In this study, we conducted a combined analysis RNA DNA methylation...
Bortezomib is a selective and reversible inhibitor of the 26S proteasome that shows potent antitumor activity in vitro vivo against several human cancers adulthood. No data are available on bortezomib pediatric neuroblastoma.Ten neuroblastoma cell lines suspensions primary cells from three patients were tested for sensitivity to bortezomib. Colony formation, proliferation, cycle progression, apoptosis evaluated by clonogenic assay measuring 3H-thymidine incorporation, bromodeoxyuridine...
Purpose Malignant cells are genetically unstable and prone to develop chemotherapy resistance, whereas tumor vasculature is usually of host origin stable. Tumor endothelial microvessels attract interest as therapeutic targets, but their genetic instability would curtail such approach. Here, we have investigated the in human neuroblastoma (NB). Materials Methods Paraffin-embedded tissue sections from 10 MYCN-amplified tumors (six stage 4, three 3, one 1) were studied. Endothelial (ECs)...
Neuroblastoma (NB) represents the most frequent and aggressive form of extracranial solid tumor infants. Nucleolin (NCL) is a protein overexpressed partially localized on cell surface cells adult cancers. Little known about NCL pediatric tumors nothing reported NB.NB lines, Schwannian stroma-poor NB bone marrow (BM)-infiltrating were evaluated for expression by Flow Cytometry, Imaging Cytometry Immunohistochemistry analyses. The cytotoxic activity doxorubicin (DXR)-loaded nanocarriers...
To assess whether the expression of B7-H3 surface molecule could improve differential diagnosis small cell round tumours.One hundred and one well-characterized paraffin-embedded tumours, stored in pathology archive Gaslini Institute, were immunohistochemically analysed with 5B14 monoclonal antibody, which recognizes B7-H3. All lymphoblastic lymphomas blastematous component Wilms' tumours completely negative a few Ewing's sarcoma Burkitt's lymphoma specimens showed focal positivity, whereas...
Different plasma and urinary parameters have been tested as valuable prognostic markers for children with neuroblastoma (NB), but conclusive results from multivariate analyses are still lacking. Samples collected at diagnosis 505 patients diagnosed in Italy between June 1994 November 2010 were analyzed the Italian reference laboratory according to standard methodologies. Patient clinical data retrieved NB Registry. For statistical analysis, grouped stage, age, MYCN status, outcome....
Despite intensive treatment, 50% of children with high-risk neuroblastoma (HR-NB) succumb to their disease. Progression through current trials evaluating the efficacy new treatments for HR disease usually depends on an inadequate response induction chemotherapy, assessed using imaging modalities. In this study, we sought identify circulating biomarkers that might be detected in a simple blood sample predict patient chemotherapy. Since exosomes released by tumor cells can drive growth and...
The human microbiome seeding starts at birth, when pioneer microbes are acquired mainly from the mother. Mode of delivery, antibiotic prophylaxis, and feeding method have been studied as modulators mother-to-infant transmission, but other key influencing factors like modern westernized lifestyles with high hygienization, high-calorie diets, urban settings, compared non-westernized not investigated yet. In this study, we explored mother-infant sharing characterized uncharacterized members via...
Abstract Tyrosine kinase inhibitors (TKIs) are used in the clinical management of hematological neoplasms. Moreover, solid tumors such as stage 4 neuroblastomas (NB), imatinib showed benefits that might depend on both on-target and immunological off-target effects. We investigated effects nilotinib human NK cells, monocytes, macrophages. High numbers monocytes died upon exposure to TKI concentrations similar those achieved patients. Conversely, cells were highly resistant cytotoxic effect,...
Anti-PD-1 or anti-PD-L1 blocking monoclonal antibodies (mAbs) have shown potent anti-tumor effects in adult cancer patients and clinical studies recently been started pediatric cancers, including high-risk/relapsing neuroblastoma (NB). Therefore, we studied the of anti-PD-1/PD-L1 mAbs two syngeneic models disseminated NB generated by injection either Neuro2a NXS2 cells, which express PD-L1. In addition, tested combination these agents with immune-enhancing cytokine IL-21, Ecto-NTPDase...
Abstract Neuroblastoma (NB) tumor substantially contributes to childhood cancer mortality. The design of novel drugs targeted specific molecular alterations becomes mandatory, especially for high‐risk patients burdened by chemoresistant relapse. dysregulated expression MYCN , ALK and LIN28B the diminished levels miR‐34a let‐7b are oncogenic in NB. Due ability miRNA‐mimics recover suppression functions miRNAs underexpressed into cells, safe efficient nanocarriers selectively NB cells tested...
Introduction B7-H3 is a potential target for pediatric cancers, including neuroblastoma (NB). Vobramitamab duocarmazine (also referred to as MGC018 and herein vobra duo) an investigational duocarmycin-based antibody-drug conjugate (ADC) directed against the antigen. It composed of anti-B7-H3 humanized IgG1/kappa monoclonal antibody chemically conjugated through cleavable valine-citrulline linker du ocarmycin-hydroxy b enzamide zaindole (vc-seco-DUBA). Vobra duo has shown preliminary clinical...
Abstract: We studied the constitutive and interferon (IFN)‐γ‐induced expression of HLA class I antigen heavy chain, β 2 ‐microglobulin (β m), TAP‐1, TAP‐2 tapasin in a panel eleven neuroblastoma cell lines. Surface antigens was low eight out lines bearing MYC‐N amplification and/or 1p deletion, while two three lacking these genetic alterations showed normal expression. IFN‐γ treatment restored surface all Eight 11 did not express TAP‐1 mRNA them also lacked mRNA. m barely detectable or...
Metastases in the bone marrow (BM) are grim prognostic factors patients with neuroblastoma (NB). In spite of extensive analysis primary tumor cells from high- and low-risk NB patients, a characterization freshly isolated BM-infiltrating metastatic is still lacking. Our aim was to identify proteins specifically expressed by cells, that may be relevant for therapeutic purposes. Sixty-six Italian children over 18 months age, diagnosed stage 4 NB, were included study. Metastatic patients' BM...
Neuroblastoma (NB) is the most common extra-cranial solid tumor of pediatric age. The prognosis for high-risk NB patients remains poor, and new treatment strategies are desirable. olive leaf extract (OLE) constituted by phenolic compounds, whose health beneficial effects were reported. Here, anti-tumor OLE investigated in vitro on a panel cell lines terms (i) reduction viability; (ii) inhibition proliferation through cycle arrest; (iii) induction apoptosis; (iv) migration. Furthermore,...
GD2 ganglioside, a known specific marker for neuroblastoma (NB), exists in different lipoforms, including C18 and C20, which are distinguished by the length of their fatty acid chains. C20 lipoforms can be simultaneously measured liquid chromatography-tandem mass spectrometry (LC-MS/MS). We evaluated diagnostic prognostic performance circulating levels children with NB. Thirty microliters peripheral blood (PB) plasma samples from 83 NB at diagnosis age-matched healthy controls were analyzed...