A5023069361- Adenosine and Purinergic Signaling
- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Migraine and Headache Studies
- Alcoholism and Thiamine Deficiency
- Neuroscience of respiration and sleep
- Nanoplatforms for cancer theranostics
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Neuroblastoma Research and Treatments
- Virus-based gene therapy research
- Brain Metastases and Treatment
- Amino Acid Enzymes and Metabolism
- Glioma Diagnosis and Treatment
- Nicotinic Acetylcholine Receptors Study
- Italian Literature and Culture
- Heat shock proteins research
- Salivary Gland Tumors Diagnosis and Treatment
- HIV/AIDS oral health manifestations
- Pharmacological Effects and Toxicity Studies
- Adolescent and Pediatric Healthcare
- Click Chemistry and Applications
- Japanese History and Culture
- Plant and Fungal Interactions Research
University of Verona
2021
Istituto delle Scienze Neurologiche di Bologna
2018-2020
Ospedale Bellaria
2020
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2019
University of Bologna
2010-2018
University of Ferrara
2014-2016
Scuola Internazionale Superiore di Studi Avanzati
2012-2013
Italian Institute of Technology
2012
The P2X7 receptor (P2X7R) is a known and powerful activator of the NOD-like (NLR)P3 inflammasome; however, underlying pathways are poorly understood. Thus, we investigated molecular mechanisms involved. effect P2X7R expression activation on NLRP3 recruitment was by Western blot, RT-PCR, coimmunoprecipitation, confocal microscopy in microglial mouse cell lines selected for reduced primary cells from P2X7R-/- C57BL/6 mice. We show here that ATP (EC50 = 1 mM) or benzoyl-ATP 300 μM)...
Abstract An early and accurate in vivo diagnosis of rapidly progressive dementia remains challenging, despite its critical importance for the outcome treatable forms, formulation prognosis. Real-Time Quaking-Induced Conversion (RT-QuIC) is an vitro assay that, first time, specifically discriminates patients with prion disease. Here, using cerebrospinal fluid (CSF) samples from 239 definite or probable disease 100 a alternative diagnosis, we compared performance (PQ-CSF) second generation...
The ATP receptor P2X7 (P2X7R or P2RX7) has a key role in inflammation and immunity, but its possible roles cancer are not firmly established. In the present study, we investigated effect of host genetic deletion P2X7R mouse on growth B16 melanoma CT26 colon carcinoma cells. Tumor size metastatic dissemination were assessed by vivo calliper luciferase luminescence emission measurements along with postmortem examination. P2X7R-deficient mice, tumor spreading accelerated strongly, compared...
The differential diagnosis of Creutzfeldt-Jakob disease (CJD) from other, sometimes treatable, neurological disorders is challenging, owing to the wide phenotypic heterogeneity disease. Real-time quaking-induced prion conversion (RT-QuIC) a novel ultrasensitive in vitro assay, which, at variance with surrogate neurodegenerative biomarker assays, specifically targets pathological protein (PrPSc). In studies conducted date CJD, cerebrospinal fluid (CSF) RT-QuIC showed good diagnostic...
Latent changes in trigeminal ganglion structure and function resembling inflammatory conditions may predispose to acute attacks of migraine pain. Here, we investigated whether, sensory ganglia, cytokines such as TNFα might contribute a local phenotype transgenic knock-in (KI) mouse model familial hemiplegic type-1 (FHM-1). To this end, macrophage occurrence cytokine expression ganglia were compared between wild type (WT) R192Q mutant CaV2.1 Ca2+ channel (R192Q KI) mice, genetic FHM-1....
Abstract Prion real‐time quaking‐induced conversion (RT‐QuIC) is an ultrasensitive assay detecting pathological aggregates of misfolded prion protein in biospecimens. We studied 71 punch biopsy skin samples 35 patients with Creutzfeldt–Jakob disease (CJD), including five assessed vitam. The results confirmed the high value RT‐QuIC for CJD diagnosis (89% sensitivity and 100% specificity) support its use clinical practice. Preliminary data based on a limited number cases suggest that...
Abstract Lymphocyte growth and differentiation are modulated by extracellular nucleotides P2 receptors. We previously showed that the P2X7 receptor (P2X7R or P2RX7) is overexpressed in circulating lymphocytes from chronic lymphocytic leukemia (CLL) patients. In present study we investigated P2X7R/NLRP3 inflammasome axis a cohort of 23 CLL P2X7R, ASC NLRP3 were Western blot, PCR transfection techniques. P2X7R was correlated with chromosome 12 trisomy mRNA protein also overexpressed. On...
Objective To assess whether the involvement of peripheral nervous system (PNS) belongs to phenotypic spectrum sporadic Creutzfeldt-Jakob disease (sCJD). Methods We examined medical records 117 sCJDVV2 (ataxic type), 65 sCJDMV2K (kuru-plaque type) and 121 sCJDMM(V)1 (myoclonic subjects for clinical symptoms, objective signs neurophysiological data. reviewed two diagnostic nerve biopsies looked abnormal prion protein (PrP Sc ) by western blotting real-time quaking-induced conversion (RT-QuIC)...
Abstract Background Enhanced activity of trigeminal ganglion neurons is thought to underlie neuronal sensitization facilitating the onset chronic pain attacks, including migraine. Recurrent headache attacks might establish a neuroinflammatory profile contributing hypersensitive phenotype. Since it difficult study this process in vivo , we investigated functional crosstalk between macrophages and sensory primary cultures from ganglia wild-type (WT) or knock-in (KI) mice expressing Cacna1a...
The aim of the present study was to assess whether exposure a sinusoidal extremely low frequency magnetic field (ELF-MF; 50 Hz, 1 mT) can affect proliferation and differentiation in human neuroblastoma cell line BE(2)C, which is representative high risk neuroblastomas. Cells were subjected ELF-MF presence or absence neuronal differentiating agent (all-trans-retinoic acid, ATRA) for 24-72 h. In each experiment, ELF-MF-exposed samples compared sham-exposed samples. exposed combined with...
Aims The aim of this study was to describe the regional profiles microglial activation in sporadic Creutzfeldt‐Jakob disease ( sCJD ) subtypes and analyse influence prion strain, duration codon 129 genotype. Methods We studied amount/severity distribution activated microglia, protease‐resistant protein (PrP Sc spongiform change, astrogliosis eight regions 57 brains, representative entire spectrum subtypes. Results In each individual subtype, extent microgliosis significantly correlated with...
<div>Abstract<p>The ATP receptor P2X7 (P2X7R or P2RX7) has a key role in inflammation and immunity, but its possible roles cancer are not firmly established. In the present study, we investigated effect of host genetic deletion P2X7R mouse on growth B16 melanoma CT26 colon carcinoma cells. Tumor size metastatic dissemination were assessed by <i>in vivo</i> calliper luciferase luminescence emission measurements along with postmortem examination. P2X7R-deficient mice,...
<p>Figure S2. Core necrosis in B16 melanomas. Representative images of central melanoma masses P2X7R-KO (A and B) or wt (C D) mice. Specimens were fixed stained with haematoxylin-eosin as described Materials Methods.</p>
<p>Legends to Supplementary Figures</p>
<p>Figure S1. Characterization of P2X7R shRNA B16 cells. A, densitometry protein expression. B, representative Western blot. C, Fura-2 measurement Benzoyl ATP (BzATP)-triggered intracellular Ca2+ increase. D, Averages increases in wt and P2X7R-silenced Data are averages + SEM, n = 5. ***, p < 0.001. E, rate proliferation cell cultures. Intracellular changes rates were measured as described Adinolfi et al., 2005, Mol Biol Cell 16:3260-3272.</p>
<p>Figure S3. Effect of P2X7R deletion on VEGF release. A, in vitro release from wt or P2X7R-silenced B16 cells. B, intra-tumor content B16-wt tumors grown P2X7R-KO mice. was measured by ELISA. * p < 0.05 for shRNA vs B16-wt. P2X7R-wt + B16-wt; {section sign}{section sign} 0.01 C-F, staining (C and D) (E F) Specimens were fixed stained with the anti-VEGF Ab as described Materials Methods.</p>
<p>SMovie S4. Migration of wt N13 microglial cells from both sides a wounded monolayer after scratching with plastic pipette tip.</p>