A5032452784- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Spectroscopy and Quantum Chemical Studies
- Light effects on plants
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Mitochondrial Function and Pathology
- Microbial Fuel Cells and Bioremediation
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Antioxidant Activity and Oxidative Stress
- Epigenetics and DNA Methylation
- Hemoglobin structure and function
- Biocrusts and Microbial Ecology
- Plant Stress Responses and Tolerance
- Electrohydrodynamics and Fluid Dynamics
- Plant biochemistry and biosynthesis
- Plant responses to elevated CO2
- CRISPR and Genetic Engineering
- ATP Synthase and ATPases Research
- Origins and Evolution of Life
- Semantic Web and Ontologies
- Protist diversity and phylogeny
Centre National de la Recherche Scientifique
2015-2024
Institut de Biologie Physico-Chimique
2016-2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2015-2024
Biologie du Chloroplaste et Perception de la Lumière chez les Microalgues
2016-2024
Vrije Universiteit Amsterdam
2018-2024
Sorbonne Université
2016-2024
Joint Center for Artificial Photosynthesis
2020-2022
University of Liège
2017
Biologie du chloroplaste et perception de la lumière chez les micro-algues
2015
Significance Photosynthetic organisms utilize sunlight as a form of energy. Under low light, they maximize their capacity to harvest photons; however, under excess dissipate part the harvested energy prevent photodamage, at expense light-use efficiency. Optimally balancing light harvesting and dissipation in natural (fluctuating light) conditions is considered target for improving productivity both algae plants. Here we have studied process green alga Chlamydomonas reinhardtii vivo. We found...
Drought is a major abiotic stress that impairs plant growth and development. Despite this, comprehensive understanding of drought effects on the photosynthetic apparatus lacking. In this work, we studied consequences 14-d treatment Arabidopsis thaliana. We used biochemical spectroscopic methods to examine membrane composition functionality. led disassembly PSII supercomplexes degradation core. The light-harvesting complexes (LHCII) instead remain in but cannot act as an antenna for active...
Whereas photosynthetic function under steady-state light conditions has been well characterized, little is known about its changes that occur in response to fluctuations. Chlororespiration, a simplified respiratory chain, widespread across all lineages, but role remains elusive. Here, we show chlororespiration plays crucial intermittent-light the green alga Chlamydomonas reinhardtii. which localized thylakoid membranes together with electron transfer involves plastoquinone reduction and...
Carotenoids are essential in oxygenic photosynthesis: they stabilize the pigment-protein complexes, active harvesting sunlight and photoprotection. In plants, present as carotenes their oxygenated derivatives, xanthophylls. While mutant plants lacking xanthophylls capable of photoautotrophic growth, no without photosystems have been reported so far, which has led to common opinion that for photosynthesis. Here, we report first plant grows photoautotrophically absence carotenes: a tobacco...
Damaged photosystem II reaction centers are the site of photoinhibition-related energy dissipation.
Light absorbed by chlorophylls of Photosystems II and I drives oxygenic photosynthesis. Light-harvesting complexes increase the absorption cross-section these photosystems. Furthermore, play a central role in photoprotection dissipating excess light energy an inducible regulated fashion. In higher plants, main light-harvesting complex is trimeric LHCII. this work, we used CRISPR/Cas9 to knockout five genes encoding LHCB1, which major component absence accumulation other LHCII isoforms was...
Computing many useful properties of Boolean formulas, such as their weighted or unweighted model count, is intractable on general representations. It can become tractable when formulas are expressed in a special form, the decision decomposable negation normal form (decision-DNNF). Knowledge compilation process converting formula into form. Unfortunately existing knowledge compilers provide no guarantee that output correctly represents original formula, and therefore they cannot validate any...
The photosynthetic apparatus must be able to withstand light conditions that exceed its capacity for carbon fixation. Photosynthetic organisms developed nonphotochemical quenching (NPQ), a process dissipates excess absorbed energy as heat and limits the production of reactive oxygen species cellular damage. In green alga Chlamydomonas reinhardtii, LHCSR pigment-binding proteins are essential NPQ. These complexes not constitutively present in thylakoid membranes; however, laboratory their...
Vascular plants use carotenoids and chlorophylls a b to harvest solar energy in the visible region (400-700 nm), but they make little of far-red (FR) light. Instead, some cyanobacteria have developed ability FR light by redesigning their photosynthetic apparatus synthesizing red-shifted chlorophylls. Implementing this strategy is considered promising increase crop yield. To prepare for this, characterization light-induced changes necessary. Here, we explore behaviour Arabidopsis thaliana...
Abstract While photosynthesis transforms sunlight energy into sugar, aerobic and anaerobic respiration (fermentation) catabolizes sugars to fuel cellular activities. These processes take place within one cell across several compartments, however it remains largely unexplored how they interact with another. Here we report that the weak acids produced during fermentation down-regulate both respiration. This effect is mechanistically explained an “ion trapping” model, in which lipid bilayer...
Chlorophyll fluorescence is a ubiquitous tool in basic and applied plant science research. Various standard commercial instruments are available for characterization of photosynthetic material like leaves or microalgae, most which integrate the overall signals above certain cut-off wavelength. However, wavelength-resolved (fluorescence appearing at different wavelengths having time dependent decay) contain vast information required to decompose complex processes into their underlying...
Abstract Nonphotochemical quenching (NPQ) mechanisms are crucial for protecting photosynthesis from photoinhibition in plants, algae, and cyanobacteria, their modulation is a long-standing goal improving crop yields. The current work demonstrates that Chlorella ohadii, green microalga thrives the desert under high light intensities fatal to many photosynthetic organisms does not perform nor require NPQ protect constant light. Instead of dissipating excess energy, it minimizes its uptake by...
Abstract Colon tumours are hierarchically organized and contain multipotent self-renewing cells, called Cancer Stem Cells (CSCs). We have previously shown that the Notch1 receptor is expressed in Intestinal (ISCs); given critical role played by Notch signalling promoting intestinal tumourigenesis, we explored expression tumours. Combining lineage tracing two tumour models with transcriptomic analyses, found Notch1+ cells undifferentiated, proliferative capable of indefinite self-renewal...
Abstract Nonphotochemical quenching (NPQ) is the process that protects photosynthetic organisms from photodamage by dissipating energy absorbed in excess as heat. In model green alga Chlamydomonas reinhardtii, NPQ abolished knock-out mutants of pigment–protein complexes LHCSR3 and LHCBM1. However, while a pH sensor switches to quenched conformation at low pH, role LHCBM1 has not been elucidated yet. this work, we combined biochemical physiological measurements study short-term high-light...
PSI is an essential component of the photosynthetic apparatus oxygenic photosynthesis. While most its subunits are conserved, recent data have shown that arrangement light-harvesting complexes I (LHCIs) differs substantially in different organisms. Here we studied PSI-LHCI supercomplex Botryococccus braunii, a colonial green alga with potential for lipid and sugar production, using functional analysis single-particle electron microscopy isolated supercomplexes complemented by time-resolved...
Abstract Cyclic electron flow (CEF), one of the major alternative transport pathways to primary linear (LEF) in chloroplasts has been discovered middle last century. It is defined as a return reductants from acceptor side Photosystem I (PSI) pool its donors via cytochrome b 6 f , and proven essential for photosynthesis. However, despite many efforts aimed at characterisation, pathway regulation CEF remain equivocal, physiological significance remains be properly defined. Here we use novel...
Abstract Non-photochemical quenching (NPQ) mechanisms are crucial for protecting photosynthesis from photoinhibition in plants, algae, and cyanobacteria, their modulation is a long-standing goal improving crop yields. The current work demonstrates that Chlorella ohadii , green micro-alga thrives the desert under high light intensities which fatal to many photosynthetic organisms, does not perform nor require NPQ protect constant light. Instead of dissipating excess energy, it minimizes its...
Abstract Photosynthesis needs to adjust dynamically changing light intensities in order maximize its efficiency, notably by the employment of alternative electron pathways. One them is chlororespiration - initially described Chlamydomonas reinhardtii . This transfer pathway, found all photosynthetic lineages, consists a reduction plastoquinone (PQ) through an NAD(P)H:PQ oxidoreductase and quinol (PQH 2 ) oxidation Plastid Terminal Oxidase, PTOX. Hence, constitutes pathway potentially...