A5091504046- Cancer Cells and Metastasis
- Gene Regulatory Network Analysis
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Lichen and fungal ecology
- Fungal Biology and Applications
- Liver physiology and pathology
- Algal biology and biofuel production
- Renal and related cancers
- Mycorrhizal Fungi and Plant Interactions
- Diatoms and Algae Research
- Photosynthetic Processes and Mechanisms
- Mathematical Biology Tumor Growth
Goethe University Frankfurt
2016-2023
Frankfurt Institute for Advanced Studies
2019-2021
Abstract Background Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective behaviour in mammalian organs during development, homeostasis, regeneration, pathogenesis. To investigate underlying organisation organoids, we imaged hundreds pancreas cholangiocarcinoma organoids parallel using light sheet bright-field microscopy up to 7 days. Results We quantified organoid at single-cell...
In China and other countries of East Asia, so-called Ling-zhi or Reishi mushrooms are used in traditional medicine since several centuries. Although the common practice to apply originally European name 'Ganoderma lucidum' these fungi has been questioned by taxonomists, this is still generally done recent publications with commercially cultivated strains. present study, two sold strains 'G. lucidum', M9720 M9724 from company Mycelia bvba (Belgium), compared for their fruiting body...
Abstract During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. first decision, trophectoderm and inner mass are formed. Subsequently, segregates into epiblast primitive endoderm. Inner organoids represent an experimental model system, mimicking second decision. It has been shown that of same tend to cluster stronger than expected for random Three major processes hypothesised contribute arrangements: (1) chemical signalling; (2) sorting; (3)...
Abstract Organoids are morphologically heterogeneous three-dimensional cell culture systems. To understand the organisation principles of their morphogenesis, we imaged hundreds pancreas and liver organoids in parallel using light sheet bright field microscopy for up to seven days. We quantified organoid behaviour at single-cell (microscale), individual-organoid (mesoscale), entire-culture (macroscale) levels. At resolution, monitored formation, monolayer polarisation degeneration,...
Diatom thylakoids contain much higher amounts of sulfoquinovosyl diacylglycerol (SQDG) than vascular plants and the hypothesis was brought forward that this relates to their special thylakoid structure. To test we created knock-down mutants in <i>Thalassiosira pseudonana </i>that exhibited a decreased SQDG content per cell. Surprisingly, ratio between different lipid classes did not change, pointing strict regulation composition. The antenna proteins, fucoxanthin-chlorophyll proteins (FCP),...
ABSTRACT During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. first decision, become either part of an outer trophectoderm or inner mass. Subsequently, mass (ICM) segregates into embryonic and extraembryonic lineage, giving rise to epiblast primitive endoderm, respectively. Inner organoids represent experimental model system for development, mimicking second decision taking place in vivo mouse embryos. In a previous study, spatial pattern different...