A5045412808- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Endoplasmic Reticulum Stress and Disease
- T-cell and Retrovirus Studies
- interferon and immune responses
- Biomedical Research and Pathophysiology
- Advanced Proteomics Techniques and Applications
- Respiratory viral infections research
- Bone health and treatments
- Parathyroid Disorders and Treatments
Institut Pasteur
2020-2023
Centre National de la Recherche Scientifique
2020-2023
MRC University of Glasgow Centre for Virus Research
2015-2023
Université Paris Cité
2021-2022
Viruses have often evolved overlapping reading frames in order to maximize their coding capacity. Until recently, the segmented dsRNA genome of viruses Orbivirus genus was thought be monocistronic, but identification bluetongue virus (BTV) NS4 protein changed this assumption. A small ORF segment 10, NS3 +1 position, is maintained more than 300 strains 27 different BTV serotypes and 200 phylogenetically related African horse sickness (AHSV). In BTV, (named S10-ORF2 study) encodes a putative...
Interferon restricts SARS-CoV-2 replication in cell culture, but only a handful of Stimulated Genes with antiviral activity against have been identified. Here, we describe functional CRISPR/Cas9 screen aiming at identifying restriction factors. We identify DAXX, scaffold protein residing PML nuclear bodies known to limit the DNA viruses and retroviruses, as potent inhibitor SARS-CoV human cells. Basal expression DAXX is sufficient SARS-CoV-2, over-expression further infection. an early,...
Abstract Influenza A Virus (IAV) is a recurring respiratory virus with limited availability of antiviral therapies. Understanding host proteins essential for IAV infection can identify targets alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic protein abundance analyses using three strains (pH1N1, H3N2, H5N1) in human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions 13 IAV-modulated kinases....
Adaptation to mosquito vectors suited for transmission in urban settings is a major driver the emergence of arboviruses. To better anticipate future events, it crucial assess their potential adapt new vector hosts. In this work, we used two different experimental evolution approaches study adaptation process an emerging alphavirus, Mayaro virus (MAYV), Ae . aegypti , many other We identified E2-T179N as key mutation increasing MAYV replication insect cells and enhancing after escaping midgut...
Infected hosts possess two alternative strategies to protect themselves against the negative impact of virus infections: resistance, used abrogate replication, and disease tolerance, avoid tissue damage without controlling viral burden. The principles governing pathogen resistance are well understood, while less is known about those involved in tolerance. Here, we studied bluetongue (BTV), cause ruminants, as a model system investigate mechanisms virus-host interactions correlating with BTV...
Abstract Adaptation to mosquito vectors suited for transmission in urban settings is a major driver the emergence of arboviruses. To better anticipate future events, it crucial assess their potential adapt new vector hosts. In this work, we used two different experimental evolution approaches study adaptation process an emerging alphavirus, Mayaro virus (MAYV), Aedes aegypti , many We identified E2-T179N as key mutation increasing MAYV replication insect cells and enhancing by live ....
Abstract Interferon restricts SARS-CoV-2 replication in cell culture, but only a handful of Stimulated Genes with antiviral activity against have been identified. Here, we describe functional CRISPR/Cas9 screen aiming at identifying restriction factors. We identified DAXX, scaffold protein residing PML nuclear bodies known to limit the DNA viruses and retroviruses, as potent inhibitor SARS-CoV human cells. Basal expression DAXX was sufficient SARS-CoV-2, over-expression further restricted...
Bluetongue is a vector-borne disease of ruminants caused by bluetongue virus (BTV). BTV can infect essentially all domestic and wild but the clinical outcome infection differs substantially between host species. Clinical induced BTV, including haemorrhagic fever in severe cases, normally evident only sheep. Conversely, cattle are more resilient to infection, as they develop high levels viremia be reservoirs rarely show signs. Here, we concentrated on BTV-host cell interactions using primary...
ABSTRACT Infected hosts possess two alternative strategies to protect themselves against the negative impact of virus infections: (i) “resistance”, directed abrogate replication, or (ii) “disease tolerance”, aimed avoid organ and tissue damage without overly controlling viral burden. The overall principles governing pathogen resistance are well understood, while less is known about those involved in disease tolerance. Here, we studied bluetongue (BTV), cause a major ruminants, bluetongue, as...
Abstract Background Increased renal phosphate loss can be Fibroblast Growth Factor-23 (FGF-23) dependent or independent. FGF-23 reduces expression of Sodium-Phosphate co-transporters, responsible for reabsorption; and by inhibition 1-alpha hydroxylation 25-OH-D3. Reduced serum concentration 1, 25-OH2-D3 results in secondary hyperparathyroidism. Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) mutation is one the rarest causes hypophosphatemia, which hypophosphatemia. Case...