A5057896638- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Extracellular vesicles in disease
- RNA Research and Splicing
- Cancer Cells and Metastasis
- interferon and immune responses
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Acute Myeloid Leukemia Research
- Cancer, Lipids, and Metabolism
- Lung Cancer Treatments and Mutations
- HER2/EGFR in Cancer Research
- Protein Degradation and Inhibitors
- Inflammatory mediators and NSAID effects
- Immune cells in cancer
- Cancer Mechanisms and Therapy
- Cancer therapeutics and mechanisms
- Ferroptosis and cancer prognosis
- Eicosanoids and Hypertension Pharmacology
- Immunotherapy and Immune Responses
- Lung Cancer Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Liver Disease Diagnosis and Treatment
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
Ospedale G.B. Morgagni - L.Pierantoni
2023
Children's Hospital of Los Angeles
2015
Children's Center
2015
University of Southern California
2015
The University of Texas MD Anderson Cancer Center
2015
Gene Therapy Laboratory
2014
Istituto Oncologico Romagnolo
2005-2007
University of Bologna
2004
How exosomic microRNAs (miRNAs) contribute to the development of drug resistance in context tumor microenvironment has not been previously described neuroblastoma (NBL). Coculture experiments were performed assess transfer miR-21 from NBL cells human monocytes and miR-155 cells. Luciferase reporter assays targeting TERF1 Tumor growth was measured xenografts treated with Cisplatin peritumoral (n = 6 mice per group) CD163, miR-155, levels assessed 20 primary tissues by Human Exon Arrays...
Inactivation of mismatch repair (MMR) is the cause common cancer predisposition disorder Lynch syndrome (LS), also known as hereditary nonpolyposis colorectal (HNPCC), well 10–40% sporadic colorectal, endometrial, ovarian, gastric, and urothelial cancers. Elevated mutation rates (mutator phenotype), including simple repeat instability [microsatellite (MSI)] are a signature MMR defects. MicroRNAs (miRs) have been implicated in control critical cellular pathways involved development cancer....
Purpose: The oncogenic miR-155 is upregulated in many human cancers, and its expression increased more aggressive therapy-resistant tumors, but the molecular mechanisms underlying miR-155-induced therapy resistance are not fully understood. main objectives of this study were to determine role chemotherapy evaluate anti-miR-155 treatment chemosensitize tumors.Experimental Design: We performed vitro studies on cell lines investigate resistance. To assess effects inhibition chemoresistance, we...
<h3>Objective</h3> MicroRNA (miRNA) expression profile can be used as prognostic marker for human cancers. We aim to explore the significance of miRNAs in colorectal cancer (CRC) metastasis. <h3>Design</h3> performed miRNA microarrays using primary CRC tissues from patients with and without metastasis, validated selected candidates 85 samples by quantitative real-time PCR (qRT-PCR). tested metastatic activity identified targets prediction algorithms, qRT-PCR, western blot luciferase assays....
Ultraconserved regions (UCRs) have been shown to originate non-coding RNA transcripts (T-UCRs) that different expression profiles and play functional roles in the pathophysiology of multiple cancers. The relevance these functions pathogenesis bladder cancer (BlCa) is speculative. To elucidate this relevance, we first used genome-wide profiling evaluate T-UCRs BlCa tissues. Analysis two datasets comprising normal tissues specimens with a custom T-UCR microarray identified ultraconserved (uc.)...
Abstract Sorafenib is a multikinase inhibitor that has shown promising therapeutic results in different tumor histotypes, both as single agent or combination with other treatments. We analyzed the vitro activity of sorafenib pancreatic cancer, one most lethal and chemo‐radio‐resistant tumors, using four human cancer cell lines (t3m4, Capan 1, 2, MiaPaca 2), characterized by K‐ras gene status RAF/MEK/ERK profile. exerted strong anti‐proliferative effect independently RAS/RAF/MEK/ERK induced...
Approximately 7% of cancers arising in children and 1% those adults are soft tissue sarcomas (STS). Of these malignancies, rhabdomyosarcoma (RMS) is the most common. RMS survival rates using current therapeutic protocols have remained largely unchanged past decade. Thus, it imperative that main molecular drivers tumorigenesis defined so more precise, effective, less toxic therapies can be designed. Curcumin, a common herbal supplement derived from plants Curcuma longa species, has an...
Abstract Tumor dissemination is a key event in tumor progression. During this event, main role played by circulating cells (CTCs), immune cells, and their interaction. How the system supports survival proliferation of CTCs not fully elucidated. In study we established an in-vitro co-culture consisting from same patient, which increased success rate establishment CTC-derived long-term cell cultures. system, characterized successful co-cultures signals they exchange with cancer including...
Abstract Introduction The aim of the study was to evaluate activity a combination doxorubicin (Dox), paclitaxel (Pacl) and 5-fluorouracil (5-FU), define most effective schedule, investigate mechanisms action in human breast cancer cells. Methods performed on MCF-7 BRC-230 cell lines. cytotoxic evaluated by sulphorhodamine B assay type drug interaction assessed median effect principle. Cell cycle perturbation apoptosis were flow cytometry, apoptosis-related marker (p53, bcl-2, bax, p21),...
Abstract Studies performed in different experimental and clinical settings have shown that Docetaxel (Doc) is effective a wide range of tumors it exerts its activity through multiple mechanisms action. However, the sequence events induced by Doc which leads to cell death still not fully understood. Moreover, completely clear how induces mitotic catastrophe whether this process an end event or followed apoptosis necrosis. We investigated triggers hormone‐refractory prostate cancer cells...
The transcribed ultraconserved regions (T-UCRs) encode long non-coding RNAs implicated in human carcinogenesis. Their mechanisms of action and the factors regulating their expression cancers are poorly understood. Here we show that high uc.339 correlates with lower survival 210 non-small cell lung cancer (NSCLC) patients. We provide evidence from lines primary samples TP53 directly regulates uc.339. find is upregulated archival NSCLC samples, functioning as a decoy RNA for miR-339-3p,...
This study aimed to isolate and characterize stem/progenitor cells, starting from normal airway epithelia, obtained human adults.Cultures of multicellular spheroids were lung tissue specimens after mechanical enzymatic digestion. Tissue-specific markers detected on their cells by immunohistochemical immunofluorescent techniques. Ultrastructural morphology the (termed as bronchospheres) was evaluated electron microscopy, gene expression analysis performed reverse transcription-polymerase...
The identification of predictive factors for treatment pancreatic cancer (PC) is an unmet clinical need. In the present work, we analyzed blood-derived extracellular vesicles (EVs) from patients with advanced PC in order to find a molecular signature response therapy. We samples 21 PC, all receiving first-line gemcitabine + nab-paclitaxel. Isolated EVs have been analyzed, and results laboratory matched data investigate possible factors. EV concentration size were similar between responder...
Breast cancer (BC) is the most diagnosed carcinoma and leading cause of death in female over 100 countries. Thanks to advance therapeutic strategies, patients' survival has improved. However, lack response treatments drug resistance are still a main concern, demanding for new approaches, particular advanced stages disease. Androgen receptor (AR) gaining increasing interest as fourth targetable BC, however, its regulation BC cells poorly understood. MicroRNAs (miRNAs) regulate gene expression...
Retinoblastoma (Rb) is the most common ocular paediatric malignancy and caused by a mutation of two alleles tumor suppressor gene, RB1. The microenvironment (TME) represents complex system whose function not yet well defined where microvesicles, such as exosomes, play key role in intercellular communication. Micro-RNAs (mRNAs) have emerged important modifiers biological mechanisms involved cancer been able to regulate progression.Co-culture monocytes with retinoblastoma cell lines, showed...