A5044756351- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Ubiquitin and proteasome pathways
- Cytokine Signaling Pathways and Interactions
- HER2/EGFR in Cancer Research
- Hormonal and reproductive studies
- Steroid Chemistry and Biochemistry
- Reproductive System and Pregnancy
- Receptor Mechanisms and Signaling
- Biochemical effects in animals
- Enzyme Production and Characterization
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- FOXO transcription factor regulation
- Retinoids in leukemia and cellular processes
- Cancer Cells and Metastasis
- Prostate Cancer Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Nutrition, Genetics, and Disease
- Cell Adhesion Molecules Research
- Amino Acid Enzymes and Metabolism
- Protein Tyrosine Phosphatases
- RNA Research and Splicing
- Chemical Synthesis and Analysis
University of Campania "Luigi Vanvitelli"
2016-2025
University of Naples Federico II
2014-2023
The University of Tokyo
2022
University of Strathclyde
2022
Imperial College London
2022
University of California, Berkeley
2022
University of Pavia
2022
IIT@MIT
2022
Northwestern University
2022
University of California, San Diego
2022
AbstractIn breast cancer cells, estrogens activate the Src/Erk pathway through an interaction of estrogen receptor alpha (ERα) with SH2 domain c-Src. Progestins have been reported to also this either via progesterone isoform B (PRB) ERα, which itself activates c-Src, or by direct PRB SH3 Here we identify two domains PRB, ERID-I and -II, mediating a ligand-binding ERα. ERID-II flank proline cluster responsible for binding In mammalian ERα progestin activation cascade are abolished deletion...
Abstract Under conditions of short-term hormone deprivation, epidermal growth factor (EGF) induces DNA synthesis, cytoskeletal changes, and Src activation in MCF-7 LNCaP cells. These effects are drastically inhibited by pure estradiol or androgen antagonists, implicating a role the steroid receptors these findings. Interestingly, EGF triggers rapid association with receptor (AR) α (ERα) cells ERβ Here, we show that, through (EGFR) erb-B2, tyrosine phosphorylation ER preassociated AR, thereby...
Abstract A procedure is presented for the purification of l-tyrosine-2-oxoglutarate aminotransferase from livers rats previously treated with synthetic glucocorticosteroid, triamcinolone (9α-fluoro-16α-hydroxyprednisolone diacetate). 2500-fold over crude liver extract was obtained. The final preparation, which appeared to be 95% homogeneous by ultracentrifugal and electrophoretic criteria, has twice specific activity most active fraction reported. purified enzyme showed three enzymically...
Abstract Breast cancer (BC) is still characterized by high morbidity and mortality. A specific BC subtype named triple negative (TNBC) lacks estrogen progesterone receptors (ER PR, respectively) the absence of overexpression/amplification human epidermal growth factor receptor 2 (HER2). The androgen (AR) expressed in TNBC, although its function these cancers debated. Moreover, few therapeutic options are currently available for treatment TNBC. In this study, we have used TNBC-derived...
Abstract Prostate cancer represents the major cause of cancer-related death in men and patients frequently develop drug-resistance metastatic disease. Most studies focus on hormone-resistance mechanisms related to androgen receptor mutations or acquired property prostate cells over-activate signaling pathways. Tumor microenvironment plays a critical role progression. However, mechanism involving androgen/androgen associated fibroblasts consequences for progression still remains elusive. We...
The calf uterine 17 beta-estradiol receptor is a phosphoprotein. Phosphorylation-dephosphorylation of the controlled by cytosol kinase that activates hormone binding and nuclear phosphatase inactivates this binding. This report concerns nature kinase. Highly purified uterus preinactivated was used as substrate Ca2+ calmodulin stimulate both kinase-dependent activation 32P incorporation from [gamma-32P]-ATP into receptor. Maximal stimulation requires 1 microM 0.6 calmodulin. Fifteen...
In NIH3T3 cells, 0.001 nM of the synthetic androgen R1881 induces and stimulates association receptor (AR) with Src phosphatidylinositol 3-kinase (Pl3-kinase), respectively, thereby triggering S-phase entry. 10 Rac activity membrane ruffling in absence receptor–Src–PI3-kinase complex assembly. The antiandrogen Casodex specific inhibitors PI3-kinase prevent both hormonal effects, DNA synthesis cytoskeletal changes. Neither low nor high concentration allows nuclear translocation...
Hormone binding controls the activity of estradiol receptor. The in vitro synthesized human receptor binds hormone with high affinity and low efficiency (1–4% maximal binding). We now report that phosphorylation on tyrosine synthetic by an extensively purified calf uterus kinase increases towards levels without change affinity. This is first direct demonstration a newly acquires ligand through phosphorylation. use proteins as substrates for enzymes which cause functional modifications very...
Background Androgen receptor (AR) controls male morphogenesis, gametogenesis and prostate growth as well development of cancer. These findings support a role for AR in cell migration invasiveness. However, the molecular mechanism involved AR-mediated still remains elusive. Methodology/Principal Findings Mouse embryo NIH3T3 fibroblasts highly metastatic human fibrosarcoma HT1080 cells harbor low levels transcriptionally incompetent AR. We now report that, through extra nuclear action,...
1. Nuclei of the calf uterus are endowed with an activity inactivating crude oestrogen-receptor complex. This has been partially purified. It shows a very high affinity for complex (Km = 0.8 × 10(-9) mol specific [3H]oestradiol-17 beta-binding sites/l) as well oestrogen-free receptor 1.5 beta binding sites/l). 2. The nuclear receptor-inactivating is enhanced by dithiothreitol and inhibited several phosphatase inhibitors 4-nitrophenyl phosphate, known substrate. inhibition that...
Abstract This report offers direct evidence that association of the estradiol receptor (ER) with Src triggered by steroid agonists or growth factors controls breast and prostate cancer cell growth. is abolished in whole cells vitro a six-amino-acid peptide mimics sequence around phosphotyrosine residue position 537 human ERα. The phosphorylated peptide, at nanomolar concentrations, taken up MCF-7 LNCaP derived from mammary cancers, respectively. In addition, to block ER/Src interaction,...
In breast cancer cells, cytoplasmic localization of the estradiol receptor α (ERα) regulates estradiol-dependent S phase entry. We identified a nuclear export sequence (NES) in ERα and show that its is dependent on both estradiol-mediated phosphatidylinositol-3-kinase (PI3K)/AKT activation chromosome region maintenance 1 (CRM1). A Tat peptide containing NES disrupts ERα–CRM1 interaction prevents ERα- estradiol-induced DNA synthesis. NES-ERα mutants do not exit nucleus inhibit entry;...
Abstract Acid α-glucosidase has been isolated from cattle liver in ultracentrifugally and electrophoretically homogeneous form partially characterized. The enzyme a sedimentation constant of 5.7 S, diffusion 4.7 x 10-7 cm2. sec-1, an average molecular weight, as determined by three different methods, 107,000. contains four —SH groups per molecule, it is progressively inactivated incubation at pH above 7, the inactivation being prevented sulfhydryl group reagents. highly purified catalyzes...
Abstract Malignant melanoma represents the fifth most common cancer in world and its incidence is rising. Novel therapies targeting receptor tyrosine kinases, kinases immune checkpoints have been employed with a significant improvement of overall survival long-term disease containment. Nevertheless, often progresses becomes resistant to therapies. As such, discovery new targets drugs for advanced still remains difficult task. Gender disparities, female advantage outcome, reported. Although...