A5043457145- Galectins and Cancer Biology
- Thyroid Cancer Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Cutaneous Melanoma Detection and Management
- Cancer Mechanisms and Therapy
- Head and Neck Anomalies
- Melanoma and MAPK Pathways
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Neuroblastoma Research and Treatments
- Thyroid and Parathyroid Surgery
- Wnt/β-catenin signaling in development and cancer
- Vascular Tumors and Angiosarcomas
- Advanced Chemical Sensor Technologies
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Sarcoma Diagnosis and Treatment
- Protease and Inhibitor Mechanisms
- Salivary Gland Tumors Diagnosis and Treatment
- Biochemical Analysis and Sensing Techniques
- Lymphoma Diagnosis and Treatment
CTO Andrea Alesini
2013-2024
Sapienza University of Rome
2005-2024
Karolinska University Hospital
2009-2021
Azienda Ospedaliera Sant'Andrea
2003-2020
Ministero della Salute
2018-2019
Karolinska Development (Sweden)
2018
Alder Hey Children's NHS Foundation Trust
2010
Bambino Gesù Children's Hospital
2010
Istituto Centrale per la Ricerca Scientifica e Tecnologica Applicata al Mare
2009
Politecnico di Milano
2009
Abstract The insulin-like growth factor-1 receptor (IGF-1R) plays a pivotal role in transformation, growth, and survival of malignant cells, has emerged as general promising target for cancer treatment. However, no fully selective IGF-1R inhibitors have thus far been found. This is explained by the fact that highly homologous to insulin receptor, coinhibition which may cause diabetic response. receptors are both tyrosine kinases, their ATP binding sites identical, implying cannot...
CD44 is implicated in the regulation of tumor growth and metastasis but mechanism by which expression different isoforms determines rate primary secondary remains unclear. In present study we use a human melanoma transfected with wild-type mutant forms to determine functional property molecule critical influencing behavior. We show that isoform binds hyaluronic acid augments rapidity formation cells vivo, whereas mutant, does not mediate cell attachment hyaluronate, fails do so. The...
CD44-mediated cell adhesion to hyaluronate is controlled by mechanisms which are poorly understood. In the present work we examine role of N-linked glycosylation and Ser-Gly motifs in regulating CD44-hyaluronate interaction. Our results show that treatment a panel human lines constitutively express CD44 with inhibitor tunicamycin loss attachment these cells hyaluronate-coated substrate. contrast, same deoxymannojirimycin, inhibits conversion high mannose oligosaccharides complex...
Background Galectin-3 (Gal-3) is an anti-apoptotic molecule involved in thyroid cells transformation. It specifically overexpressed tumour and currently used as a preoperative diagnostic marker of malignancy. Gal-3 expression downregulated by wt-p53 at the transcriptional level. In well-differentiated carcinomas (WDTCs) there unexplained paradoxical concomitant wt-p53. HIPK2 co-regulator different transcription factors, modulates basic cellular processes mainly through activation Since we...
The hyaluronan (HA)-binding function (lectin function) of the leukocyte homing receptor, CD44, is tightly regulated. Herein we address possible mechanisms that regulate CD44 isoform-specific HA binding. Binding studies with melanoma transfectants expressing CD44H, CD44E, or soluble immunoglobulin fusions CD44H and CD44E (CD44H-Rg, CD44E-Rg) showed although both isoforms can bind HA, binds more efficiently than CD44E. Using CD44-Rg fusion proteins show variably spliced exons in V8-V10,...
Thyroid cancer is the most frequently occurring endocrine malignancy; however, preoperative diagnosis of some lesions, in particular those with follicular histology, difficult, and a consistent number not otherwise-specified "follicular nodules" are surgically resected more for than therapeutic purposes. In this study, we investigated whether lectin-related molecules CD44v6 galectin-3, expression which altered during deregulated cell growth malignant transformation, could be potential...
Combination therapy with BRAF and MEK inhibitors significantly improves survival in mutated melanoma patients but is unable to prevent disease recurrence due the emergence of drug resistance. Cancer stem cells (CSCs) have been involved these long-term treatment failures. We previously reported lung cancer that CSCs maintenance altered lipid metabolism dependent upon Stearoyl-CoA-desaturase (SCD1)-mediated upregulation YAP TAZ. On this ground, we investigated role SCD1 CSCs. gene expression...
Abstract The efficacy of breath volatile organic compounds (VOCs) analysis for the screening patients bearing breast cancer lesions has been demonstrated by using gas chromatography and artificial olfactory systems. On other hand, in-vitro studies suggest that VOCs detection could also give important indications regarding molecular tumorigenic characteristics tumor cells. Aim this study was to analyze in headspace cell lines order ascertain potentiality signatures giving information about...
The cell-surface heterodimers of the integrin family molecules, which mediate cell-cell and cell-substratum interactions, are likely to be functionally relevant in local metastatic tumor growth. In present study we have analyzed whether alpha 3/beta 1 receptor for collagen, laminin fibronectin undergoes changes expression during progression cutaneous malignant melanoma (CMM). results this demonstrated that, while low levels VLA3 detectable benign lesions, primary melanomas heterodimer...
Natural killer (NK) cells are major contributors to early defense against infections. Their effector functions controlled by a balance between activating and inhibiting signals. To date, however, the involvement of NK cell receptors signaling pathways in pathogens has not been extensively investigated. In mice, several coexpressed with function through immunoreceptor tyrosine-based activation motif (ITAM)-bearing molecule KARAP/DAP12. Here, we have analyzed role KARAP/DAP12 antiviral...
Galectin 3 (Gal-3), a member of the beta-galactoside binding lectin family, exhibits antiapoptotic functions, and its aberrant expression is involved in various aspects tumor progression. Here we show that p53-induced apoptosis associated with transcriptional repression Gal-3. Previously, it has been reported phosphorylation p53 at Ser46 important for transcription proapoptotic genes induction homeodomain-interacting protein kinase 2 (HIPK2) specifically these functions. We HIPK2 cooperates...
Checkpoint inhibitor-based immunotherapy is opening a promising scenario in oncology, with objective responses registered multiple cancer types. However, reliable predictive markers of tumor responsiveness are still lacking. These need to be urgently identified for better selection patients that can candidates immunotherapy. In this pilot study, cohort 34 consecutive bearing programmed death-ligand 1 (PD-L1)-positive non-small cell lung carcinoma (NSCLC), treated pembrolizumab, was...
Abstract Background Recent developments in abscopal effect strongly support the use of radiotherapy for treatment metastatic disease. However, deeper understanding molecular mechanisms underlying are required to best benefit a larger proportion patients with metastasis. Several groups including ours, reported involvement wild-type (wt) p53 radiation-induced effects, however very little is known on role wtp53 dependent mechanisms. Methods We investigated through vivo and vitro approaches how...
Abstract Background Metabolic syndrome represents a pancreatic ductal adenocarcinoma (PDAC) risk factor. alterations favor PDAC onset, which occurs early upon dysmetabolism. Pancreatic neoplastic lesions evolve within dense desmoplastic stroma, consisting in abundant extracellular matrix settled by cancer associated fibroblasts (CAFs). Hereby, dysmetabolism and association was analyzed focusing on CAF functions. Methods development dysmetabolic conditions investigated in: 1) high fat diet...
ABSTRACT CD44 is a polymorphic cell surface glycoprotein, currently proposed to be the principal receptor for hyaluronan. However, different isoforms of CD44, expressed in human lymphoid tumor cells, appear have distinct effects on ability cells attach hyaluronan-coated surfaces and their capacity form tumors vivo. In present study, we address mechanisms that may regulate isoform-dependent adhesion We use Burkitt lymphoma, stably transfected with six alternatively spliced isoforms, determine...