A5021120994- Extracellular vesicles in disease
- Toxic Organic Pollutants Impact
- Carcinogens and Genotoxicity Assessment
- Immune Cell Function and Interaction
- Effects and risks of endocrine disrupting chemicals
- Immune cells in cancer
- Parasitic Infections and Diagnostics
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Spaceflight effects on biology
- Anesthesia and Neurotoxicity Research
- RNA Research and Splicing
- Environmental Justice and Health Disparities
- Toxoplasma gondii Research Studies
- Intensive Care Unit Cognitive Disorders
- Polyamine Metabolism and Applications
- Lipid metabolism and biosynthesis
- Calcium signaling and nucleotide metabolism
- Inflammasome and immune disorders
- Hedgehog Signaling Pathway Studies
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Collagen: Extraction and Characterization
- Probiotics and Fermented Foods
Istituto Superiore di Sanità
2009-2025
University of Brescia
2021
Policlinico Tor Vergata
2010
German Cancer Research Center
2006
Heidelberg University
2006
Università degli Studi della Tuscia
1993
Natural killer (NK) cells contribute to immunosurveillance and first-line defense in the control of tumor growth metastasis diffusion. NK cell-derived extracellular vesicles (NKEVs) are constitutively secreted, biologically active, reflect protein genetic repertoire their originating exert anti-tumor activity vitro vivo. Cancer can compromise cell functions, a status potentially reflected by EVs. Hence, NKEVs could on one hand improve cancer therapy interacting with and/or immune other sense...
Recent studies on the subcellular distribution of cytoplasmic plaque proteins intercellular junctions have revealed that a number such can also occur in cyto- and nucleoplasm. This occurrence different, distant locations suggest some play roles nuclear processes addition to their involvement cell-cell adhesive interactions. Plakophilin (PKP) 3, member arm-repeat family proteins, occurs, diversity cell types, both as an architectural component plaques desmosomes dispersed particles. In...
Deformed wing virus (DWV) is a major honey bee pathogen that actively transmitted by the parasitic mite Varroa destructor and plays primary role in Apis mellifera winter colony losses. Despite intense investigation on this pollinator, which has unique environmental economic importance, mechanisms underlying molecular interactions between DWV bees are still poorly understood. Here, we report group of proteins, identified mass spectrometry, specifically co-immunoprecipitate with particles....
Research on viper venom has expanded into diverse medical applications, including cancer treatment. This study investigates the potential of Vipera ammodytes in oncology, evaluating its cytotoxicity and chemosensitising effects malignant melanoma cells. Proteomic analysis identified 125 proteins venom, with Phospholipases A2, C-type lectins, metalloproteinases among most abundant components. These are associated cytotoxic, anti-proliferative, tumor-inhibiting properties. Three cell lines...
The early detection of cutaneous melanoma, a potentially lethal cancer with rising incidence, is fundamental to increasing survival and therapeutic adjustment. In stages II–IV especially, additional indications for adjuvant therapy purposes after resection treatment metastatic patients are urgently needed. We investigated whether the fatty acid (FA) protein compositions small extracellular vesicles (sEV) derived from plasma stage 0–I, II III–IV melanoma (n = 38) could reflect disease stage....
The term cholangiocarcinoma (CCA) defines a class of epithelial malignancies originating from bile ducts. Although it has been demonstrated that CCA patients with perineural invasion (PNI) have worse prognosis, the biological features this phenomenon are yet unclear. Our data show in human intrahepatic specimens documented PNI, nerve-infiltrating cells display positivity marker cytokeratin 7, lower respect to rest tumor mass. In an vitro 3D model, move towards peripheral nerve explant...
Saposin (Sap) C is an essential cofactor for the lysosomal degradation of glucosylceramide (GC) by glucosylceramidase (GCase) and its functional impairment underlies a rare variant form Gaucher disease (GD). Sap promotes rearrangement lipid organization in membranes favoring substrate accessibility to GCase. It characterized six invariantly conserved cysteine residues involved three intramolecular disulfide bonds, which make protein remarkably stable acid environment degradation. Five...
Hereditary Spastic Paraplegia (HSP) is a neurodegenerative disease most commonly caused by autosomal dominant mutations in the SPG4 gene encoding microtubule-severing protein spastin. We hypothesise that -HSP attributable to reduced spastin function because of haploinsufficiency; thus, therapeutic approaches which elevate levels wild-type allele may be an effective therapy. However, until now, how are regulated largely unknown. Here, we show kinase HIPK2 regulates proliferating cells,...
Parabens are preservatives found in cosmetics, processed foods, and medications. The harmful repercussions on the central nervous system by one of most common parabens, propylparaben (PrP), yet unknown, especially during development. In this study, neurodevelopmental effects PrP long-term neurotoxicity were investigated zebrafish model, using an integrated approach. Zebrafish embryos exposed to two different concentrations (10 1000 μg/L), then larvae examined for their behavioral phenotypes...
Aims: The biochemistry underlying the physiological, adaptive, and toxic effects of carbon monoxide (CO) is linked to its affinity for reduced transition metals. We investigated CO signaling in vasculature, where hemoglobin (Hb), most important metal-containing carrier highly concentrated inside red blood cells (RBCs). Results: By combining NMR, MS, spectrophotometric techniques, we found that treatment whole increases concentration glutathione (GSH) RBC cytosol, which a significant Hb...
Proper chromosome segregation is crucial for preserving genomic integrity, and errors in this process cause mis-segregation, which may contribute to cancer development. Sister chromatid separation triggered by Separase, an evolutionary conserved protease that cleaves the cohesin complex, allowing dissolution of sister cohesion. Here we provide evidence Separase participates stability maintenance controlling replication fork speed. We found interacted with licensing factors MCM2-7,...
Giardiasis, a parasitic diarrheal disease caused by Giardia duodenalis, affects one billion people worldwide. Treatment relies only on restricted armamentarium of drugs. The burden and the increase in treatment failure highlight need for novel, safe well characterized drug options. antitumoral compound NBDHEX is effective vitro against trophozoites inhibits glycerol-3-phosphate dehydrogenase. Aim this work was to search additional protein targets. intrinsic fluorescence exploited proteomic...
Abstract Replication-dependent DNA double-strand breaks are harmful lesions preferentially repaired by homologous recombination (HR), a process that requires processing of ends to allow RAD51-mediated strand invasion. End resection and subsequent repair two intertwined processes, but the mechanism underlying their execution is still poorly appreciated. The WRN helicase one crucial factors for end instrumental in selecting proper pathway. Here, we reveal ordered phosphorylation CDK1, ATM ATR...
Extracellular vesicles of Natural Killer cells (NKEV) exert an antitumor effect towards hematopoietic and solid tumors have immune modulating effect, suggesting a promising role in biotherapy. In this study, continuation our former works, we demonstrated network by mass spectrometry analysis between NKEV protein cargo effects. Human healthy NKEV, both exosomes microvesicles, significant direct cytotoxic against human B cell lymphoma
Giardiasis, caused by the protozoan parasite Giardia duodenalis, is an intestinal diarrheal disease affecting almost one billion people worldwide. A small endosymbiotic dsRNA viruses, G. lamblia virus (GLV), genus Giardiavirus, family Totiviridae, might inhabit human and animal isolates of duodenalis. Three GLV genomes have been sequenced so far, only was intensively studied; moreover, a positive correlation between virulence yet to be proved. To understand biological significance infection...
Polybrominated diphenyl ethers (PBDEs) are persistent organic chemicals implied as flame retardants. Humans mainly exposed to BDE-47, -99, and -209 congeners by diet. PBDEs metabolic disruptors with the liver main target organ. To investigate their mode of action at a human-relevant concentration, we HepG2 cells these mixture 1 nM, analyzing transcriptomic proteomic profiles. KEGG pathways GSEA Hallmarks enrichment analyses evidenced that BDE-47 disrupted glucose metabolism hypoxia pathway;...
Parabens are preservatives, antimicrobials, and yeast inhibitors found in cosmetics, processed foods, medications. However, the harmful repercussions on central nervous system, especially during development, by one of most common parabens, propylparaben (PrP), yet unknown. In this study, neurodevelopmental effects PrP long-term neurotoxicity zebrafish model were investigated using an integrated approach. Zebrafish embryos exposed to two different concentrations (10 1000 µg/L) larvae examined...
Dystroglycan (DG) is a membrane receptor, belonging to the dystrophin-glycoprotein complex (DGC) and formed by two subunits, α-dystroglycan (α-DG) β-dystroglycan (β -DG). The C-terminal domain of α-DG N-terminal extracellular β -DG are connected, providing link between matrix cytosol. Under pathological conditions, such as cancer muscular dystrophies, DG may be target metalloproteinases MMP-2 MMP-9, contributing disease progression. Previously, we reported that (483–628) particularly...