A5077484421- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Hippo pathway signaling and YAP/TAZ
- NF-κB Signaling Pathways
- Mitochondrial Function and Pathology
- interferon and immune responses
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Advanced Fluorescence Microscopy Techniques
- Wnt/β-catenin signaling in development and cancer
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Retinal Diseases and Treatments
- Optical Coherence Tomography Applications
- Immune Cell Function and Interaction
- Fibroblast Growth Factor Research
- Advanced Biosensing Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Vascular Malformations Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Neurofibromatosis and Schwannoma Cases
- Retinal and Macular Surgery
- ATP Synthase and ATPases Research
Inserm
2011-2025
Université de Bourgogne
2011-2025
Université Paris Sciences et Lettres
2018-2022
Sorbonne Université
2018-2022
Institut Curie
2018-2022
Université Paris Cité
2018-2022
Centre National de la Recherche Scientifique
2018-2022
University of Geneva
2014-2017
La Ligue Contre le Cancer
2016
Université Bourgogne Franche-Comté
2016
// Florent Dufour 1, 2 , Thibault Rattier Andrei Alexandru Constantinescu Luciana Zischler 2, 3 Aymeric Morlé Hazem Ben Mabrouk 4 Etienne Humblin Guillaume Jacquemin Eva Szegezdi 5 Fabien Delacote 6 Naziha Marrakchi Gilles Guichard 7 Catherine Pellat-Deceunynck 8 Pierre Vacher 9 Patrick Legembre 10 Carmen Garrido 11 Olivier Micheau 1 INSERM, UMR866, « Equipe labellisée Ligue contre le Cancer » and Laboratoire d'Excellence LipSTIC, Dijon, France Univ. Bourgogne Franche-Comté, Pós-graduação...
Background TRAIL/Apo2L is a pro-apoptotic ligand of the TNF family that engages apoptotic machinery through two receptors, TRAIL-R1 and TRAIL-R2. This cell death program tightly controlled by antagonistic TRAIL-R3 TRAIL-R4, both devoid functional domain, an intracellular region receptor, required for recruitment activation initiator caspases. Upon TRAIL-binding, TRAIL-R4 forms heteromeric complex with agonistic receptor TRAIL-R2 leading to reduced caspase-8 apoptosis. Methodology/Principal...
A fully flexible imaging window acting as an implantable transparent “second skin” upgrades in vivo imaging.
Real-time in vivo imaging provides an essential window into the spatiotemporal cellular events contributing to tissue development and pathology. By coupling longitudinal intravital with genetic lineage tracing, here we capture earliest arising response active Wnt/β-catenin signaling ensuing impact on organization differentiation of mammary epithelium. This enables us interrogate how regulates dynamics distinct subpopulations epithelial cells real time. We show that β-catenin stabilization,...
Tumours are complex ecosystems composed of different types cells that communicate and influence each other. While the critical role stromal in affecting tumour growth is well established, impact mutant cancer on healthy surrounding tissues remains poorly defined. Here, using mouse intestinal organoids, we uncover a paracrine mechanism by which reactivate foetal regenerative YAP-associated transcriptional programmes neighbouring wildtype epithelial cells, rendering them adapted to thrive...
Abstract Colon tumours are hierarchically organized and contain multipotent self-renewing cells, called Cancer Stem Cells (CSCs). We have previously shown that the Notch1 receptor is expressed in Intestinal (ISCs); given critical role played by Notch signalling promoting intestinal tumourigenesis, we explored expression tumours. Combining lineage tracing two tumour models with transcriptomic analyses, found Notch1+ cells undifferentiated, proliferative capable of indefinite self-renewal...
Ezrin belongs to the ERM (ezrin-radixin-moesin) protein family and has been demonstrated regulate early steps of Fas receptor signalling in lymphoid cells, but its contribution TRAIL-induced cell death regulation adherent cancer cells remains unknown. In this study we report that FasL by ezrin is type dependant. a positive regulator apoptosis T-lymphoma line Jurkat, negative colon cells. Using phosphorylation or actin-binding mutants, provide evidence receptor-induced occurs cytoskeleton-...
Glioblastoma multiforme, the most aggressive primary brain tumor, is maintained by a subpopulation of glioma cells with self-renewal properties that are able to recapitulate entire tumor even after surgical resection or chemo-radiotherapy. This typifies vast heterogeneity this two extremes represented on one end stemlike (GSC) and other differentiated (GDC). Interestingly, GSC more sensitive immune effector than GDC counterpart. However, how impact killing vice versa not clear. Using newly...
Abstract Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present silicone-based window consisting fully flexible, suture-less design ideally suited for long-term, longitudinal IVM growing and tumors. Crucially, show this window, without any customization, suitable numerous anatomical locations mice using rapid standardized implantation procedure. This low-cost device represents substantial...
Abstract Tumours are complex ecosystems composed of different types cells that communicate and influence each other. While the critical role stromal in affecting tumour growth is well established, impact mutant cancer on healthy surrounding tissues remains poorly defined. Here, we uncovered a paracrine mechanism by which intestinal reactivate foetal regenerative Yap-associated transcriptional programs neighbouring wildtype epithelial cells, rendering them adapted to thrive context. We...
Abstract Real-time, in vivo imaging provides an essential window into the spatiotemporal cellular and molecular events contributing to tissue development pathology. By coupling longitudinal intravital with genetic lineage tracing, here we captured earliest underlying impact of active Wnt/β-catenin signaling on organization differentiation mammary epithelium. This enabled us interrogate how regulates dynamics distinct subpopulations epithelial cells real time. We show that β-catenin...