A5033510296- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Photonic and Optical Devices
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Advancements in Semiconductor Devices and Circuit Design
- DNA Repair Mechanisms
- Advanced Breast Cancer Therapies
- Lysosomal Storage Disorders Research
- Neuroblastoma Research and Treatments
- Amino Acid Enzymes and Metabolism
- Cellular transport and secretion
- Multiple Myeloma Research and Treatments
- Folate and B Vitamins Research
- Fish Ecology and Management Studies
- Advanced Fluorescence Microscopy Techniques
- Cancer-related gene regulation
- Advances in Oncology and Radiotherapy
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
New York University
2017-2025
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2021-2024
Indiana University School of Medicine
2018
Manhattanville College
2014
We present a modular approach to control the small molecule–mediated degradation of cellular proteins interest using light.
In response to nutrient deprivation, the cell mobilizes an extensive amount of membrane form and grow autophagosome, allowing progression autophagy. By providing membranes stimulating LC3 lipidation, COPII (Coat Protein Complex II) promotes autophagosome biogenesis. Here, we show that F-box protein FBXW5 targets SEC23B, a component COPII, for proteasomal degradation this event limits autophagic flux in presence nutrients. starvation, ULK1 phosphorylates SEC23B on Serine 186, preventing...
Although mismatch repair (MMR) is essential for correcting DNA replication errors, it can also recognize other lesions, such as oxidized bases. In G0 and G1, MMR kept in check through unknown mechanisms error-prone during these cell cycle phases. We show that mammalian cells, D-type cyclins are recruited to sites of oxidative damage a PCNA- p21-dependent manner. inhibit the proteasomal degradation p21, which competes with proteins binding PCNA, thereby inhibiting MMR. The ability limit CDK4-...
FBXO31 is the substrate receptor of one many CUL1-RING ubiquitin ligase (CRL1) complexes. Here, we show that low mRNA levels are associated with high pre-operative prostate-specific antigen (PSA) and Gleason grade in human prostate cancer. Mechanistically, CRL1
In healthy cells, cyclin D1 is expressed during the G1 phase of cell cycle, where it activates CDK4 and CDK6. Its dysregulation a well-established oncogenic driver in numerous human cancers. The cancer-related function has been primarily studied by focusing on phosphorylation retinoblastoma (RB) gene product. Here, using an integrative approach combining bioinformatic analyses biochemical experiments, we show that GTSE1 (G-Two S phases protein 1), positively regulating cycle progression,...
In healthy cells, cyclin D1 is expressed during the G1 phase of cell cycle, where it activates CDK4 and CDK6. Its dysregulation a well-established oncogenic driver in numerous human cancers. The cancer-related function has been primarily studied by focusing on phosphorylation retinoblastoma (RB) gene product. Here, using an integrative approach combining bioinformatic analyses biochemical experiments, we show that GTSE1 (G-Two S phases protein 1), positively regulating cycle progression,...
Malathion, a common organophosphate insecticide, is proven acetylcholinesterase inhibitor and the most applied insecticide in United States. The use of zebrafish as model to study effects pesticides on development an innovative approach yielding relevant implications for determining potential toxic these humans. In this study, simple noninvasive technique was developed investigate cardiotoxicity malathion Danio rerio embryos, detect quantify its effect heart rate. Videos were recorded under...
ABSTRACT The large majority of oxidative DNA lesions occurring in the G1 phase cell cycle are repaired by base excision repair (BER) rather than mismatch (MMR) to avoid long resections that can lead genomic instability and death. However, molecular mechanisms dictating pathway choice between MMR BER have remained unknown. Here, we show that, during G1, D-type cyclins recruited sites damage a PCNA- p21-dependent manner. shield p21 from its two ubiquitin ligases CRL1 SKP2 CRL4 CDT2...
PROTACs (proteolysis targeting chimeras) are bifunctional molecules that tag proteins for ubiquitylation by an E3 ligase complex and subsequent degradation the proteasome. They have emerged as powerful tools to control levels of specific cellular on verge being clinically used. We now introduce photoswitchable can be activated with temporal spatial precision light provides. These trifunctional molecules, which we named PHOTACs, consist a ligand ligase, photoswitch, protein interest....
PROTACs (proteolysis targeting chimeras) are bifunctional molecules that tag proteins for ubiquitylation by an E3 ligase complex and subsequent degradation the proteasome. They have emerged as powerful tools to control levels of specific cellular on verge being clinically used. We now introduce photoswitchable can be activated with temporal spatial precision light provides. These trifunctional molecules, which we named PHOTACs, consist a ligand ligase, photoswitch, protein interest....
<p><i>PROTACs (proteolysis targeting chimeras) are bifunctional molecules that tag proteins for ubiquitylation by an E3 ligase complex and subsequent degradation the proteasome. They have emerged as powerful tools to control levels of specific cellular on verge being clinically used. We now introduce photoswitchable PROTACs can be activated with temporal spatial precision light provides. These trifunctional molecules, which we named PHOTACs, consist a ligand ligase, photoswitch,...
D-type cyclins (cyclin D1, D2, and D3, together cyclin D) are central drivers of the cell division cycle well-described proto-oncoproteins. Rapid turnover D is critical for its regulation, but underlying mechanism has remained a matter debate. Recently, AMBRA1 was identified as major regulator stability all three cyclins. serves substrate receptor one ∼40 CUL4-RING E3 ubiquitin ligase (CRL4) complexes to mediate polyubiquitylation subsequent degradation D. Consequently, regulates...
Cyclin D1 is the activating subunit of cell cycle kinases CDK4 and CDK6, its dysregulation a well-known oncogenic driver in many human cancers. The biological function cyclin has been primarily studied by focusing on phosphorylation retinoblastoma (RB) gene product. Here, using an integrative approach combining bioinformatic analyses biochemical experiments, we show that GTSE1 (G2 S phases expressed protein 1), positively regulating progression, previously unknown substrate D1-CDK4/6....
Cyclin D1 is the activating subunit of cell cycle kinases CDK4 and CDK6, its dysregulation a well-known oncogenic driver in many human cancers. The biological function cyclin has been primarily studied by focusing on phosphorylation retinoblastoma (RB) gene product. Here, using an integrative approach combining bioinformatic analyses biochemical experiments, we show that GTSE1 (G2 S phases expressed protein 1), positively regulating progression, previously unknown substrate D1-CDK4/6....
Cyclin D1 is the activating subunit of cell cycle kinases CDK4 and CDK6, its dysregulation a well-known oncogenic driver in many human cancers. The biological function cyclin has been primarily studied by focusing on phosphorylation retinoblastoma (RB) gene product. Here, using an integrative approach combining bioinformatic analyses biochemical experiments, we show that GTSE1 (G2 S phases expressed protein 1), positively regulating progression, previously unknown substrate D1-CDK4/6....
ABSTRACT In response to nutrient deprivation, the cell needs mobilize an extensive amount of membrane form and grow autophagosome, allowing progression autophagy. By providing membranes a source for LC3 lipidation, COPII (Coat Protein Complex II) localizes endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) promotes autophagosome biogenesis. However, molecular mechanisms that, in starvation, divert from secretory pathway autophagic are largely unknown. Here, we show that F-box...