A5075521862- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Pharmacological Receptor Mechanisms and Effects
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
- Protein Degradation and Inhibitors
- Cancer, Lipids, and Metabolism
- Cancer-related molecular mechanisms research
- Effects of Radiation Exposure
- Extracellular vesicles in disease
- Cancer therapeutics and mechanisms
- MicroRNA in disease regulation
- Glioma Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Virus-based gene therapy research
- Caveolin-1 and cellular processes
- Molecular Biology Techniques and Applications
- HER2/EGFR in Cancer Research
- Mathematical Biology Tumor Growth
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
Glioblastoma (GBM) is the most lethal brain tumor in adults. Radiation, together with temozolomide standard treatment, but nevertheless, relapse occurs nearly all cases. Understanding mechanisms underlying radiation resistance may help to find more effective therapies. After ATP released into microenvironment where it binds and activates purinergic P2 receptors, mainly of P2X7 subtype. Two main splice variants, P2X7A P2X7B, are expressed cell types, they associate distinct biochemical...
// Chiara Arienti 1 , Michele Zanoni Sara Pignatta Alberto Del Rio 2, 3 Silvia Carloni Michela Tebaldi Gianluca Tedaldi Anna Tesei Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy 2 Institute of Organic Synthesis and Photoreactivity (ISOF), National Research Council (CNR), Bologna, Innovamol Srls, Modena, Correspondence to: Arienti, e-mail: chiara.arienti@irst.emr.it Tesei, anna.tesei@irst.emr.it Keywords: trastuzumab...
Abstract Background Recent developments in abscopal effect strongly support the use of radiotherapy for treatment metastatic disease. However, deeper understanding molecular mechanisms underlying are required to best benefit a larger proportion patients with metastasis. Several groups including ours, reported involvement wild-type (wt) p53 radiation-induced effects, however very little is known on role wtp53 dependent mechanisms. Methods We investigated through vivo and vitro approaches how...
Abstract Purpose: Despite tumor resection being the first-line clinical care for glioblastoma (GBM) patients, nearly all preclinical immune therapy models intend to treat established GBM. Characterizing cytoreductive surgery-induced response combined with administration of cytokines has potential offering a new treatment paradigm GBMs. Experimental Design: We developed syngeneic orthotopic mouse GBM and characterized intact resected tumors. also created highly secretable variant cytokine...
Although two-dimensional (2-D) monolayer cell cultures provide important information on basic tumor biology and radiobiology, they are not representative of the complexity three-dimensional (3-D) solid tumors. In particular, new models reproducing clinical conditions as closely possible needed for radiobiological studies to that can be translated from bench bedside. We developed a novel system irradiation, under sterile conditions, 3-D spheroids, in vitro model considered bridge between...
// Sandra Durante 1,* , Isabella Orienti 2,* Gabriella Teti 1 Viviana Salvatore Stefano Focaroli Anna Tesei 3 Sara Pignatta and Mirella Falconi DIBINEM—Department of Biomedical Neuromotor Sciences, University Bologna, Via Irnerio 48, 40126, Italy 2 FaBiT–Department Pharmacy Biotechnology, San Donato 19, 240127, Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori(IRST) IRCCS, via P. Maroncelli 40, 47014, Meldola, FC, * These authors contributed equally to...
Human glioblastoma (GBM) is one of the most feared primary malignant brain tumors. We investigated effect hyperbaric oxygen (HBO) on GBM patient-derived cells and microglia cell biology (CHME-5). HBO administered to inhibited proliferation, downregulated hypoxia-inducible factor 1 α (HIF-1α) expression, induced glucose metabolism reprogramming (glucose rewiring). It also affected ability a perpetuate its lineage, give rise differentiated interact with environment maintain balance between...
E-cadherin is a key player in gastric cancer (GC) and germline alterations of CDH1, its encoding gene, are responsible for Hereditary Diffuse Gastric Cancer (HDGC) syndrome. This study aimed at elucidating the role genetic variants DNA methylation CDH1 promoter enhancers regulation gene expression. For this purpose, we analyzed through Next-Generation Sequencing (NGS) series GC cell lines (NCI-N87, KATO-III, SNU-1, SNU-5, GK2, AKG, KKP) corresponding expression levels. By bisulfite genomic...
Introduction: Pancreatic cancer (PC) is one of the most lethal tumor worldwide, with no prognosis improvement over past 20-years. The silent progressive nature this neoplasia hampers early diagnosis, and surgical resection tumor, thus chemotherapy remains only available therapeutic option. Sigma receptors (SRs) are a class proposed as new targets due to their over-expression in cells involvement biology. main localization these strongly suggests potential role ER unfolded protein response...
HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence reported ductal lavage fluids blood specimens.We analyzed 50 ND, 22 vesicles as well 51 pathologic breast tissues for the of 16 types.We show that ND is predictive DNA-positive lesions more represented intraductal papillomas. We also vesicles.Our data supports use liquid biopsy to detect pathology.
The management of hormone-refractory prostate cancer represents a major challenge in the therapy this tumor, and identification novel androgen receptor antagonists is needed to render treatment more effective. We analyzed activity two antagonists, (S)-11 (R)-9, vitro vivo experimental models hormone-sensitive or castration-resistant (CRPC). In experiments were performed on LNCaP, LNCaP-AR, LNCaP-Rbic VCaP human cells. Cytotoxic was assessed by SRB BrdU uptake, AR transactivation luciferase...
Background aimsDendritic cells (DCs) are professional antigen-presenting of the mammalian immune system. Ex vivo differentiated DCs represent a unique Advanced Therapy Medicinal Product (ATMP), used in several clinical trials as personalized cancer immunotherapy. The therapy's reliability depends on its capacity to produce high-quality mature (mDCs) compliance with Good Manufacturing Practices.AimsFrom March 2010 December 2023, 103 patients were enrolled multiple at Immuno-Gene Factory IRCCS...
Since its discovery in the late 19th century, radiotherapy has been one of most important medical treatments oncology. Recently, fasting or short-term starvation (STS) cancer patients undergoing chemotherapy studied to determine potential for enhancing therapeutic index and preventing side- effects, but no data are available setting. We thus decided investigate effects vitro STS combination with metastatic cells non-cancer cells. Cells were incubated medium (STS medium, 0·5 g/L glucose + 1%...
Advanced therapy medical products (ATMPs) are rapidly growing as innovative medicines for the treatment of several diseases. Hence, role quality analytical tests to ensure consistent product safety and has become highly relevant. Several clinical trials involving dendritic cell (DC)-based vaccines cancer ongoing at our institute. The DC-based vaccine is prepared via CD14+ monocyte differentiation. A fresh dose 10 million DCs administered patient, while remaining aliquoted, frozen, stored in...
Comet assay provides an easy solution to estimate DNA damage in single cells through microscopy assessment. It is widely used the analysis of genotoxic damages induced by radiotherapy or chemotherapeutic agents. quantified at single-cell level computing displacement between genetic material within nucleus, typically called "comet head", and surrounding part cell, considered as tail". Today, number works based on Assay analyses really impressive. In this work, besides revising solutions...
Although combination chemotherapy and radiotherapy have become the standard of care in numerous tumors, mechanisms interaction are often still unclear. The purpose this study was to analyze efficacy radiation treatment cisplatin sequences investigate their interaction. Three melanoma cell lines were used evaluate vitro radiation-induced cytotoxicity before after treatment. Expression levels a panel genes determined by real-time RT-PCR. Cytotoxic effect evaluated flow cytometry analysis Comet...
Pancreatic cancer (PC) remains one of the most lethal cancers worldwide. Sigma receptors (SRs) have been proposed as therapeutic targets. Their main localization suggests they play a potential role in ER stress and triggering unfolded protein response (UPR). Here, we investigated mechanisms action RC-106, novel pan-SR modulator, to characterize therapeutically exploitable SRs tumors. Two PC cell lines were used all experiments. Terminal UPR activation was evaluated by quantifying BiP, ATF4...