A5080486262- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
- Nuclear Structure and Function
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- PI3K/AKT/mTOR signaling in cancer
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Sarcoma Diagnosis and Treatment
- Muscle Physiology and Disorders
- Erythrocyte Function and Pathophysiology
- Metabolism, Diabetes, and Cancer
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Cytokine Signaling Pathways and Interactions
- Acute Myeloid Leukemia Research
- Ocular Surface and Contact Lens
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Advanced Drug Delivery Systems
- Cardiomyopathy and Myosin Studies
- Rheumatoid Arthritis Research and Therapies
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
Istituto Ortopedico Rizzoli
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2024-2025
Orthopedic Institute
2013-2017
Institute of Cell Biology and Neurobiology
1998-2009
University of Ferrara
1994-2009
University of Bologna
1995-2009
University of Rome Tor Vergata
2009
University of Modena and Reggio Emilia
2008
University of Brescia
1995
Indiana University – Purdue University Indianapolis
1995
Interleukin 1 (IL-1) is one of the most potent stimulators bone resorption. However, early biochemical events elicited by IL-1 receptor binding are not fully understood. Here we show that in human osteosarcoma SaOS-2 cells treatment with alpha able to evoke a rapid and transient increase nuclear phospholipase C (PLC) activity. A parallel decrease phosphatidylinositol monophosphate bisphosphate observed. All these strictly confined compartment without affecting cytoplasmatic inositol lipid...
Akt/PKB is a central activator of multiple signaling pathways coupled with large number stimuli. Although both localization and activity Akt in the nuclear compartment are well-documented, most substrates identified so far located cytoplasm, while have remained elusive. A proteomic-based search for was undertaken, exploiting 2D-electrophoresis/MS combination an anti-Akt phosphosubstrate antibody. This analysis indicated lamin A/C as putative substrate C2C12 cells. In vitro phosphorylation...
Approximately 7% of cancers arising in children and 1% those adults are soft tissue sarcomas (STS). Of these malignancies, rhabdomyosarcoma (RMS) is the most common. RMS survival rates using current therapeutic protocols have remained largely unchanged past decade. Thus, it imperative that main molecular drivers tumorigenesis defined so more precise, effective, less toxic therapies can be designed. Curcumin, a common herbal supplement derived from plants Curcuma longa species, has an...
Ankrd2, a mechanoresponsive protein primarily studied in muscle physiology, is emerging as player cancer progression. This study investigates the functional role of Ankrd2 osteosarcoma cells, revealing its critical involvement cell proliferation and response to chemotherapeutic drugs. We showed that knockdown impairs activation PI3K/Akt ERK1/2 pathways, reduces levels cycle regulators including cyclin D1 B, counteracts expression nuclear lamin A disrupting morphology DNA integrity....
Functional foods, thanks to their basic nutritional properties, can have physiological benefits and alleviate the symptoms of many chronic diseases. They contain active components deriving either from plant or animal sources, they show anti-inflammatory, cardiotonic, antioxidant pharmacological activities that could be useful in preventing oxidative damage inflammatory processes a variety disorders. There is evidence vitro, vivo, clinical observational studies some compounds significant...
Abstract Background Beyond its possible correlation with stemness of tumor cells, CD133/prominin1 is considered an important marker in breast cancer, since it correlates size, metastasis and clinical stage triple-negative cancers (TNBC), to date the highest risk neoplasia. Methods To study between levels CD133 expression biology breast-derived low high cell subpopulations isolated from triple negative MDA-MB-231 cells were compared terms malignant properties protein expression. Results High...
Interleukin 1 (IL‐1) delivers a stimulatory signal which increases the expression of set genes by modulating transcription factor NF‐κB. The IL‐1 receptors are transmembrane glycoproteins lack catalytic domain. C‐terminal portion type I receptor (IL‐1RI) is essential for signalling and dependent activation This contains putative phosphatidylinositol 3‐kinase (PI 3‐kinase) binding domain (Tyr‐E‐X‐Met), highly conserved between human, mouse chicken sequences, as well related cytoplasmic...
Phospholipase C beta(1) (PLCbeta(1)) signaling in both cell proliferation and differentiation has been largely investigated, but its role myoblast is still unclear. The C2C12 myogenic line used this study order to find out the of two subtypes PLCbeta(1), i.e., a b process. proliferate response mitogens upon mitogen withdrawal differentiates into multinucleated myotubes. We found that skeletal muscle cells characterized by marked increase amount nuclear PLCbeta(1)a PLCbeta(1)b. Indeed,...
Activation of Akt-mediated signaling pathways is crucial for survival, differentiation, and regeneration muscle cells. A proteomic-based search novel substrates Akt was therefore undertaken in C(2)C(12) murine cells exploiting protein characterization databases combination with an anti-phospho-Akt substrate antibody. Scansite database predicted Ankrd2 (Ankyrin repeat domain 2, also known as ARPP) a Akt. In vitro vivo studies confirmed that phosphorylates at Ser-99. Moreover, by kinase assay...
The AKT/PKB kinase is essential for cell survival, proliferation, and differentiation; however, aberrant AKT activation leads to the aggressiveness drug resistance of many human neoplasias. In acute promyelocytic leukemia line NB4, nuclear activity increases during all-trans retinoic acid (ATRA)-mediated differentiation. As associated with differentiation, we sought identify substrates that were phosphorylated after ATRA treatment. A proteomics-based search in ATRA-treated NB4 cells was...
The advent of next generation sequencing (NGS) has fostered a shift in basic analytic strategies gene expression analysis diverse pathologies for the purposes research, pharmacology, and personalized medicine. What was once highly focused research on individual signaling pathways or pathway members has, from time arrays, become global that aided identifying novel interactions, discovery new therapeutic targets, establishment disease-associated profiles assessing progression, stratification,...
Our main goal in this study was to investigate the role of phospholipase C (PLC) beta(1) and PLCgamma(1) skeletal muscle differentiation existence potential downstream targets their signaling activity. To examine whether PLC can modulate expression cyclin D3, a target PLCbeta(1) erythroleukemia cells, we transfected C2C12 cells with vectors containing or cDNA small interfering RNAs from regions gene followed myogenic well-established cell system. Intriguingly, overexpressed were able mimic...
Bone morphogenetic protein 2 (BMP-2) is a critical growth factor that directs osteoblast differentiation and bone formation. Phosphoinositide-phospholipase Cβ 1 (PLCβ1) plays crucial role in the initiation of genetic program responsible for muscle differentiation. Differentiation C2C12 mouse myoblasts response to insulin stimulation characterized by marked increase nuclear PLCβ1. Here, function PLCβ1 osteogenic was investigated. Briefly, cells treated with BMP-2 we assist remarkable mRNA...
ABSTRACT Although interleukin 1 (IL-1) functions have been extensively characterized, the mechanisms by which IL-1 signals are transduced from plasma membrane to nucleus less known. Recent evidence indicates that phosphatidylinositol 3-kinase (PI3-kinase) could be activated a direct association with receptor. In this study we analyzed effects of on intracellular distribution PI3-kinase in wild-type Saos-2 human osteosarcoma cells, and cell clones overexpressing type I receptor (IL-1RI)....