A5003277642- HIV Research and Treatment
- Digital Innovation in Industries
- Vocational Education and Training
- Education Methods and Technologies
- Ubiquitin and proteasome pathways
- Cytomegalovirus and herpesvirus research
- Cellular transport and secretion
- RNA Research and Splicing
- Sociology and Education Studies
- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- Algal biology and biofuel production
- Corporate Governance and Management
- Protein Structure and Dynamics
- HIV/AIDS drug development and treatment
- Viral-associated cancers and disorders
- RNA modifications and cancer
- Sports Science and Education
- Diatoms and Algae Research
- Photosynthetic Processes and Mechanisms
- IoT Networks and Protocols
- vaccines and immunoinformatics approaches
- Polyomavirus and related diseases
- Polymer Surface Interaction Studies
- Microfluidic and Capillary Electrophoresis Applications
Goethe University Frankfurt
2017-2023
Friedrich-Alexander-Universität Erlangen-Nürnberg
2022
FOM University of Applied Sciences for Economics and Management
2022
Gladstone Institutes
2014-2018
University of California, San Francisco
2010-2018
Quantitative BioSciences
2018
QB3
2014-2015
University of Tübingen
2008
University Hospital Heidelberg
2006-2007
Heidelberg University
2006
The small GTP binding protein Rab7 has a role in the late endocytic pathway and lysosome biogenesis. of mammalian autophagy is, however, unknown. We have addressed this by inhibiting function with RNA interference overexpression dominant negative Rab7. show here that was needed for formation preferably perinuclear, large aggregates, where autophagosome marker LC3 colocalised endosomal lysosomal markers. By electron microscopy we showed these aggregates corresponded to autophagic vacuoles...
Abstract Background The increasing availability of HIV-host interaction datasets, including both physical and genetic interactions, has created a need for software tools to integrate visualize the data. Because these host-pathogen interactions are extensive between human proteins found within many different databases, it is difficult generate integrated HIV-human networks. Results We have developed web-based platform, termed GPS-Prot http://www.gpsprot.org , that allows facile integration...
The biogenesis of the multi-subunit vacuolar-type H+-ATPase (V-ATPase) is initiated in endoplasmic reticulum with assembly proton pore V0, which controlled by a group factors. Here, we identify two hemizygous missense mutations extracellular domain accessory V-ATPase subunit ATP6AP2 (also known as [pro]renin receptor) responsible for glycosylation disorder liver disease, immunodeficiency, cutis laxa, and psychomotor impairment. We show that deficiency mouse caused hypoglycosylation serum...
Cellular restriction factors help to defend humans against human immunodeficiency virus (HIV). HIV accessory proteins hijack at least three different Cullin-RING ubiquitin ligases, which must be activated by the small ubiquitin-like protein NEDD8, in order counteract host cellular factors. We found that conjugation of NEDD8 Cullin-5 NEDD8-conjugating enzyme UBE2F is required for Vif-mediated degradation factor APOBEC3G (A3G). Pharmacological inhibition E1 MLN4924 or knockdown either its...
Transcriptional cyclin-dependent kinases (CDKs) regulate RNA polymerase II initiation and elongation as well cotranscriptional mRNA processing. In this report, we describe an important role for CDK12 in the epidermal growth factor (EGF)-induced c-FOS proto-oncogene expression mammalian cells. This kinase was found exon junction complexes (EJC) together with SR proteins thus recruited to II. cells depleted of or eukaryotic translation 4A3 (eIF4A3) from EJC, EGF induced fewer transcripts....
The endosomal sorting complex required for transport (ESCRT) is thought to support the formation of intralumenal vesicles multivesicular bodies (MVBs). ESCRT also budding HIV and has been proposed be recruited HIV‐budding site, plasma membrane T cells MVBs in macrophages. Despite increasing data on function ESCRT, ultrastructural localization its components not determined. We therefore localized four proteins machinery human macrophages by quantitative electron microscopy. All were found...
The p6 domain of human immunodeficiency virus type 1 (HIV-1) Gag has long been known to be monoubiquitinated. We have previously shown that the MA, CA, and NC domains are also monoubiquitinated at low levels (E. Gottwein H. G. Krausslich, J. Virol. 79:9134-9144, 2005). While several lines evidence support a role for ubiquitin in release, relevance ubiquitination is unclear. To directly address function ubiquitination, we constructed variants which lysine residues NC, SP2, were mutated...
ABSTRACT Moloney leukemia virus type 10 protein (MOV10) is an RNA helicase that induced by I interferon. It inhibits HIV replication at several steps of its replicative cycle. Of interest, MOV10 a component mRNA processing (P) bodies, which inhibit retrotransposition (RTP) intracisternal A particles (IAP). In this report, we studied the effects on IAP RTP and dependence P bodies. Indeed, inhibited RTP. decreased significantly not only products reverse transcriptase but also endogenous...
Ubiquitin is important for the release of human immunodeficiency virus 1 (HIV-1) and several other retroviruses. All major domains HIV-1 Gag protein are monoubiquitinated, but modifying machinery function ubiquitination remain unclear. Here, we show that induction a late budding arrest by mutation PTAP motif or specific inhibition selected ESCRT components leads to an increase Gag-ubiquitin conjugates in cells, which coincides with accumulation detergent-insoluble, multimerized at plasma...
Wire and arc additive manufacturing (WAAM) has the potential to significantly reduce material waste due milling of components made TiAl6V4 (Ti‐64). To keep up with market development, this resource‐efficient technology is becoming increasingly important achieve climate policy goals. Therefore, study not only focuses on influence different process parameters, such as torch wire feed speed, but also gas mixtures microstructure related mechanical properties, well scalability by investigating...
Diatom thylakoids contain much higher amounts of sulfoquinovosyl diacylglycerol (SQDG) than vascular plants and the hypothesis was brought forward that this relates to their special thylakoid structure. To test we created knock-down mutants in <i>Thalassiosira pseudonana </i>that exhibited a decreased SQDG content per cell. Surprisingly, ratio between different lipid classes did not change, pointing strict regulation composition. The antenna proteins, fucoxanthin-chlorophyll proteins (FCP),...