A5076388906- Magnesium in Health and Disease
- Blood transfusion and management
- Trace Elements in Health
- X-ray Spectroscopy and Fluorescence Analysis
- Advanced X-ray Imaging Techniques
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Hemoglobinopathies and Related Disorders
- Blood donation and transfusion practices
- Parathyroid Disorders and Treatments
- Ocular Surface and Contact Lens
- Periodontal Regeneration and Treatments
- Advanced Electron Microscopy Techniques and Applications
- Blood groups and transfusion
- Mesenchymal stem cell research
- Electrochemical Analysis and Applications
- Corneal Surgery and Treatments
- Cancer, Hypoxia, and Metabolism
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Enzyme Structure and Function
- Molecular Sensors and Ion Detection
- Thallium and Germanium Studies
- COVID-19 Clinical Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Effects of Radiation Exposure
Istituti di Ricovero e Cura a Carattere Scientifico
2017-2023
Azienda Sanitaria Unità Locale di Reggio Emilia
2017-2023
Weatherford College
2023
Azienda USL di Bologna
2019
Institute for Transfusion Medicine
2018
Azienda Ospedaliera S.Maria
2017
Elettra-Sincrotrone Trieste S.C.p.A.
2015-2017
University of Bologna
2012-2016
The current status of the TwinMic beamline at Elettra synchrotron light source, that hosts European twin X-ray microscopy station, is reported. provided by a short hybrid undulator with source size and divergence intermediate between bending magnets conventional undulators, energy-tailored using collimated plane-grating monochromator. spectromicroscopy experimental station combines scanning full-field imaging in single instrument, contrast modes such as absorption, differential phase,...
Abstract Neoplastic cells accumulate magnesium, an event which provides selective advantages and is frequently associated with TRPM7overexpression. Little known about magnesium homeostasis in drug-resistant cancer cells. Therefore, we used the colon LoVo cell model compared doxorubicin-resistant to sensitive In resistant concentration of total higher while its influx capacity lower than Accordingly, express amounts TRPM6 7, both involved transport. While decreased levels are due...
We report a method that allows complete quantitative characterization of whole single cells, assessing the total amount carbon, nitrogen, oxygen, sodium, and magnesium providing submicrometer maps element molar concentration, cell density, mass, volume. This approach quantifying elements down to 106 atoms/μm3. result was obtained by applying multimodal fusion combines synchrotron radiation microscopy techniques with off-line atomic force microscopy. The proposed permits us find concentration...
Magnesium plays a pivotal role in energy metabolism and the control of cell growth. While magnesium deprivation clearly shapes behavior normal neoplastic cells, little is known on this element differentiation. Here we show that deficiency increases transcription multipotency markers tissue-specific factors human adipose-derived mesenchymal stem cells exposed to mixture natural molecules, i.e., hyaluronic, butyric retinoid acids, which tunes We also demonstrate accelerates osteogenic...
Mitochondrial Mg channel MRS2 overexpression influences concentration and sensitivity to apoptosis.
The present study investigated the analytical capabilities of a new fluorescent chemosensor, named DCHQ5, phenyl derivative belonging to family diaza-crown-hydroxyquinolines, for quantitative assessment total intracellular Mg content. results obtained were compared performances DCHQ1 - parent probe series which so far was only suitable species magnesium and showed comparable atomic absorption spectroscopy. Different protocols tested in several cell lines both by flow cytometry steady state...
Background: Although X-ray fluorescence microscopy is becoming a widely used technique for single-cell analysis, sample preparation this remains one of the main challenges in obtaining optimal conditions measurements regime. The information available to researchers on treatment inadequate and unclear, sometimes leading wasted time jeopardizing experiment’s success. Many cell fixation methods have been described, but none them systematically tested declared most suitable synchrotron...
In synchrotron facilities, imaging techniques are on high demand from the scientific community. Those related to X-ray microscopy among most prominent ones. Such include scanning transmission x-ray (STXM), full-field (TXM), and coherent diffraction (CDI) which have a wide spectrum of applications ranging clinical biomedical sciences nanotechnology cultural heritage. Their advancement is achieved through specialisation focused studies, often requiring dedicated beamline end-stations. On other...
The differing bioavailability of magnesium salts remains an open question, both at the cellular and systemic level. However, this issue is relevant for identifying most effective supplement. We compared effects three widely used salts: MgSO4, MgCl2 Mg pidolate, on proliferation four human cell types: promyelocytic leukaemia HL60, osteoblast-like Saos-2 U-2 OS, endothelial cells from umbilical vein. had no effect leukemic growth, but pidolate impaired growth in cells. In particular, cells, 1...
Background: Platelet-Rich Plasma (PRP) induces bone regeneration; however, there is low evidence supporting its efficacy in healing. The lack of a standardized protocol administration represents the main obstacle to use clinical routine for defects’ treatment. purpose this study was characterize PRP and elucidate osteogenic potential. Methods: Platelet count, fibrinogen levels, growth factors concentration were measured obtained by four apheresis procedures. HOB-01-C1, pre-osteocytic cell...
Abstract Background and Objectives The first wave of coronavirus disease‐2019 (COVID‐19) dramatically affected the Transfusion Medicine Unit Azienda Unità Sanitari Locale ‐ Istituto di Ricovero e Cura a Carattere Scientifico (AUSL‐IRCCS) Reggio Emilia, which faced total rearrangement procedures for donors patients. This study aims to assess major implications COVID‐19 on our department, focusing blood transfusion chain therapies, in order support specialists seeking efficient ways face...
A newly synthesized indole-derivative is able to induce cytostatic and cytotoxic effects in the colon cancer cells HT29, effecting apoptosis by activation of an intrinsic pathway. Magnesium involved both cell growth even though its role latter process not well defined. The aims this work were: firstly, verify if magnesium content related proliferative rate HT29 cells; secondly, assess involvement cation mitochondria-mediated triggered new antiproliferative molecule. treated included...
We present here a new methodology for quantitative mapping of light elements in cells, based on combination compositional and morphological information, derived respectively by X-ray Fluorescence Microscopy (XRFM), Atomic Force Scanning Transmission (STXM). Since XRFM (carbon, nitrogen, oxygen, sodium magnesium), are strongly influenced self-absorption, we developed an algorithm to correct this effect, using the structural information provided AFM STXM. Finally, corrected distributions have...
Within the context of Patient Blood Management (PBM) policy for peri-operative period, transfusion medicine unit our institution adopted a series strategies to support and enhance red blood cell (RBC) best practices. This study aimed evaluate appropriateness RBC therapy in post-operative before after starting multifactorial PBM policy.A 2-phase observational was conducted on patients who underwent major surgery. The designed as follows: 3 months preliminary audit, followed by policy, final...
Various ocular surface diseases are treated with blood-derived eye drops. Their use has been introduced in clinical practice because of their metabolite and growth factor content, which promotes regeneration. Blood-based drops can be prepared from different sources (i.e., whole blood or platelet apheresis donation), as well protocols (e.g., dilutions freeze/thaw cycles). This variability hampers the standardization and, consequently, evaluation efficacy. Detailing sharing methodological...
β-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM periodically receive leucodepleted packed red cells (P-RBCs) stored for maximum 14 days. The aim of this study was compare two alternative routine procedures prepare the optimal P-RBCs product, in order identify differences their content may somehow affect patients' health and quality life (QoL). In method 1, then...