A5042411611- ATP Synthase and ATPases Research
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Neurofibromatosis and Schwannoma Cases
- SARS-CoV-2 and COVID-19 Research
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Caveolin-1 and cellular processes
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Enzyme function and inhibition
- Ion Transport and Channel Regulation
- MicroRNA in disease regulation
- Porphyrin and Phthalocyanine Chemistry
- interferon and immune responses
- NF-κB Signaling Pathways
- Erythrocyte Function and Pathophysiology
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Adenosine and Purinergic Signaling
- Multiple Myeloma Research and Treatments
- Drug Transport and Resistance Mechanisms
Inserm
2011-2025
Université de Bourgogne
2011-2025
Istituto Superiore di Sanità
2004-2024
Centre de recherche Translationnelle en Médecine moléculaire
2010-2011
Exosomes secreted by normal and cancer cells carry deliver a variety of molecules. To date, mechanisms referring to tumor exosome trafficking, including release cell-cell transmission, have not been described. gain insight into this, exosomes purified from metastatic melanoma cell medium were labeled with lipid fluorescent probe, R18, analyzed spectrofluorometry confocal microscopy. A low pH condition is hallmark malignancy, potentially influencing uptake cells. Using different conditions as...
Background Metastatic melanoma is an untreatable cancer lacking reliable and non-invasive markers of disease progression. Exosomes are small vesicles secreted by normal as well tumor cells. Human tumor-derived exosomes involved in malignant progression we evaluated the presence plasma patients a potential tool for screening follow-up. Methodology/Principal Findings We designed in-house sandwich ELISA (Exotest) to capture quantify based on expression housekeeping proteins (CD63 Rab-5b)...
Background: Resistance to antitumor agents is a major cause of treatment failure in patients with cancer. Some mechanisms tumor resistance cytotoxic drugs may involve increased acidification extracellular compartments. We investigated whether proton pump inhibitors (PPIs), currently used the anti-acid peptic disease, could inhibit microenvironment and increase sensitivity cells agents. Methods: pretreated cell lines derived from human melanomas, adenocarcinomas, lymphomas PPIs omeprazole,...
Abstract Proton pumps like the vacuolar-type H+ ATPase (V-ATPase) are involved in control of cellular pH normal and tumor cells. Treatment with proton pump inhibitors (PPI) induces sensitization cancer cells to chemotherapeutics via modifications gradients. It is also known that low most suitable condition for a full PPI activation. Here, we tested whether treatment unbuffered culture conditions could affect survival proliferation human B-cell tumors. First, showed increased sensitivity...
Metastatic melanoma is associated with poor prognosis and still limited therapeutic options. An innovative treatment approach for this disease represented by targeting acidosis, a feature characterizing tumor microenvironment playing an important role in cancer malignancy. Proton pump inhibitors (PPI), such as esomeprazole (ESOM) are prodrugs functionally activated acidic environment, fostering pH neutralization inhibiting proton extrusion. We used human cell lines xeno-transplated SCID mice...
The phenomenon of cell cannibalism, which generally refers to the engulfment cells within other cells, was described in malignant tumors, but its biological significance is still largely unknown. In present study, we investigated occurrence, vivo relevance, and underlying mechanisms cannibalism human melanoma. As first evidence, observed that tumor clearly detectable metastatic lesions melanoma often involved T could be found a degraded state cells. Then, vitro experiments confirmed property...
Natural killer (NK) cells contribute to immunosurveillance and first-line defense in the control of tumor growth metastasis diffusion. NK cell-derived extracellular vesicles (NKEVs) are constitutively secreted, biologically active, reflect protein genetic repertoire their originating exert anti-tumor activity vitro vivo. Cancer can compromise cell functions, a status potentially reflected by EVs. Hence, NKEVs could on one hand improve cancer therapy interacting with and/or immune other sense...
Specifically targeted drug delivery systems with low immunogenicity and toxicity are deemed to increase efficacy of cancer chemotherapy. Acridine Orange (AO) is an acidophilic dye a strong tumoricidal action following excitation light source at 466 nm. However, date the clinical use AO limited by potential side effects elicited systemic administration. The endogenous nanocarrier exosomes have been recently introduced as natural system for therapeutic molecules. In this article, we show...
Background Non-Hodgkin's B-cell lymphomas account for approximately 70% of lymphomas. While its incidence is dramatically increasing worldwide, the disease still associated with high morbidity due to ineffectiveness conventional therapies, creating an urgent need novel therapeutic approaches. Unconventional compounds, including polyphenols and cytokine TRAIL, are being extensively studied their capacity restore apoptosis in a large number tumors, lymphomas.Design Methods Molecular mechanisms...
Context: Proton Pump Inhibitors (PPIs) reduce tumor acidity and therefore resistance of tumors to drugs. Carbonic Anhydrase IX (CA IX) inhibitors have proven be effective against tumors, while might impair their full effectiveness.Objective: To analyze the effect PPI/CA combined treatment human melanoma cells.Methods: The combination Lansoprazole (LAN) CA (FC9-399A S4) has been investigated in terms cell proliferation inhibition death cells.Results: these was more than single treatments both...
Background TRAIL/Apo2L is a pro-apoptotic ligand of the TNF family that engages apoptotic machinery through two receptors, TRAIL-R1 and TRAIL-R2. This cell death program tightly controlled by antagonistic TRAIL-R3 TRAIL-R4, both devoid functional domain, an intracellular region receptor, required for recruitment activation initiator caspases. Upon TRAIL-binding, TRAIL-R4 forms heteromeric complex with agonistic receptor TRAIL-R2 leading to reduced caspase-8 apoptosis. Methodology/Principal...
The membrane cytoskeleton cross-linker, ezrin, has recently been depicted as a key regulator in the progression and metastasis of several pediatric tumors. Less defined appears role ezrin human adult tumors, especially melanoma. We therefore addressed involvement metastatic phenotype melanoma cells. Our results show that cells resected from lesions patients, display marked spreading capacity SCID mice organs. Stable transfection with an deletion mutant comprising only 146 N-terminal...