A5016213425- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Bladder and Urothelial Cancer Treatments
- Radiopharmaceutical Chemistry and Applications
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Urological Disorders and Treatments
- Prostate Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Renal and related cancers
- Renal cell carcinoma treatment
- Hormonal and reproductive studies
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Urinary and Genital Oncology Studies
- Cancer Research and Treatments
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Colorectal Cancer Screening and Detection
- Medical Imaging Techniques and Applications
- Cancer-related gene regulation
- Colorectal Cancer Surgical Treatments
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2010-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2021
Bioscience (China)
2016
University of Bologna
1995
Androgen receptor mutations and amplifications in circulating tumor DNA provide clues to prostate cancer drug resistance.
BackgroundThere is an urgent need to identify biomarkers guide personalized therapy in castration-resistant prostate cancer (CRPC). We aimed clinically qualify androgen receptor (AR) gene status measurement plasma DNA using multiplex droplet digital PCR (ddPCR) pre- and post-chemotherapy CRPC.MethodsWe optimized ddPCR assays for AR copy number mutations retrospectively analyzed from patients recruited one of the three biomarker protocols with prospectively collected clinical data. evaluated...
This study aimed to investigate copy number variations (CNVs) of CYP17A1 and androgen receptor (AR) genes in serum cell-free DNA collected before starting abiraterone 53 consecutive patients with castration-resistant prostate cancer (CRPC). Serum was isolated CNVs were analysed for AR using Taqman assays. The association between progression-free/overall survival (PFS/OS) evaluated by the Kaplan–Meier method log-rank test. Median PFS gene gain 2.8 vs 9.5 months non-gained cases (P<0.0001)....
GSTP1 belongs to the GSTs family, a group of enzymes involved in detoxification exogenous substances and it also plays an important role cell cycle regulation. Its dysregulation correlates with large variety tumors, particular prostate cancer. We investigated methylation status specific PCR (MS-PCR) cancer (PCa) benign tissue 56 prostatectomies. performed immunohistochemistry (IHC) so as correlate gene silencing. appears methylated PCa not healthy tissue; IHC confirmed that leads protein...
// Samanta Salvi 1 , Valentina Casadio Vincenza Conteduca 2 Cristian Lolli Giorgia Gurioli Filippo Martignano 1, 4 Giuseppe Schepisi Sara Testoni 3 Emanuela Scarpi Dino Amadori Daniele Calistri Gerhardt Attard 5 Ugo De Giorgi Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy Department of Medical Oncology, Unit Biostatistics and Clinical Trials, University Florence, The Institute Cancer Research Royal Marsden, London, UK...
Circulating cell-free DNA has been recognized as an accurate marker for the diagnosis of prostate cancer, whereas role urine (UCF DNA) never evaluated in this setting. It is known that normal apoptotic cells produce highly fragmented while cancer release longer DNA. We thus verified potential UCF integrity early diagnosis. was isolated from 29 patients and 25 healthy volunteers. Sequences than 250 bp (c-Myc, BCAS1, HER2) were quantified by real-time PCR to verify integrity. Receiver...
Introduction . The detection of tumor-specific markers in urine has paved the way for new early noninvasive diagnostic approaches prostate cancer. We evaluated DNA integrity supernatant to verify its capacity discriminate between cancer and benign diseases urogenital tract. Patients Methods A total 131 individuals were enrolled: 67 patients 64 with tract (control group). Prostate-specific antigen (PSA) levels determined. Urine cell-free (UCF) was isolated sequences longer than 250 bp...
Although non muscle invasive bladder cancer (NMIBC) generally has a good long-term prognosis, up to 80% of patients will nevertheless experience local recurrence after the primary tumor resection. The search for markers capable accurately identifying at high risk is ongoing. We retrospectively evaluated methylation status panel 24 suppressor genes (TIMP3, APC, CDKN2A, MLH1, ATM, RARB, CDKN2B, HIC1, CHFR, BRCA1, CASP8, CDKN1B, PTEN, BRCA2, CD44, RASSF1, DAPK1, FHIT, VHL, ESR1, TP73, IGSF4,...
Although numerous studies have focused on the link between CpG island methylator phenotypes and development of colorectal cancer, few dealt specifically with methylation profiling in rectal cancer its role predicting response to neoadjuvant chemoradiotherapy (NCRT). We characterized profiles normal neoplastic tissue samples from patients assessed this molecular profile chemoradioactivity. evaluated 74 pretreatment tumor 16 apparently biopsies submitted NCRT. The 24 different suppressor genes...
// Roberta Maltoni 1 , Valentina Casadio 2, * Sara Ravaioli Flavia Foca 3 Maria Maddalena Tumedei 2 Samanta Salvi Filippo Martignano Daniele Calistri Andrea Rocca Alessio Schirone Dino Amadori Bravaccini Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy Biosciences Laboratory, Unit Biostatistics and Clinical Trials, These authors contributed equally to this work Correspondence to: Bravaccini, email:...
Recently, mixed forms between adenocarcinoma and neuroendocrine prostate cancer (NEPC) have emerged that are characterized by persistent androgen receptor (AR)-signalling elevated chromogranin A (CgA) levels. The main aim of this study was to analyze castration-resistant (CRPC) patients treated with abiraterone or enzalutamide, assessing progression-free/overall survival (PFS/OS) in association circulating AR CgA. aberrations were analyzed droplet digital PCR pre-treatment plasma samples...
Epigenetic silencing mediated by CpG island methylation is a common feature of many cancers. Characterizing aberrant DNA changes associated with prostate carcinogenesis could potentially identify tumour-specific pattern, facilitating the early diagnosis cancer. The objective study was to assess status 40 tumour suppressor genes in cancer and healthy prostatic tissues.We used specific-multiplex ligation probe amplification (MS-MLPA) assay two independent case series (training validation set)....
The aim of the present study was to identify sensitive and noninvasive biomarkers early carcinogenic effect at target organ use in biomonitoring studies workers risk for previous occupational exposure potential carcinogens. Standard urine cytology (Papanicolaou staining test), comet assay, quantitative telomerase repeat amplification protocol (TRAP) assay were performed 159 ex-rubber employed tyres production 97 unexposed subjects. In TRAP positive cases, a second level analysis using FISH...
// Giuseppe Schepisi 1 , Silvia De Padova Emanuela Scarpi Cristian Lolli Giorgia Gurioli Cecilia Menna Salvatore L. Burgio Lorena Rossi Valentina Gallà Casadio Samanta Salvi Vincenza Conteduca and Ugo Giorgi Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Via P. Maroncelli, 40 I-47014 Meldola (FC), Italy Correspondence to: Schepisi, email: giuseppe.schepisi@irst.emr.it Keywords: germ cell tumor, testicular cancer survivors, 25-hydroxyvitamin D, testosterone,...
Prostate Cancer (PCa) is one of the most frequently identified urological cancers. PCa patients are often over-diagnosed due to still not highly specific diagnostic methods. The need for more accurate tools prevent overestimated diagnosis and unnecessary treatment with non-malignant conditions clear, new markers methods strongly desirable. Extracellular vesicles (EVs) hold great promises as liquid biopsy-based markers. Despite biological technical issues present in their detection study,...
Although the presence of circulating cell-free DNA in plasma or serum has been widely shown to be a suitable source biomarkers for many types cancer, few studies have focused on potential use urine (UCF) DNA. Starting from hypotheses that normal apoptotic cells produce highly fragmented and cancer release longer DNA, role UCF integrity was evaluated as an early diagnostic marker capable distinguishing between patients with prostate bladder healthy individuals. A analysis is proposed basis...
Patients with non-muscle invasive bladder cancer (NMIBC) generally have a high risk of relapsing locally after primary tumor resection. The search for new predictive markers local recurrence thus represents an important goal the management this disease. We studied copy number variations (CNVs) 24 oncogenes (MDM4, MYCN, ALK, PDGFRA, KIT, KDR, DHFR, EGFR, MET, SMO, FGFR1, MYC, ABL1, RET, CCND1, CCND2, CDK4, MDM2, AURKB, ERBB2, TOP2A, AURKA, AR and BRAF) using multiplex ligation probe...