A5054241873- Magnesium in Health and Disease
- Plant Micronutrient Interactions and Effects
- Magnesium Alloys: Properties and Applications
- Spaceflight effects on biology
- Parathyroid Disorders and Treatments
- Protein Tyrosine Phosphatases
- Space Exploration and Technology
- Genetics, Aging, and Longevity in Model Organisms
- 3D Printing in Biomedical Research
- Distributed and Parallel Computing Systems
- Anesthesia and Neurotoxicity Research
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Therapeutic Uses of Natural Elements
- Nanoparticles: synthesis and applications
- Bone Tissue Engineering Materials
- Medical and Biological Ozone Research
- Adipokines, Inflammation, and Metabolic Diseases
- Atherosclerosis and Cardiovascular Diseases
- Reproductive biology and impacts on aquatic species
- Pluripotent Stem Cells Research
- Peroxisome Proliferator-Activated Receptors
- Scientific Computing and Data Management
- Reproductive System and Pregnancy
University of Milan
2015-2024
University of Padua
2024
Luigi Sacco Hospital
2013-2022
Mario Negri Institute for Pharmacological Research
2012-2019
IRCCS Policlinico San Donato
2013
IRCCS Istituto Auxologico Italiano
2009-2011
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2011
Magnesium (Mg) is crucial for bone health. Low concentrations of Mg inhibit the activity osteoblasts while promoting that osteoclasts, with final result inducing osteopenia. Conversely, little known about effects high extracellular on osteoclasts and osteoblasts. Since differentiation activation these cells coordinated by vitamin D₃ (VD3), we investigated Mg, as well its impact VD3 activity, in cells. U937 were induced to osteoclastic presence supra-physiological (>1 mM) Mg. The effect was...
Endothelial dysfunction is a crucial event in the early pathogenesis of cardiovascular diseases and linked to magnesium (Mg) deficiency. Indeed, endothelial cells, low Mg levels promote acquisition pro-inflammatory pro-atherogenic phenotype. This paper investigates mechanisms by which deficiency promotes oxidative stress affects behavior human umbilical vascular cells (HUVECs). Our data show that trigger initially increasing NAPDH oxidase activity then upregulating pro-oxidant...
TRPM7, a cation channel of the transient receptor potential family, has been identified as ubiquitous magnesium transporter. We here show that TRPM7 is expressed in endothelial cells isolated from umbilical vein (HUVEC), widely used model macrovascular endothelium. Quiescence and senescence do not modulate amounts, whereas oxidative stress generated by addition hydrogen peroxide increases levels. Moreover, high extracellular decreases levels activating calpains, while low magnesium, known to...
RESULTS:Ag NP are cytotoxic in a dose and time dependent fashion for HMEC.At high concentrations, Ag determine loss of membrane integrity as demonstrated by the increased activity lactate dehydrogenase culture medium.Ag rapidly stimulate formation free radicals.However, pre-incubation with Trolox, apocynin, or N-acetyl-L-cysteine, antioxidants which have different structure act through mechanisms, is not sufficient to prevent cytotoxicity.Ag also induce DNA damage dose-dependently, shown...
Infections of orthopedic implants are associated with high morbidity. The emergence antibiotic resistant strains and the tendency microbes to form biofilms on devices prompt individuation novel antimicrobial agents. Silver nanoparticles represent an interesting alternative, but their effects bone cells need be clarified. We focused osteoblast-like marrow-mesenchymal stem found that these rather cytotoxic silver nanoparticles, a half maximal inhibitory concentration around 25 µg/mL as...
Abstract Neoplastic cells accumulate magnesium, an event which provides selective advantages and is frequently associated with TRPM7overexpression. Little known about magnesium homeostasis in drug-resistant cancer cells. Therefore, we used the colon LoVo cell model compared doxorubicin-resistant to sensitive In resistant concentration of total higher while its influx capacity lower than Accordingly, express amounts TRPM6 7, both involved transport. While decreased levels are due...
The core knowledge about biomineralization is provided by studies on the advanced phases of process mainly occurring in extracellular matrix. Here, we investigate early stages evaluating chemical fingerprint initial mineral nuclei deposition intracellular milieu and their evolution toward hexagonal hydroxyapatite. study conducted human bone mesenchymal stem cells exposed to an osteogenic cocktail for 4 10 days, exploiting laboratory X-ray diffraction techniques cutting-edge developments...
Background: Magnesium deficiency contributes to atherogenesis partly by promoting the dysfunction of endothelial cells, which are critical in vascular homeostasis and diseases. Since EDF-1 PPARγ regulate crucial activities, we investigated modulation these proteins involved lipogenesis as well deposition lipids human cells cultured different concentrations magnesium. Methods: Human from umbilical vein were medium containing 0.1 5 mM magnesium for 24 h. The levels visualized Western blot....
Everolimus, an mTOR inhibitor, which has been demonstrated to induce anti-tumour effects in different types of neuroendocrine tumours, never evaluated patients with medullary thyroid cancer (MTC). The aim this study was evaluate the vitro and vivo everolimus combination octreotide MTC. Two progressive metastatic MTC high calcitonin levels were treated 5-10 mg/day. Both under treatment LAR at entry. An also performed assess on cell lines (TT MZ-CRC-1 cells). A tumour response observed both...
Exposure to real or simulated microgravity is sensed as a stress by mammalian cells, which activate complex adaptive response. In human primary endothelial we have recently shown the sequential intervention of various proteins are crucial prevent apoptosis and maintain cell function. We here demonstrate that mitophagy contributes adaptation gravitational unloading. After 4 10 d exposure in rotating wall vessel, amount BCL2 interacting protein 3, marker mitophagy, increased and, parallel,...
Culture of human endothelial cells for 10 d in real microgravity onboard the International Space Station modulated more than 1000 genes, some which are involved stress response.On Earth, 24 h after exposure to simulated microgravity, up-regulate heat shock protein (HSP) 70.To capture a broad view response gravitational unloading, we cultured primary 4 and rotating wall vessel, U.S. National Aeronautics Administration-developed surrogate system benchtop research on Earth.We highlight crucial...
Cytokinins are phytohormones critically involved in the regulation of plant growth and development. They also affect proliferation differentiation animal cells, thus representing new tools to treat diseases that involve dysfunctional cell and/or differentiation. Recently, by performing structure-function studies on human we found only N6-isopentenyladenosine its benzyl analogue N6-benzyladenosine suppress clonogenic activity different neoplastic cells. We here broaden our bladder carcinoma...
Magnesium plays a pivotal role in energy metabolism and the control of cell growth. While magnesium deprivation clearly shapes behavior normal neoplastic cells, little is known on this element differentiation. Here we show that deficiency increases transcription multipotency markers tissue-specific factors human adipose-derived mesenchymal stem cells exposed to mixture natural molecules, i.e., hyaluronic, butyric retinoid acids, which tunes We also demonstrate accelerates osteogenic...