A5074455550- Magnesium in Health and Disease
- Magnesium Alloys: Properties and Applications
- Trace Elements in Health
- Parathyroid Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
- Plant Micronutrient Interactions and Effects
- Pluripotent Stem Cells Research
- Aluminum toxicity and tolerance in plants and animals
- Cancer Genomics and Diagnostics
- Coronary Interventions and Diagnostics
- Ion channel regulation and function
- Hematopoietic Stem Cell Transplantation
- Nitric Oxide and Endothelin Effects
- Neuroendocrine regulation and behavior
- Hydrogen Storage and Materials
- Neuropeptides and Animal Physiology
- Polyomavirus and related diseases
University of Milan
2020-2024
University of Rome Tor Vergata
2014
Dompé (Italy)
1995
Endothelial dysfunction is a crucial event in the early pathogenesis of cardiovascular diseases and linked to magnesium (Mg) deficiency. Indeed, endothelial cells, low Mg levels promote acquisition pro-inflammatory pro-atherogenic phenotype. This paper investigates mechanisms by which deficiency promotes oxidative stress affects behavior human umbilical vascular cells (HUVECs). Our data show that trigger initially increasing NAPDH oxidase activity then upregulating pro-oxidant...
Background: Magnesium deficiency contributes to atherogenesis partly by promoting the dysfunction of endothelial cells, which are critical in vascular homeostasis and diseases. Since EDF-1 PPARγ regulate crucial activities, we investigated modulation these proteins involved lipogenesis as well deposition lipids human cells cultured different concentrations magnesium. Methods: Human from umbilical vein were medium containing 0.1 5 mM magnesium for 24 h. The levels visualized Western blot....
Magnesium (Mg) is fundamental in the brain, where it regulates metabolism and neurotransmission protects against neuroinflammation. To obtain insights into molecular basis of Mg action we investigated effects human brain organoids, a revolutionary 3D model to study neurobiology neuropathology. In particular, organoids derived from induced pluripotent stem cells were cultured presence or absence an vitro-generated blood-brain barrier (BBB), then exposed 1 5 mM concentrations inorganic organic...
Magnesium deficiency is associated with a greater risk of developing cardiovascular diseases since this cation fundamental in regulating vascular function. This clinical evidence sustained by vitro studies showing that culturing endothelial cells low concentrations magnesium promotes the acquisition pro-oxidant and pro-inflammatory phenotype. Here, we show increase reactive oxygen species low-magnesium-containing medium due to upregulation protein thioredoxin interacting (TXNIP), consequent...
The transient receptor potential cation channel subfamily M member 7 (TRPM7) is an ubiquitous fused to α-kinase domain involved in magnesium (Mg) transport, and its level of expression has been proposed as a marker endothelial function. To broaden our present knowledge about the role TRPM7 cells, we generated stable transfected Human Endothelial Cells derived from Umbilical Vein (HUVEC). TRPM7-silencing HUVEC maintain actin fibers' organization mitochondrial network. They produce reduced...
Drug-eluting bioresorbable scaffolds represent the last frontier in field of angioplasty and stenting to treat coronary artery disease, one leading causes morbidity mortality worldwide. In particular, sirolimus-eluting magnesium-based were recently introduced clinical practice. Magnesium alloys are biocompatible dissolve body fluids, thus determining high concentrations magnesium local microenvironment. Since regulates cell growth, we asked whether levels might interfere with...
A correct Magnesium (Mg 2+ ) intake is essential for bone health.In particular, Mg deficiency inhibits the proliferation of osteoblast-like SaOS-2 cells by increasing nitric oxide (NO) production through upregulation inducible NO synthase.At moment, little known about expression and role TRPM7, a channel/enzyme involved in uptake, MagT1, selective transporter, cells.Here we demonstrate that TRPM7 not modulated different extracellular concentrations its silencing exacerbates growth inhibition...
Abstract Despite remaining the best in vitro model to resemble human brain, a weakness of cerebral organoids is lack endothelial component that vivo organizes blood brain barrier (BBB). Since BBB crucial control microenvironment nervous system, this study proposes co-culture and organoids. We utilized consisting primary microvascular cells astrocytes transwell system. Starting from induced Pluripotent Stem Cells (iPSCs) we generated which were then cultured absence or presence an BBB....
Drug eluting magnesium (Mg) bioresorbable scaffolds represent a novel paradigm in percutaneous coronary intervention because Mg-based alloys are biocompatible, have adequate mechanical properties and can be resorbed without adverse events. Importantly, Mg is fundamental many biological processes, mitigates the inflammatory response beneficial for endothelium. Sirolimus widely used as an antiproliferative agent drug stents to inhibit proliferation of smooth muscle cells, thus reducing...