A5064623322- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Nanoparticles: synthesis and applications
- Nanoparticle-Based Drug Delivery
- Anesthesia and Neurotoxicity Research
- Microtubule and mitosis dynamics
- Cancer Treatment and Pharmacology
- Toxin Mechanisms and Immunotoxins
- Lipid Membrane Structure and Behavior
- DNA Repair Mechanisms
- Alkaloids: synthesis and pharmacology
- Neurological Disease Mechanisms and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Microbial Inactivation Methods
- Berberine and alkaloids research
- Nanoplatforms for cancer theranostics
- Transgenic Plants and Applications
- Mitochondrial Function and Pathology
- Protein Interaction Studies and Fluorescence Analysis
- Air Quality and Health Impacts
- Pancreatic and Hepatic Oncology Research
- ATP Synthase and ATPases Research
- Plant responses to water stress
Istituto Superiore di Sanità
2011-2022
Omega Consult (Slovenia)
2022
University of Trento
2022
National Institute of Health
2016
Joint Research Centre
2012
Weatherford College
2002
Tumori Foundation
1995
Instituto Nazionale Tumori Regina Elena
1995
Higher Institute of Engineering
1994
Ewing sarcoma (EWS) is an aggressive bone tumor of uncertain cellular origin. CD99 a membrane protein that expressed in most cases EWS, although its function the disease unknown. Here we have shown endogenous expression modulates EWS differentiation and malignancy. We determined knocking down human cell lines reduced their ability to form tumors metastases when xenografted into immunodeficient mice diminished tumorigenic characteristics vitro. Further, reduction resulted neurite outgrowth...
Proton pump inhibitors (PPI) target tumour acidic pH and have an antineoplastic effect in melanoma. The PPI esomeprazole (ESOM) kills melanoma cells through a caspase-dependent pathway involving cytosolic acidification alkalinization of pH. In this paper, we further investigated the mechanisms ESOM-induced cell death ESOM rapidly induced accumulation reactive oxygen species (ROS) mitochondrial dysfunctions involvement NADPH oxidase. ROS scavenger N-acetyl-L-cysteine (NAC) inhibition oxidase...
// Luana Lugini 1 , Mauro Valtieri 2 Cristina Federici Serena Cecchetti 3 Stefania Meschini 4 Maria Condello Michele Signore Stefano Fais Department of Therapeutic Research and Medicine Evaluation, Istituto Superiore di Sanità, Rome, Italy Ematology, Oncology Molecular Medicine, Cell Biology Neurosciences, Technology Health, Correspondence to: Fais, email: stefano.fais@iss.it Keywords: colorectal cancer, exosomes, mesenchymal stromal cells, vacuolar H+-ATPase, CEA Received: December 16, 2015...
The mechanism whereby the morphology and connectivity of dendritic tree is regulated depends on an actin dynamics that, in turn, controlled by Rho GTPases, a family small GTP-binding proteins encompassing Rho, Rac, Cdc42 subfamilies. Cytotoxic necrotizing factor 1 (CNF1), protein toxin from Escherichia coli , constitutively activates thus leading to remodeling cytoskeleton intact cells. Here, we show that modulation cerebral RhoA Rac1 activity induced CNF1 mice leads ( i ) rearrangement...
The spatial distribution of radiation-induced DNA breaks within the cell nucleus depends on radiation quality in terms energy deposition pattern. It is generally assumed that higher linear transfer (LET), greater damage complexity. Using a combined experimental and theoretical approach, we examined phosphorylation-dephosphorylation kinetics γ-H2AX foci, size 3D focus morphology, relationship between cellular end points (i.e., killing lethal mutations) after exposure to gamma rays, protons,...
Abstract The surface and intracellular expression of mdrl‐P‐glycoprotein in parental drug‐sensitive human breast cancer cells (MCF‐7) their multidrug‐resistant (MDR) variants has been studied by using the monoclonal antibodies (MAbs) MM4.17 MRK‐16, which recognize 2 different epitopes drug efflux pump molecule. Fluorescence microscopic observations showed that P‐glycoprotein, addition to being located at cell surface, can also be found Golgi apparatus resistant cells. To confirm this...
AbstractIn our previous studies, the bisindolic alkaloid voacamine (VOA), isolated from plant Peschiera fuchsiaefolia, proved to exert a chemosensitizing effect on cultured multidrug resistant (MDR) osteosarcoma cells exposed doxorubicin (DOX). In particular, VOA was capable of inhibiting P-glycoprotein action in competitive way, thus explaining enhancement cytotoxic induced by DOX MDR cells. Afterwards, preliminary observations suggested that such an did not involve apoptotic process but...
The 2 clones, LoVo 5 and 7, derived from untreated WT human colon adenocarcinoma cells exhibiting different sensitivity to doxorubicin (DOX), were compared in order identify possible determinants of intrinsic drug resistance. A multidrug resistant variant cell line, selected by continuous exposure DOX (LoVo DX), was also included the study. Analysis expression organization cytoskeletal elements flow cytometry fluorescence microscopy evidenced a positive correlation between vimentin...
In this work we investigated the toxicological effects of nude and chemically functionalised (-NH2, -OH -COOH groups) multiwall carbon nanotubes (mwCNTs) using immortalised mouse fibroblasts cell line (Balb/3T3) as in vitro model, alternative to use animals, assess basal cytotoxicity, carcinogenic potential, genotoxicity interaction nanomaterials (NM). Combining tests such transformation assay micronucleus with physicochemical topological analysis, obtained results showing no cytotoxicity...
In 2018 there were over 1.8 million new cases worldwide of colorectal cancer and relapses after clinical treatments. Many studies ascribe the risk appearance this to Western life style : a sedentary life, obesity, low -fiber, high -fat diets can promote onset disease. Several have shown supplement phytochemicals an inhibiting effect on growth various cancers through activation apoptosis. Our goal was prove effectiveness natural compound in combined therapy cancer. Trigno M optimal candidate...
The intracellular location of the MDR1 gene product, known as P-glycoprotein (P-gp), has been detected by flow cytometry in 3 stabilized human melanoma cell lines which had never undergone cytotoxic drug treatment and did not express P-gp on plasma membrane. In addition, mRNA expression was revealed RT-PCR same lines. Immunofluorescence microscopy, performed using 2 monoclonal antibodies (MM4.17 MRK-16) employed flow-cytometric analysis, presence intracytoplasmically, a well-defined...