A5004620673- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- interferon and immune responses
- Respiratory viral infections research
- CRISPR and Genetic Engineering
- HIV/AIDS drug development and treatment
- SARS-CoV-2 detection and testing
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Long-Term Effects of COVID-19
- Viral Infections and Outbreaks Research
- COVID-19 epidemiological studies
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Immune responses and vaccinations
- Viral Infections and Immunology Research
- Extracellular vesicles in disease
- Influenza Virus Research Studies
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
Northwestern University
2018-2025
Quantitative BioSciences
2016-2023
Takeda (United States)
2023
Janssen (Belgium)
2023
Northwestern University
2020-2023
CSL (Switzerland)
2023
Seqirus (United States)
2023
National Institutes of Health
2023
University of California, Irvine
2023
University of California, San Francisco
2015-2022
Recent studies have profiled the innate immune signatures in patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and suggest that cellular responses to viral challenge may affect disease severity. Yet molecular events underlie recognition response SARS-CoV-2 infection remain be elucidated. Here, we find replication induces a delayed interferon (IFN) lung epithelial cells. By screening 16 putative sensors involved sensing of RNA virus infection, found MDA5 LGP2...
Successful intracellular pathogens must evade or neutralize the innate immune defenses of their host cells and render cellular environment permissive for replication. For example, to replicate efficiently in CD4(+) T lymphocytes, human immunodeficiency virus type 1 (HIV-1) encodes a protein called viral infectivity factor (Vif) that promotes pathogenesis by triggering degradation retrovirus restriction APOBEC3G. Other APOBEC3 proteins have been implicated HIV-1 restriction, but relevant...
New genetic tools are needed to understand the functional interactions between HIV and human host factors in primary cells. We recently developed a method edit genome of CD4+ T cells by electroporation CRISPR/Cas9 ribonucleoproteins (RNPs). Here, we adapted this methodology high-throughput platform for efficient, arrayed editing candidate factors. CXCR4 or CCR5 knockout generated with resistant infection tropism-dependent manner, whereas LEDGF TNPO3 results tropism-independent reduction...
Abstract Respiratory Syncytial Virus (RSV) is a leading cause of acute respiratory tract infection, with the greatest impact on infants, immunocompromised individuals, and older adults. RSV prevalence decreased substantially in United States (US) following implementation COVID-19-related non-pharmaceutical interventions but later rebounded abnormal seasonality. The biological epidemiological factors underlying this altered behavior remain poorly defined. In retrospective cohort study from...
The Vif protein of HIV-1 allows virus replication by degrading several members the host-encoded APOBEC3 family DNA cytosine deaminases. Polymorphisms in both host genes and viral vif gene have potential to impact extent among individuals. most genetically diverse seven human is APOBEC3H with known haplotypes. Overexpression studies shown that a subset these variants express stable active proteins, whereas others encode proteins short half-life little, if any, antiviral activity. We...
Disruption of cyclophilin A (CypA)-capsid interactions affects HIV-1 replication in human lymphocytes. To understand this mechanism, we utilize Jurkat cells, peripheral blood mononuclear cells (PBMCs), and CD4+ T cells. Our results show that inhibition infection caused by disrupting CypA-capsid is dependent on tripartite motif 5α (TRIM5αhu), showing TRIM5αhu restricts Accordingly, depletion rescues fail to interact with CypA, such as HIV-1-P90A. We found binds the core. affect...
ABSTRACT Background The rapid spread of SARS-CoV-2, the causative agent Coronavirus disease 2019 (COVID- 19), has been accompanied by emergence distinct viral clades, though their clinical significance remains unclear. Here, we aimed to investigate phylogenetic characteristics SARS-CoV-2 infections in Chicago, Illinois and assess relationship parameters. Methods We performed whole-genome sequencing isolates collected from COVID-19 patients a Chicago healthcare system mid-March, 2020. Using...
Abstract Background The global effort to vaccinate people against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during an ongoing pandemic has raised questions about how vaccine breakthrough infections compare with in immunologically naive individuals and the potential for vaccinated transmit virus. Methods We examined viral dynamics infectious virus shedding through daily longitudinal sampling 23 adults infected SARS-CoV-2 at varying stages of vaccination, including 6 fully...
Variants of the SARS-CoV-2 virus carry differential risks to public health. The Omicron (B.1.1.529) variant, first identified in Botswana on November 11, 2021, has spread globally faster than any previous variant concern. Understanding transmissibility is vital development health policy.
Human Immunodeficiency Virus (HIV) relies on host molecular machinery for replication. Systematic attempts to genetically or biochemically define these factors have yielded hundreds of candidates, but few been functionally validated in primary cells. Here, we target 426 genes previously implicated the HIV lifecycle through protein interaction studies CRISPR-Cas9-mediated knock-out human CD4+ T cells order systematically assess their functional roles We achieve efficient knockout (>50%...
Abstract Antiviral therapeutics to treat SARS‐CoV‐2 are needed diminish the morbidity of ongoing COVID‐19 pandemic. A well‐precedented drug target is main viral protease (M Pro ), which targeted by an approved and several investigational drugs. Emerging resistance has made new inhibitor chemotypes more pressing. Adopting a structure‐based approach, we docked 1.2 billion non‐covalent lead‐like molecules library 6.5 million electrophiles against enzyme structure. From these, 29 11 covalent...
Abstract Influenza A Virus (IAV) is a recurring respiratory virus with limited availability of antiviral therapies. Understanding host proteins essential for IAV infection can identify targets alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic protein abundance analyses using three strains (pH1N1, H3N2, H5N1) in human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions 13 IAV-modulated kinases....