A5041111657- Cellular Mechanics and Interactions
- ATP Synthase and ATPases Research
- Heat shock proteins research
- Cell Image Analysis Techniques
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- Cancer Cells and Metastasis
- Galectins and Cancer Biology
- Digestive system and related health
- Biomedical Text Mining and Ontologies
- Image Processing Techniques and Applications
- Digital Holography and Microscopy
- Celiac Disease Research and Management
- Cancer, Stress, Anesthesia, and Immune Response
- Chronic Myeloid Leukemia Treatments
- Enzyme Structure and Function
- Thyroid Cancer Diagnosis and Treatment
- Bioinformatics and Genomic Networks
- 3D Printing in Biomedical Research
- SARS-CoV-2 detection and testing
- Gene Regulatory Network Analysis
- Protein purification and stability
- Chemokine receptors and signaling
- Wound Healing and Treatments
- Plant biochemistry and biosynthesis
University of Naples Federico II
2010-2024
Ceinge Biotecnologie Avanzate (Italy)
2013-2022
National Research Council
2005
Background Androgen receptor (AR) controls male morphogenesis, gametogenesis and prostate growth as well development of cancer. These findings support a role for AR in cell migration invasiveness. However, the molecular mechanism involved AR-mediated still remains elusive. Methodology/Principal Findings Mouse embryo NIH3T3 fibroblasts highly metastatic human fibrosarcoma HT1080 cells harbor low levels transcriptionally incompetent AR. We now report that, through extra nuclear action,...
Abstract Background Protein kinases are a well defined family of proteins, characterized by the presence common kinase catalytic domain and playing significant role in many important cellular processes, such as proliferation, maintenance cell shape, apoptosys. In members family, additional non-kinase domains contribute further specialization, resulting subcellular localization, protein binding regulation activity, among others. About 500 genes encode human genome, although them represent...
Cell migration is essential in physiological and pathological processes, such as wound healing metastasis formation. Ras involvement these processes has been extensively demonstrated. This work attempts to characterize regulation of the phenomena determining directional cell by separately analyzing role its principal effector pathways, MAPK PI3K.NIH3T3 NIHRasV12 fibroblasts were followed assays study, time under a stimulus, both standard conditions presence PI3K inhibitors. Several...
Cutaneous melanoma (CM) remains one of the leading causes tumor mortality due to its high metastatic spread. CM growth is influenced by inflammation regulated prostaglandins (PGs) whose synthesis catalyzed cyclooxygenases (COXs). COX inhibitors, including non-steroidal anti-inflammatory drugs (NSAIDs), can inhibit development and growth. In particular, in vitro experiments have shown that celecoxib, a NSAID, inhibits some cell lines. However, two-dimensional (2D) cultures, used traditional...
Abstract Background Cell motility plays a central role in development, wound-healing and tumour invasion. Cultures of eucariotic cells are complex system where most move according to 'random' patterns, but may also be induced more coordinate migration by means specific stimuli, such as the presence chemical attractants or introduction mechanical stimulus. Various tools have been developed that work keeping track paths followed objects performing statistical analysis on recorded path data....
Cellular movement is essential for many vital biological functions where it plays a pivotal role both at the single cell level, such as during division or differentiation, and macroscopic level within tissues, coordinated migration crucial proper morphogenesis. It also has an impact on various pathological processes, one all, cancer spreading. Cell complex phenomenon diverse experimental methods have been developed aimed dissecting analysing its distinct facets independently. In parallel,...
Celiac disease (CD) is a frequent intestinal inflammatory occurring in genetically susceptible individuals upon gluten ingestion. Recent studies point to role CD for genes involved cell shape, adhesion and actin rearrangements, including Rho family regulator, GTPase-activating protein 31 (ARHGAP31). In this study, we investigated the morphology cytoskeletons of peripheral monocyte-derived dendritic cells (DCs) from children with controls when contact physiological substrate, fibronectin. DCs...
Chronic lymphocytic leukemia (CLL) is a heterogeneous disease, whose presentation and clinical course are highly variable. Identification of novel prognostic factors may contribute to improving the CLL classification providing indications for treatment options. The zinc finger protein ZNF224 plays key role in cell transformation, through control apoptotic survival pathways. In this study, we evaluated potential application as marker progression therapy responsiveness. To aim, analyzed...
Tracing the appearance and evolution of virus variants is essential in management COVID-19 pandemic. Here, we focus on SARS-CoV-2 spread Italian patients by using viral sequences deposited public databases a tracing procedure which used to monitor pandemic detect spreading, within infected population emergent sub-clades with potential positive selection. Analyses collection monthly samples focused Italy highlighted all main sub-trees emerging at end first year It also identified additional...
Although the simple diffusion model can effectively describe movement of eukaryotic cells on a culture surface observed at relatively low sampling frequency, higher rates more complex models are often necessary to better fit experimental data. Currently available motion paths by involving additional parameters, such as linearity or directional persistence in time. However sometimes difficulties arise it is not easy evaluate presence bias. Here we present procedure which helps solve this...
High-throughput applications on time-lapse microscopy allows us to follow the in vitro temporal and spatial evolution of cell populations; analysis those kind data will reveal motion parameters such as average speed, persistence directionality, important informations for many research therapeutic drug development or wound healing. The large quantity frames usually acquired containing multiple cells require automated methods dealing with a tracking process. In this work an either semi- fully...
Tumour invasion and metastatic dissemination depends on cell motility, a process that may be studied quantitatively evaluated by analysing multidimensional digital images. Current methods allow to keep track of the paths followed specific objects perform statistical analysis recorded data. By adapting set tools, independently developed for studying movement, work with images, motility cells transformed Ras Src oncogenes has been evaluated. Fluorescent staining in vivo have used mark membrane...