A5013773492- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Lysosomal Storage Disorders Research
- Epigenetics and DNA Methylation
- Iron Metabolism and Disorders
- Acute Myeloid Leukemia Research
- Prenatal Screening and Diagnostics
- Phytochemicals and Antioxidant Activities
- Cancer-related gene regulation
- Blood groups and transfusion
- Kruppel-like factors research
- Immunodeficiency and Autoimmune Disorders
- Coffee research and impacts
- Trypanosoma species research and implications
- Tea Polyphenols and Effects
- Gastroesophageal reflux and treatments
- MicroRNA in disease regulation
- Mycotoxins in Agriculture and Food
- Antibiotics Pharmacokinetics and Efficacy
- Neonatal Health and Biochemistry
- S100 Proteins and Annexins
- Chronic Lymphocytic Leukemia Research
- Cardiovascular Function and Risk Factors
- Genetic factors in colorectal cancer
University of Naples Federico II
2015-2025
Ceinge Biotecnologie Avanzate (Italy)
2005-2024
Federico II University Hospital
2010-2016
Although hemp seed (HS) oil is characterized by more than 80% polyunsaturated fatty acids (PUFAs), a very high ω-6-to-ω-3 ratio not popular commodity. The aim of this work was to provide useful data about the bioactive components and cannabidiolic acid content in thirteen different commercial oils. investigated HS oils showed good ω-6/ω-3 ratio, ranging from 1.71 2.27, massively differed their chlorophylls (0.041–2.64 µg/g) carotenoids contents (0.29–1.73 µg/g), as well total phenols...
Tomato (Solanum lycopersicum L.) is one of the most consumed vegetables in world; it contains high amounts antioxidant phytochemicals and essential nutrients. Although commonly fresh, more than 80% its consumption derives from processed products. Since limited information on changes bioaccessibility bioactive compounds during gastrointestinal digestion was reported, this current study aimed to monitor activity, total polyphenolic carotenoid content, vitro different typologies (n = 7) canned...
Ferroptosis is a type of programmed cell death that differs from apoptosis, autophagy, and necrosis related to several physio-pathological processes, including tumorigenesis, neurodegeneration, senescence, blood diseases, kidney disorders, ischemia–reperfusion injuries. linked iron accumulation, eliciting dysfunction antioxidant systems, which favor the production lipid peroxides, membrane damage, ultimately, death. Thus, signaling pathways evoking ferroptosis are strongly associated with...
In the central nervous system glial-derived S100B protein has been associated with inflammation via nitric oxide (NO) production. As role of enteroglial cells in inflammatory bowel disease poorly investigated humans, we evaluated association and NO production ulcerative colitis (UC). mRNA expression, inducible synthase (iNOS) were rectal biopsies from 30 controls 35 UC patients. To verify correlation between production, exposed to S100B, presence or absence specific receptor for advanced...
Enteric glial cells (EGCs) have been recently indicated as key regulators of intestinal inflammation in animals. Whether or not this is true and how these participate to inflammatory responses humans unknown.We isolated primary EGCs from human small bowel then, we purified characterized those using specific markers, such S100B fibrillary acidic protein (GFAP). To mimic an scenario, exposed exogenous stimuli, lipopolysaccharide interferon-gamma (LPS IFN-γ), alone combination, evaluate...
The transcription factor GATA-1, together with its cofactor FOG-1, regulates erythropoiesis and megakaryocytopoiesis. Mutations in the DNA or FOG-1 binding sites of N-terminal zinc finger result different illnesses. Alterations face are responsible for dyserythropoietic anemia thrombocytopenia while R216Q, only mutation identified face, induces X-linked thalassemia (XLTT). former disorder has been studied detail whereas little is known about latter since one family investigated. We a second...
Spent coffee ground (SCG) is a significant by-product generated by the industry. It considered great source of bioactive molecules well-recognized for exerting biological properties. This study aimed to implement SCG in baked foods, such as cookies (SCGc), increase their potential. A comprehensive polyphenolic fraction and SCGc using high-resolution mass spectrometry analysis was performed. Moreover, polyphenol bioaccessibility change antioxidant activity during simulated gastrointestinal...
Coffee contains human health-related molecules, namely polyphenols that possess a wide range of pharmacological functions, and their intake is associated with reduced colon cancer risk. This study aimed to assess the changes in anti-inflammatory antioxidant activity coffee after simulated gastrointestinal digestion. The evaluation intracellular reactive oxygen species (ROS) levels HT-29 cell line three vitro spectrophotometric assays were performed determine samples. Characterization...
Infant milk formulas are designed to substitute human when breastfeeding is unavailable. In addition and milk-derived products, these can be a vehicle of contaminants. this work, multiclass method based on the QuEChERS (quick, easy, cheap, effective, rugged, safe) approach was developed for simultaneous determination contaminants (n = 45), including mycotoxins veterinary drug residues, occurring in infant formulas. By using an ultra-high-performance liquid chromatography quadrupole-Orbitrap...
Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) are considered the most important mycotoxins in terms of food safety. The aim this study was to evaluate hepatotoxicity AFB1 OTA exposure Wistar rats assess beneficial effect fermented whey (FW) pumpkin (P) as functional ingredients through a proteomic approach. For experimental procedures, were fed individually or combination, with addition FW FW-P mixture during 28 days. proteomics analysis, peptides separated using LC-MS/MS-QTOF system...
Mutations in the MLH1 and MSH2 genes account for a majority of cases families with Lynch Syndrome. Germ-line mutations MSH6, PMS2 MLH3 are responsible disease minority cases, usually associated milder variable phenotypes. No germ-line MSH3 have so far been Syndrome, although it is known that impaired activity leads to partial defect mismatch repair (MMR), low levels microsatellite instability at loci dinucleotide repeats colorectal cancer (CRC), thus suggesting role carcinogenesis. To...
Agro-industrial wastes contain a large number of important active compounds which can justify their use as innovative ingredients in nutraceutical products. This study aimed to provide complete analysis molecules, namely (poly)phenols pea pods water-based extracts, through UHPLC-Q-Orbitrap HRMS methodology. Data showed that 5-caffeoylquinic acid, epicatechin, and hesperidin were the most relevant found assayed with mean value 59.87, 29.46, 19.94 mg/100 g, respectively. Furthermore, changes...
Coffee represents one of the most traditionally consumed beverages worldwide, containing a broad range human health–related compounds. According to previous studies, regular coffee consumption may display protective effects against colorectal cancer and other chronic diseases. The main goal this research was evaluate bioaccessibility phenolic content variation in antioxidant capacity three different types brews after simulated gastrointestinal digestion. This would allow elucidate how...
Gene transcription in eukaryotes is modulated by the coordinated recruitment of specific factors and chromatin-modulating proteins. Indeed, gene activation and/or repression is/are regulated histone methylation status at arginine or lysine residues. In this work, co-immunoprecipitation experiments, we demonstrate that PRMT5, a type II protein methyltransferase monomethylates symmetrically dimethylates residues, physically associated with Kruppel-like box-zinc finger ZNF224, aldolase A...
Polymorphisms of genes involved in the regulation immune response are risk factors for achalasia, but their contribution to disease pathogenesis is unknown. Nitric oxide both function and inhibitory neurotransmission.The objective this article assess association functional relevance CCTTT-inducible nitric synthase (NOS2) gene promoter polymorphism achalasia.Genomic DNA was isolated from 181 achalasia patients 220 controls. Genotyping (CCTTT)n repeats performed by PCR capillary...
GATA-1 is a key regulator of hematopoiesis. A balanced ratio its two isoforms, GATA-1FL and GATA-1S, contributes to normal hematopoiesis, whereas aberrant expression GATA-1S alters the differentiation/proliferation potential hematopoietic precursors represents poor prognostic factor in myeloid leukemia. We previously reported that over-expression correlates with high levels succinate dehydrogenase subunit C (SDHC). Alternative splicing variants SDHC transcript are over-expressed several...
// Giorgia Montano 1,2 , Karina Vidovic 2 Chiara Palladino 1 Elena Cesaro Gaetano Sodaro Concetta Quintarelli 3 Biagio De Angelis Santa Errichiello Fabrizio Pane Paola Izzo Michela Grosso Urban Gullberg and Costanzo Department of Molecular Medicine Medical Biotechnology University Naples Federico II, Naples, Italy Haematology Transfusion Medicine, BioMedical Center, Lund University, Lund, Sweden Clinical Surgery, Correspondence to: Costanzo, email: Keywords : ZNF224, chronic myeloid...