A5088505960- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- BRCA gene mutations in cancer
- Colorectal Cancer Screening and Detection
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Bladder and Urothelial Cancer Treatments
- Chronic Lymphocytic Leukemia Research
- DNA Repair Mechanisms
- Advanced Breast Cancer Therapies
- Prostate Cancer Treatment and Research
- Lung Cancer Research Studies
- Telomeres, Telomerase, and Senescence
- Cancer-related gene regulation
- Male Breast Health Studies
- Cancer-related molecular mechanisms research
- Chronic Myeloid Leukemia Treatments
- Lung Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Nutrition, Genetics, and Disease
- Molecular Biology Techniques and Applications
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
University of Manchester
2022
Ansaldo (Italy)
2018
Bioscience (China)
2016
Ospedale G.B. Morgagni - L.Pierantoni
2000-2009
Istituto Oncologico Romagnolo
2000-2007
Medica (Italy)
2004
Sidac (Italy)
2004
University of Pavia
1996-2000
Abstract Purpose: To analyze the impact of TP53 mutations on response to first-line tyrosine kinase inhibitors (TKI) in patients with EGFR-mutated non–small cell lung cancer (NSCLC). Experimental Design: 136 NSCLC receiving TKIs were analyzed. evaluated 123 relation disease control rate (DCR), objective (ORR), progression-free survival (PFS), and overall (OS). Results: observed 37 (30.1%), 10 (27.0%), 6 (16.2%), 9 (24.3%), 12 (32.4%) exons 5, 6, 7, 8, respectively. DCR was 70% TP53-mutated...
This study aimed to investigate copy number variations (CNVs) of CYP17A1 and androgen receptor (AR) genes in serum cell-free DNA collected before starting abiraterone 53 consecutive patients with castration-resistant prostate cancer (CRPC). Serum was isolated CNVs were analysed for AR using Taqman assays. The association between progression-free/overall survival (PFS/OS) evaluated by the Kaplan–Meier method log-rank test. Median PFS gene gain 2.8 vs 9.5 months non-gained cases (P<0.0001)....
Malignant pleural mesothelioma (MPM) is a progressive malignancy associated to the exposure of asbestos fibers. The most frequently inactivated tumor suppressor gene in MPM CDKN2A/ARF, encoding for cell cycle inhibitors p16INK4a and p14ARF, deleted about 70% cases. Considering high frequency alterations this gene, we tested cells efficacy palbociclib (PD-0332991), highly selective inhibitor cyclin-dependent kinase (CDK) 4/6. analyses were performed on panel lines two primary culture from...
Gut microbiota has been implicated in the etiopathogenesis of colorectal cancer. The development cancer is a multistep process by which healthy epithelium slowly develops into preneoplastic lesions, turn progress malignant carcinomas over time. In particular, sporadic cancers can arise from adenomas (about 85% cases) or serrated polyps through "adenoma-carcinoma" "serrated polyp-carcinoma" sequences, respectively. this study, we performed 16 S rRNA gene sequencing bacterial DNA extracted...
Investigation of extensively sampled nontumor gastric mucosa from 205 early cancers showed Helicobacter pylori colonization in 85% cases, including 100% diffuse and 78% (83% 97 cases with Swiss rolls) glandular or mixed cancers. Intestinal metaplasia, its type III variant, was prominent the associated (but not diffuse) Both (usually called "intestinal") diffuse-type admixtures intestinal tumor cell phenotypes. p53 gene mutations protein immunostaining were essentially restricted to...
The identification of new molecular markers for the early detection colorectal cancer has become an important objective. We compared sensitivity and specificity free circulating DNA with that more conventional carcinoembryonic antigen (CEA) evaluated two in combination.The study was carried out on 75 healthy donors patients. Free determined serum quantitative PCR analysis. diagnostic accuracy each assay calculated using receiver operating characteristic (ROC) curves. relevance two-marker...
GSTP1 belongs to the GSTs family, a group of enzymes involved in detoxification exogenous substances and it also plays an important role cell cycle regulation. Its dysregulation correlates with large variety tumors, particular prostate cancer. We investigated methylation status specific PCR (MS-PCR) cancer (PCa) benign tissue 56 prostatectomies. performed immunohistochemistry (IHC) so as correlate gene silencing. appears methylated PCa not healthy tissue; IHC confirmed that leads protein...
As new genes predisposing to breast (BC) and ovarian cancer (OC) are constantly emerging, the use of panels analyzed by Next-Generation Sequencing (NGS) is increasing in clinical diagnostics. The identification a large number germline mutations allows for deeper knowledge predisposition, although raising many questions about patient management.BC OC patients recruited our counseling service between 2012-2015 were included this study. DNA was extracted from peripheral blood panel 94 involved...
// Samanta Salvi 1 , Valentina Casadio Vincenza Conteduca 2 Cristian Lolli Giorgia Gurioli Filippo Martignano 1, 4 Giuseppe Schepisi Sara Testoni 3 Emanuela Scarpi Dino Amadori Daniele Calistri Gerhardt Attard 5 Ugo De Giorgi Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy Department of Medical Oncology, Unit Biostatistics and Clinical Trials, University Florence, The Institute Cancer Research Royal Marsden, London, UK...
Targeting angiogenesis in the treatment of colorectal cancer (CRC) is a common strategy, for which potential predictive biomarkers have been studied. miRNAs are small non-coding RNAs involved several processes including angiogenic pathway. They very stable biological fluids, turns them into circulating biomarkers. In this study, we considered case series patients with metastatic (m) CRC treated bevacizumab (B)-based treatment, enrolled prospective multicentric Italian Trial Advanced...
Circulating cell-free DNA has been recognized as an accurate marker for the diagnosis of prostate cancer, whereas role urine (UCF DNA) never evaluated in this setting. It is known that normal apoptotic cells produce highly fragmented while cancer release longer DNA. We thus verified potential UCF integrity early diagnosis. was isolated from 29 patients and 25 healthy volunteers. Sequences than 250 bp (c-Myc, BCAS1, HER2) were quantified by real-time PCR to verify integrity. Receiver...
Breast cancer (BC) in men is rare and genetic predisposition likely to play a relevant role its etiology. Inherited mutations BRCA1/2 account for about 13% of all cases additional genes that may contribute the missing heritability need be investigated. In our study, well‐characterized series 523 male BC (MBC) patients from Italian multicenter study on MBC, enriched non‐ MBC cases, was screened by multigene custom panel 50 cancer‐associated genes. The main clinical‐pathologic characteristics...
Abstract Gene mutations in APC , K‐ras and p53 are thought to be essential events for colorectal cancer development. Recent data seem indicate that rarely co‐exist the same tumor, indicating these alterations do not represent a synergistic evolutionary pathway. Moreover, an inverse relation between gene activation BRAF has been demonstrated, suggesting alternative pathways transformation. To reconstruct chronological modulation of during cell transformation progression, genes were analyzed...
Introduction . The detection of tumor-specific markers in urine has paved the way for new early noninvasive diagnostic approaches prostate cancer. We evaluated DNA integrity supernatant to verify its capacity discriminate between cancer and benign diseases urogenital tract. Patients Methods A total 131 individuals were enrolled: 67 patients 64 with tract (control group). Prostate-specific antigen (PSA) levels determined. Urine cell-free (UCF) was isolated sequences longer than 250 bp...
There is evidence of a different response to treatment with regard the primary tumor localization (right-sided or left-sided) in patients metastatic colorectal cancer (mCRC). We analyzed outcomes and biomolecular characteristics relation 122 370 enrolled onto phase III prospective multicenter “Italian Trial Advanced Colorectal Cancer (ITACa)”, randomized receive first-line chemotherapy (CT) CT plus bevacizumab (CT + B). RAS BRAF mutations; baseline expression levels circulating vascular...