Abstract Under conditions of short-term hormone deprivation, epidermal growth factor (EGF) induces DNA synthesis, cytoskeletal changes, and Src activation in MCF-7 LNCaP cells. These effects are drastically inhibited by pure estradiol or androgen antagonists, implicating a role the steroid receptors these findings. Interestingly, EGF triggers rapid association with receptor (AR) α (ERα) cells ERβ Here, we show that, through (EGFR) erb-B2, tyrosine phosphorylation ER preassociated AR, thereby...

In NIH3T3 cells, 0.001 nM of the synthetic androgen R1881 induces and stimulates association receptor (AR) with Src phosphatidylinositol 3-kinase (Pl3-kinase), respectively, thereby triggering S-phase entry. 10 Rac activity membrane ruffling in absence receptor–Src–PI3-kinase complex assembly. The antiandrogen Casodex specific inhibitors PI3-kinase prevent both hormonal effects, DNA synthesis cytoskeletal changes. Neither low nor high concentration allows nuclear translocation...

In breast cancer cells, cytoplasmic localization of the estradiol receptor α (ERα) regulates estradiol-dependent S phase entry. We identified a nuclear export sequence (NES) in ERα and show that its is dependent on both estradiol-mediated phosphatidylinositol-3-kinase (PI3K)/AKT activation chromosome region maintenance 1 (CRM1). A Tat peptide containing NES disrupts ERα–CRM1 interaction prevents ERα- estradiol-induced DNA synthesis. NES-ERα mutants do not exit nucleus inhibit entry;...

Abstract: Epidermal growth factor (EGF) stimulates DNA synthesis and cytoskeletal rearrangement in human breast cancer (MCF‐7) prostate (LNCaP) cells. Both effects are inhibited by estrogen (ICI 182,780) androgen (Casodex) antagonists. This supports the view that crosstalk exists between EGF estradiol (ER) (AR) receptors suggests these directly involved action. Our recent work shows ER phosphorylation on tyrosine promotes association of a complex EGFR, AR/ER, kinase Src. The assembly...

Expression of a dominant negative atypical protein kinase C (aPKC), PKCζ, prevents nuclear translocation extracellular regulated 2 (ERK-2), p27 reduction, and DNA synthesis induced by estradiol in human mammary cancer-derived MCF-7 cells. aPKC action upstream these events has been analyzed. In hormone-stimulated NIH 3T3 Cos cells ectopically expressing estrogen receptor alpha (hERα), is activated phosphatidylinositol 3-kinase (PI 3-kinase) and, turn, controls the Ras/MEK-1/ERK cascade....

Synchronization of human networks is fundamental in many aspects endeavour. Recently, much research effort has been spent on analyzing how motor coordination emerges groups (from rocking chairs to violin players) and it affected by coupling structure strength. Here we uncover the spontaneous emergence leadership (based physical signaling during group interaction) as a crucial factor steering occurrence synchronization complex where individuals perform joint task. In two experiments engaging...

Existing experimental works on movement coordination in human ensembles mostly investigate situations where each subject is connected to all the others through direct visual and auditory coupling, so that unavoidable social interaction affects their level. Here, we present a novel computer-based set-up study groups as minimize influence of among participants implement different pairings between them. In doing, players can only take into consideration motion designated subset others. This...

Abstract The mechanisms underlying the emergence of leadership in multi-agent systems are under investigation many areas research where group coordination is involved. Nonverbal has been mostly investigated case animal groups, and only a few works address problem human ensembles, e.g. pedestrian walking, dance. In this paper we study specific scenario small walking group. Our aim to propose rigorous mathematical methodology capable unveiling when leader or follower roles not designated...

Social robotics is an emerging field that expected to grow rapidly in the near future. In fact, it increasingly more frequent have robots operate close proximity with humans or even collaborate them joint tasks. this context, investigation of how endow a humanoid robot social behavioral skills typical human-human interactions still open problem. Among countless cues needed establish natural attunement, article reports our research toward implementation mechanism for estimating gaze...

Designing artificial avatars able to interact with humans in a safe, smart, and natural way is current open problem control. Solving such an issue will allow the design of cyber-agents capable cooperatively interacting people order fulfil common joint tasks multitude different applications. This particularly relevant context healthcare Indeed, use for rehabilitation has been proposed agents coordinate their movements those patients suffering from social or motor disorders. Moreover, it also...

Sense of Agency (SoA) is the feeling control over one's actions and their outcomes. A well-established implicit measure SoA temporal interval estimation paradigm, in which participants estimate time between a voluntary action its sensory consequence. In present study, we aimed to investigate whether valence outcome modulated SoA. The was manipulated through interaction partner's (i) positive/negative facial expression, or (ii) type gaze (gaze contact averted gaze). partner humanoid robot...

In many joint-action scenarios, humans and robots have to coordinate their movements accomplish a given shared task. Examples include lifting an object together, sawing wood log, transferring objects from point another. While dyadic coordination between human robot has been studied in previous investigations, the multi-agent scenario which be integrated into group still remains less explored field of research. this paper we discuss how synthesise artificial agent, driven by control...

Performing joint interaction requires constant mu-tual monitoring of own actions and their effects on the other's behaviour. Such an action-effect is boosted by social cues might result in increasing sense agency. Joint attention are strictly correlated both them contribute to formation a precise temporal coordination. In human-robot interaction, robot's ability establish with human partner exploit various react accordingly crucial step creating communicative robots. Along component,...

A small primary or secondary tumor load can occasionally induce more deleterious effects than a histologically identical larger one. In the four murine models studied herein this enhanced aggressiveness could not be attributed to NRAS mutations other hereditary changes, differential vascularization of live tissues, necrosis content. Instead, main feature associated with aggressive behavior was presence high number vessels, sometimes filled inflammatory cells, inside area, which we have...

In many real-word scenarios, humans and robots are required to coordinate their movements in joint tasks fulfil a common goal. While several examples regarding dyadic human robot interaction exist the current literature, multi-agent scenarios which one or more artificial agents need interact with still seldom investigated. this paper we address problem of synthesizing an autonomous agent perform paradigmatic oscillatory task ensembles while exhibiting some desired kinematic features. We...

Leadership is a complex yet fascinating phenomenon crucial in many application where coordination of multi-agent systems involved. This particularly true the case animal groups and other natural including human ensembles where, for example orchestras, leader often selected to steer group towards some desired coordinated behaviour. In this paper we study emergence leadership networks heterogeneous Kuramoto oscillators, which offer good model capturing describing performing joint oscillatory...

The interaction of robots or physical/virtual avatars with humans will be increasingly common in a number different scenarios intended to improve the quality human life. For example, domain healthcare, they can support therapists aiding patients need motor rehabilitation and so on. In this context, fundamental control problem is synthesize strategies make artificial agents interact "natural" human-like fashion. This particularly relevant when are required coordinate their motion performing...

Movement coordination in human ensembles has been studied little the current literature. In existing experimental works, situations where all subjects are connected with each other through direct visual and auditory coupling, social interaction affects their coordination, have investigated. Here, we study via a novel computer-based set-up that enables individuals to coordinate other's motion from distance so as minimize influence of interaction. The proposed platform makes it possible...

This research report introduces a learning system designed to detect the object that humans are gazing at, using solely visual feedback. By incorporating face detection, human attention prediction, and online enables robot perceive interpret gaze accurately, thereby facilitating establishment of joint with partners. Additionally, novel dataset collected humanoid iCub is introduced, comprising more than 22,000 images from ten participants at different annotated objects. serves as benchmark...