We investigate the uptake of a nontargeted contrast agent by breast tumors using continuous wave diffuse optical tomography apparatus. The instrument operates in near‐infrared spectral window and employs 16 sources detectors to collect light parallel on surface tumor‐bearing (coronal geometry). In our protocol an extrinsic agent, Indocyanine Green (ICG), was injected bolus. Three clinical scenarios with three different pathologies were investigated. A two‐compartment model used analyze...

Fluorescence lifetime imaging (FLI) provides unique quantitative information in biomedical and molecular biology studies but relies on complex data-fitting techniques to derive the quantities of interest. Herein, we propose a fit-free approach FLI image formation that is based deep learning (DL) quantify fluorescence decays simultaneously over whole at fast speeds. We report neural network (DNN) architecture, named (FLI-Net) designed trained for different classes experiments, including...

Macroscopic fluorescence lifetime imaging (MFLI) via compressed sensed (CS) measurements enables efficient and accurate quantification of molecular interactions in vivo over a large field view (FOV). However, the current data-processing workflow is slow, complex performs poorly under photon-starved conditions. In this paper, we propose Net-FLICS, novel image reconstruction method based on convolutional neural network (CNN), to directly reconstruct intensity images from raw time-resolved CS...

Mesoscopic fluorescence molecular tomography enables longitudinal study of 3D glioblastoma model in response to drug treatment.

Diffuse optical tomography (DOT) poses a typical ill-posed inverse problem with limited number of measurements and inherently low spatial resolution. In this paper, we propose hierarchical Bayesian approach to improve resolution quantitative accuracy by using priori information provided secondary high anatomical imaging modality, such as magnetic resonance (MR) or x-ray. dual approach, while the correlation between images may be high, it is not perfect. For example, tumour present in image,...

A new optical acquisition scheme based on a pair of digital micromirror devices is developed and applied to three-dimensional tomographic imaging turbid media. By using pairs illumination-detection patterns with single detector, we were able perform high-resolution quantitative volumetric absorption heterogeneities embedded in optically thick samples. Additionally, reconstruction algorithm was implemented graphical processor unit provide reconstructions at frame rate 2 Hz. The structured...

Time-resolved fluorescence optical tomography allows 3-dimensional localization of multiple fluorophores based on lifetime contrast while providing a unique data set for improved resolution. However, to employ the full time measurements, light propagation model that accurately simulates weakly diffused and scattered photons is required. In this article, we derive computationally efficient Monte Carlo method compute time-gated Jacobians simultaneous imaging two with contrast. The formulation...

In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance patterns with a imaging platform is investigated in vitro and small animal model. A Monte Carlo-based forward model employed to reconstruct yield based on time-gated datasets. An improvement resolution quantification when time-gate data type compared commonly used cw demonstrated vitro. Furthermore, strategies preclinical...

The conjugation of anti-cancer drugs to endogenous ligands has proven be an effective strategy enhance their pharmacological selectivity and delivery towards neoplasic tissues. Since cell proliferation a strong requirement for iron, cancer cells express high levels transferrin receptors (TfnR), making its ligand, (Tfn), great interest as agent therapeutics. However, critical gap exists in the ability non-invasively determine whether conjugated Tfn are internalized into target vivo. Due...

The Monte Carlo method is an accurate model for time-resolved quantitative fluorescence tomography. However, this suffers from low computational efficiency due to the large number of photons required reliable statistics. This paper presents a comparison study on three Carlo-based methods time-domain molecular tomography.The investigated generate time-gated Jacobians were perturbation (pMC) method, adjoint (aMC) and mid-way (mMC) method. effects different parameters that affect computation...

The design and characterization of a time-resolved functional imager using wide-field excitation scheme for small animal imaging is described. optimal operation parameters are established based on phantom studies. performance the platform simultaneous 3D reconstruction absorption scattering coefficients investigated in vitro.

Time domain fluorescence molecular tomography (TD-FMT) provides a unique dataset for enhanced quantification and spatial resolution. The time-gate can be divided into two temporal groups around the maximum counts gate, which are early gates late gates. It is well established that allow improved resolution essential fluorophore unmixing concentration quantification. However, inverse problem of FMT ill-posed typically underdetermined, makes image reconstruction highly susceptible to data...

Measuring motor skill proficiency is critical for the certification of highly skilled individuals in numerous fields. However, conventional measures use subjective metrics that often cannot distinguish between expertise levels. We present an advanced optical neuroimaging methodology can objectively and successfully classify subjects with different levels associated bimanual dexterity. The was tested by assessing laparoscopic surgery skills within framework fundamentals a program, which...

Forster resonance energy transfer (FRET) is a nonradiative of between two fluorescent molecules (a donor and an acceptor) in nanometer range proximity.FRET imaging methods have been applied to proteomic studies drug discovery applications based on intermolecular FRET efficiency measurements stoichiometric interaction as quantitative parameters interest.Importantly, provides information about biomolecular interactions at molecular level, well beyond the diffraction limits standard microscopy...

Perturbation Monte Carlo (pMC) has been previously proposed to rapidly recompute optical measurements when small perturbations of properties are considered, but it was largely restricted changes associated with prior tissue segments or regions-of-interest. In this work, we expand pMC compute spatially and temporally resolved sensitivity profiles, i.e. the Jacobians, for diffuse tomography (DOT) applications. By recording pseudo random number generator (PRNG) seeds each detected photon, able...

We present a time-resolved fluorescence diffuse optical tomography platform that is based on wide-field structured illumination, single-pixel detection, and hyperspectral acquisition. Two spatial light modulators (digital micro-mirror devices) are employed to generate independently illumination detection patterns, coupled with 16-channel spectrophotometer module capture tomographic data sets. The main system characteristics reported, we demonstrate the feasibility of acquiring dense 4D sets...

Optical imaging assays, especially fluorescence molecular are minimally invasive if not completely noninvasive, and thus an ideal technique to be applied live specimens. These assays a powerful tool in biomedical sciences as they allow the study of wide range physiological events occurring biological systems. Furthermore, optical bridge gap between vitro cell-based analysis subcellular processes vivo disease mechanisms small animal models. In particular, application Förster resonance energy...