A5059596228- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Muscle Physiology and Disorders
- Retinal Diseases and Treatments
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Sulfur Compounds in Biology
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- TGF-β signaling in diseases
- Retinal Imaging and Analysis
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Glaucoma and retinal disorders
- Complement system in diseases
- Redox biology and oxidative stress
- Caveolin-1 and cellular processes
- Macrophage Migration Inhibitory Factor
- Lymphatic System and Diseases
- Ocular Diseases and Behçet’s Syndrome
- Nerve injury and regeneration
- Retinal and Macular Surgery
- Clusterin in disease pathology
- Cardiomyopathy and Myosin Studies
University of Siena
2016-2025
Accademia Albertina delle Belle Arti
2015
Siena Biotech (Italy)
2010
Novartis (Italy)
2010
University of Padua
1994-1995
Guy's Hospital
1995
University of Oxford
1994
Triple-negative breast cancer (TNBC) is a very aggressive and heterogeneous group of tumors. In order to develop effective therapeutic strategies, it therefore essential identify the subtype-specific molecular mechanisms underlying disease progression resistance chemotherapy. TNBC cells are highly dependent on exogenous cystine, provided by overexpression cystine/glutamate antiporter SLC7A11/xCT, fuel glutathione synthesis promote an oxidative stress response consistent with their high...
We identified and compared secreted microRNA (miRNA) expression in aqueous humor (AH) plasma samples among patients with: type 2 diabetes mellitus (T2D) complicated by non-proliferative diabetic retinopathy (DR) associated with macular edema (DME) (DME group: 12 patients); T2D without DR (D 8 non-diabetic (CTR 10 patients). Individual patient AH from five subjects each group were profiled on TaqMan Low Density MicroRNA Array Cards. Differentially expressed miRNAs profiling then validated...
Integrins cooperate with growth factor receptors to promote downstream signaling for cell proliferation and migration. However, the mechanism of receptor activation is still unknown.To analyze phosphorylation vascular endothelial (VEGFR)-3 by adhesion.We show that VEGFR-3 phosphorylation, induced attachment extracellular matrix, independent from intrinsic kinase activity receptor, as evidenced adhesion experiments a mutant dead or in presence specific inhibitor MAZ 51. Cell c-Src PP2...
Purpose: The purpose of this study was to evaluate the expression high-temperature requirement A serine peptidase 1 (HTRA1), TGF-β1, bone morphogenetic protein 4 (BMP4), growth differentiation factor 6 (GDF6), and VEGFA proteins in aqueous humor patients with naïve choroidal neovascularization (nCNV) secondary AMD. Methods: We measured by ELISA concentrations HTRA1, BMP4, GDF6, 23 affected nCNV who received three consecutive monthly intravitreal injections 0.5 mg ranibizumab. Samples were...
// Federico Galvagni 1, * , Federica Nardi Marco Maida 1 Giulia Bernardini Silvia Vannuccini 2 Felice Petraglia Annalisa Santucci Maurizio Orlandini Department of Biotechnology, Chemistry and Pharmacy, University Siena, 2-53100 Italy Molecular Developmental Medicine, Obstetrics Gynecology, 53100 These authors contributed equally to this work Correspondence to: Orlandini, e-mail: maurizio.orlandini@unisi.it Keywords: angiogenesis, signal transduction, C1qRp, Src, Cbl Received: June 29, 2015...
// Maurizio Orlandini 1 , Federico Galvagni Monia Bardelli 1,5 Marina Rocchigiani Claudia Lentucci Francesca Anselmi Alessio Zippo 1,6 Luca Bini 2 Salvatore Oliviero 3,4 Department of Biotechnology, Chemistry and Pharmacy, University Siena, Via A. Moro, – 53100 Italy. Life Sciences, 3 HuGeF, Nizza, 52 10126 Torino, 4 Sciences Systems Biology, Accademia Albertina, 13 10123 5 Novartis Vaccines, Fiorentina, 6 INGM, F. Sforza, 35 20122 Milano, Correspondence: Orlandini, email: Keywords :...
Ten-eleven translocation (Tet) genes encode for a family of hydroxymethylase enzymes involved in regulating DNA methylation dynamics. Tet1 is highly expressed mouse embryonic stem cells (ESCs) where it plays critical role the pluripotency maintenance. also cell reprogramming events and cancer progression. Although functional has been largely studied, its regulation poorly understood. Here we show that gene regulated, both human ESCs, by stemness specific factors Oct3/4, Nanog Myc. Thus...
To form three-dimensional capillary tubes, endothelial cells must establish contacts with the extracellular matrix that provides signals for their proliferation, migration, and differentiation. The transcription factor Fosl1 plays a key role in vasculogenic angiogenic processes as knockout embryos die vascular defects extraembryonic tissues. Here, we show Fosl1(-/-) embryonic stem differentiate into but fail to correctly assemble primitive capillaries tube-like structures. FOSL1 silencing...
In the endothelium, single-pass membrane protein CD93, through its interaction with extracellular matrix Multimerin-2, activates signaling pathways that are critical for vascular development and angiogenesis. Trafficking of adhesion molecules endosomal compartments modulates their output. However, mechanistic basis coordinating CD93 recycling implications endothelial cell (EC) function remain elusive.Human umbilical vein ECs (HUVECs) human dermal blood (HDBEC) were used in this study....
Oxidation processes in mitochondria and different environmental insults contribute to unwarranted accumulation of reactive oxygen species (ROS). These, turn, rapidly damage intracellular lipids, proteins, DNA, ultimately causing aging several human diseases. Cells have developed very effective systems control ROS levels. Among these, removal excessive amounts is guaranteed by upregulated expression various antioxidant enzymes, through activation the NF-E2-Related Factor 2 (NRF2) protein....
We have analysed the sequence organization of pseudoautosomal region at telomeres long arms human sex chromosomes and shown that It is 320 kb long. A LINE Is present on both X Y immediately adjacent to breakpoint in homology suggesting arose as a result an ectopic recombination event mediated by sequences originally non-homologous stretches chromosomal DNA. This led translocatlon from chromosome telomere onto created new region.
Growth factor stimulation induces c-Jun-dependent survival of primary endothelial cells. However, the mechanism c-Jun anti-apoptotic activity has not been identified. We here demonstrate that in response to growth treatment, human cells as well mouse fibroblasts respond with an increased expression forms a complex ATF2. This activates protein Bcl-X(L). By site-directed mutagenesis experiments, we identified two AP-1-binding sites located within proximal promoter Bcl-X gene. Site-directed...
ABSTRACT Sustained signalling at the immune synapse (IS) requires synaptic delivery of recycling endosome-associated T cell antigen receptors (TCRs). IFT20, a component intraflagellar transport system, controls TCR to IS as complex with IFT57 and IFT88. Here, we used quantitative mass spectrometry identify additional interaction partners IFT20 in Jurkat cells. In addition IFT88, analysis revealed new binding partners, including IFT54 (also known TRAF3IP1), GMAP-210 TRIP11), Arp2/3 subunit-3...