A5000816813- Magnesium in Health and Disease
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- X-ray Spectroscopy and Fluorescence Analysis
- Microfluidic and Bio-sensing Technologies
- Trace Elements in Health
- Plant Micronutrient Interactions and Effects
- Aluminum toxicity and tolerance in plants and animals
- Molecular Sensors and Ion Detection
- Mesenchymal stem cell research
- Radiopharmaceutical Chemistry and Applications
- Magnesium Alloys: Properties and Applications
- Thallium and Germanium Studies
- Cellular Mechanics and Interactions
- Cancer therapeutics and mechanisms
- Electrochemical Analysis and Applications
- Peptidase Inhibition and Analysis
- Analytical chemistry methods development
- Nanoparticle-Based Drug Delivery
- Osteoarthritis Treatment and Mechanisms
- Synthesis and biological activity
- Geochemistry and Elemental Analysis
- RNA Interference and Gene Delivery
- Analytical Chemistry and Sensors
- Microbial Inactivation Methods
University of Bologna
2012-2019
Deutsches Diabetes-Zentrum e.V.
2018
Heinrich Heine University Düsseldorf
2018
Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are most promising candidates in current clinical trials and included several cell-therapy protocols. Together with well-known immunomodulatory regenerative potential MSC secretome, these have shown direct indirect anti-bacterial effects. However, low reproducibility standardization MSCs from different sources...
The core knowledge about biomineralization is provided by studies on the advanced phases of process mainly occurring in extracellular matrix. Here, we investigate early stages evaluating chemical fingerprint initial mineral nuclei deposition intracellular milieu and their evolution toward hexagonal hydroxyapatite. study conducted human bone mesenchymal stem cells exposed to an osteogenic cocktail for 4 10 days, exploiting laboratory X-ray diffraction techniques cutting-edge developments...
To improve pathogenetic studies in cancer development and reliable preclinical testing of anti-cancer treatments, three-dimensional (3D) cultures, including spheroids, have been widely recognized as more physiologically relevant vitro models vivo tumor behavior. Currently, the generation uniformly sized spheroids is still challenging: different 3D cell culture methods produce heterogeneous populations dimensions morphology, that may strongly influence readouts reliability correlated to...
We report a method that allows complete quantitative characterization of whole single cells, assessing the total amount carbon, nitrogen, oxygen, sodium, and magnesium providing submicrometer maps element molar concentration, cell density, mass, volume. This approach quantifying elements down to 106 atoms/μm3. result was obtained by applying multimodal fusion combines synchrotron radiation microscopy techniques with off-line atomic force microscopy. The proposed permits us find concentration...
Gathering precise information on mass density, size and weight of cells or cell aggregates, is crucial for applications in many biomedical fields with a specific focus cancer research. Although few technical solutions have been presented single-cell analysis, literature does not cover this aspect 3D models such as spheroids. Since the research interest samples notably rising, here we describe flow-apparatus, associated physical method operative protocol accurate measurements weight. The...
Magnesium plays a pivotal role in energy metabolism and the control of cell growth. While magnesium deprivation clearly shapes behavior normal neoplastic cells, little is known on this element differentiation. Here we show that deficiency increases transcription multipotency markers tissue-specific factors human adipose-derived mesenchymal stem cells exposed to mixture natural molecules, i.e., hyaluronic, butyric retinoid acids, which tunes We also demonstrate accelerates osteogenic...
Mitochondrial Mg channel MRS2 overexpression influences concentration and sensitivity to apoptosis.
The present study investigated the analytical capabilities of a new fluorescent chemosensor, named DCHQ5, phenyl derivative belonging to family diaza-crown-hydroxyquinolines, for quantitative assessment total intracellular Mg content. results obtained were compared performances DCHQ1 - parent probe series which so far was only suitable species magnesium and showed comparable atomic absorption spectroscopy. Different protocols tested in several cell lines both by flow cytometry steady state...
Abstract The processes leading to anticancer drug resistance are not completely unraveled. To get insights into the underlying mechanisms, we compared colon carcinoma cells sensitive doxorubicin with their resistant counterpart. We found that growth retarded, and show staminal ultrastructural features profoundly different from cells. phenotype is accompanied by upregulation of magnesium transporter MagT1 downregulation ion channel kinase TRPM7. demonstrate amounts TRPM7 account for...
The differing bioavailability of magnesium salts remains an open question, both at the cellular and systemic level. However, this issue is relevant for identifying most effective supplement. We compared effects three widely used salts: MgSO4, MgCl2 Mg pidolate, on proliferation four human cell types: promyelocytic leukaemia HL60, osteoblast-like Saos-2 U-2 OS, endothelial cells from umbilical vein. had no effect leukemic growth, but pidolate impaired growth in cells. In particular, cells, 1...
A newly synthesized indole-derivative is able to induce cytostatic and cytotoxic effects in the colon cancer cells HT29, effecting apoptosis by activation of an intrinsic pathway. Magnesium involved both cell growth even though its role latter process not well defined. The aims this work were: firstly, verify if magnesium content related proliferative rate HT29 cells; secondly, assess involvement cation mitochondria-mediated triggered new antiproliferative molecule. treated included...
Three-dimensional (3D) culture systems like tumor spheroids represent useful in vitro models for drug screening and more broadly cancer biology research, but the generation of uniform populations remains challenging. The possibility to properly characterize spheroid properties would increase reliability these models. To address this issue different analysis were combined: i) a new device relative analytical method accurate, simultaneous, rapid measurement mass density, weight, size...
Articular cartilage lacks intrinsic regenerative capabilities, and the current treatments fail to regenerate damaged tissue lead only temporary pain relief. These limitations have prompted development of engineering approaches, including 3D culture systems. Thanks their properties capacity recapitulate embryonic processes, spheroids obtained from mesenchymal stromal cells are increasingly studied as building blocks obtain functional tissues. The aim this study was investigate adipose...
There is growing concern about the consumption of synthetic cannabinoids (SCs), one largest groups new psychoactive substances, its consequence on human health (general population and workers), continuous placing SCs market. Although drug-induced alterations in neuronal function remain an essential component for theories drug addiction, accumulating evidence indicates important role activated astrocytes, whose pleiotropic brain physiology pathology well recognized. The study aims to clarify...
We have recently developed an optimised synthetic strategy for the preparation of chemosensors based on diaza-18-crown-6 hydroxyquinoline structure. This flexible approach allows to obtain a variety substituted derivatives with improved fluorescence, metal ion binding, uptake and localisation properties. In this context, we focused our attention design specific intracellular Zn2+ ions, because their important role in many biological processes. Interestingly, among them one particular shows...
A recent study showed the antitumor activity of a new indole-derivative – MM-67 inducing mitochondria-mediated apoptosis and decrease intracellular magnesium (Mg) concentration in HT29 colon cancer cells. Aim this work was to assess cellular Mg levels throughout MM-67-induced from early final stage process evaluate correlation with mitochondrial membrane potential (ΔΨm) variations. All analysis were performed by flow cytometry: ΔΨm assessed using...
Elemental analysis of biological sample can give information about content and distribution elements essential for human life or trace whose absence is the cause abnormal function development. However, systems contain an ensemble cells with heterogeneous chemistry elemental content; therefore, accurate characterization samples high cellular heterogeneity may only be achieved by analyzing single cells. Powerful methods in molecular biology are abundant, among them X-Ray microscopy based on...