A5088389725- Mass Spectrometry Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
- ATP Synthase and ATPases Research
- Viral Infections and Vectors
- Mitochondrial Function and Pathology
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Microbial Fuel Cells and Bioremediation
- DNA and Nucleic Acid Chemistry
- Computational Drug Discovery Methods
- Disaster Response and Management
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Bacterial Identification and Susceptibility Testing
- HIV/AIDS drug development and treatment
- Microbial Community Ecology and Physiology
- Hepatitis B Virus Studies
- interferon and immune responses
- Plant Virus Research Studies
- Sepsis Diagnosis and Treatment
Otto-von-Guericke University Magdeburg
2024
Institut Pasteur
2017-2021
Centre National de la Recherche Scientifique
2020-2021
Weizmann Institute of Science
2021
Goethe University Frankfurt
2006-2018
University of Minnesota
2015-2017
Twin Cities Orthopedics
2015-2017
Mannheim University of Applied Sciences
2012-2016
Heidelberg University
2013-2016
University of St Andrews
2011-2016
A newly described coronavirus named severe acute respiratory syndrome 2 (SARS-CoV-2), which is the causative agent of disease 2019 (COVID-19), has infected over 2.3 million people, led to death more than 160,000 individuals and caused worldwide social economic disruption1,2. There are no antiviral drugs with proven clinical efficacy for treatment COVID-19, nor there any vaccines that prevent infection SARS-CoV-2, efforts develop hampered by limited knowledge molecular details how SARS-CoV-2...
ABSTRACT An outbreak of the novel coronavirus SARS-CoV-2, causative agent COVID-19 respiratory disease, has infected over 290,000 people since end 2019, killed 12,000, and caused worldwide social economic disruption 1,2 . There are currently no antiviral drugs with proven efficacy nor there vaccines for its prevention. Unfortunately, scientific community little knowledge molecular details SARS-CoV-2 infection. To illuminate this, we cloned, tagged expressed 26 29 viral proteins in human...
Targeting the main protease of SARS-CoV-2 Inside host cells, RNA genome severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is translated into two polyproteins that are cleaved to give individual viral proteins. The protease, known as Mpro or 3CLpro, plays a key role in these cleavages, making it an important drug target. Drayman et al . identified eight drugs target 3CLpro from library 1900 clinically safe drugs. Because challenge working with SARS-CoV-2, they started by screening...
Abstract SARS-CoV-2 is continually evolving, with more contagious mutations spreading rapidly. Using in vitro evolution to affinity maturate the receptor-binding domain (RBD) of spike protein towards ACE2 resulted mutations, S477N, E484K, and N501Y, be among first selected, explaining convergent “European” (20E-EU1), “British” (501.V1),”South African” (501.V2), ‘‘Brazilian” variants (501.V3). Plotting binding all RBD against their incidence population shows a strong correlation between two....
Abstract SARS-CoV-2 is the causative agent behind COVID-19 pandemic, responsible for over 170 million infections, and 3.7 deaths worldwide. Efforts to test, treat vaccinate against this pathogen all benefit from an improved understanding of basic biology SARS-CoV-2. Both viral cellular proteases play a crucial role in replication. Here, we study proteolytic cleavage proteins two cell line models replication using mass spectrometry identify protein neo-N-termini generated through protease...
Mitochondrial complex I (NADH:ubiquinone oxidoreductase) undergoes reversible deactivation upon incubation at 30-37 degrees C. The active/deactive transition could play an important role in the regulation of activity. It has been suggested recently that may become modified by S-nitrosation under pathological conditions during hypoxia or when nitric oxide:oxygen ratio increases. Apparently, a specific cysteine becomes accessible to chemical modification only deactive form enzyme. By selective...
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a widely used method in oligosaccharide analysis. Underivatized oligosaccharides are not well-suited for that purpose due to their low ionization efficiency; however, derivatization requires tedious sample purification steps which may lead losses, thereby decreasing its benefit. On-target performed by the matrix 3-aminoquinoline does require such and yields Schiff bases can be measured positive...
Bovine mitochondrial ATP synthase commonly is isolated as a monomeric complex that contains 16 protein subunits and the natural IF(1) inhibitor in substoichiometric amounts. Alternatively can be dimeric higher oligomeric states using digitonin for membrane solubilization blue native or clear electrophoresis separation of complexes. Using we could identify two synthase-associated proteins with masses smaller than 7 kDa isoelectric points close to 10 previously had been removed during...
Abstract Arthropod-borne viruses pose a major threat to global public health. Thus, innovative strategies for their control and prevention are urgently needed. Here, we exploit the natural capacity of generate defective viral genomes (DVGs) detriment. While DVGs have been described most viruses, identifying which, if any, can be used as therapeutic agents remains challenge. We present combined experimental evolution computational approach triage DVG sequence space pinpoint fittest deletions,...
Measurements of target activation in cells or tissues are key indicators efficacy during drug development. In contrast to established methods that require reagents and multiple preprocessing steps, reagent-free situ analysis engaged targets target-proximal pharmacodynamic signatures solid tumors remains challenging. Here, we demonstrate label-free quantification histone acetylation-specific mass shifts by matrix-assisted laser desorption ionization (MALDI) spectrometry biotyping can be used...
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has become a method of choice in lipid analysis, as it provides localization information for defined lipids that is not readily accessible with nonmass spectrometric methods. Most current MALDI matrices have been found empirically. Nevertheless, preferential matrix properties many analyte classes are poorly understood and may differ between classes. We used rational design semiautomated screening the discovery...
Despite many advances in membrane proteomics during the last decade fundamental problem of accessing transmembrane regions itself has only been addressed to some extent. The present study establishes a method for nano-LC-based analysis complex proteomes on basis methanolic porcine pancreatic elastase digest increase coverage. Halobacterium salinarium purple and Corynebacterium glutamicum membranes were successfully analyzed by using new protocol. We demonstrated that digests yield large...
Gastric cancer (GC) remains a malignant disease with high mortality. Patients are frequently diagnosed in advanced stages where survival prognosis is poor. Thus, there medical need to find novel drug targets and treatment strategies. Recently, the comprehensive molecular characterization of GC subtypes revealed mutations small GTPase RHOA as hallmark diffuse-type GC. activates RHO-associated protein kinases (ROCK1/2) which regulate cell contractility, migration growth thus may play role...
Transport proteins exhibiting broad substrate specificities are major determinants for the phenomenon of multidrug resistance. The Escherichia coli transporter EmrE, a 4-transmembrane, helical 12-kDa membrane protein, forms functional dimer to transport diverse array aromatic, positively charged substrates in proton/drug antiport fashion. Here, we report (13)C chemical shifts essential residue Glu(14) within binding pocket. To ensure native environment, EmrE was reconstituted into E. lipids....
Summary In bacterial c ‐type cytochromes, the haem cofactor is covalently attached via two cysteine residues organized in a ‐binding motif. Here, novel octa‐haem protein, MccA, described that contains only seven conventional motifs (CXXCH), addition to several single and conserved CH signature. Mass spectrometric analysis of purified MccA from Wolinella succinogenes suggests are actually part an unprecedented CX 15 sequence involved binding. Spectroscopic characterization identified unusual...
The cytochrome c oxidase Cox2 has been purified from native membranes of the hyperthermophilic eubacterium Aquifex aeolicus . It is a ba 3 belonging to family B heme-copper containing terminal oxidases. consists three subunits, subunit I (CoxA2, 63.9 kDa), II (CoxB2, 16.8 and an additional IIa 5.2 kDa. Surprisingly it able oxidize both reduced ubiquinol in cyanide sensitive manner. part respiratory chain supercomplex. This supercomplex contains fully assembled bc 1 complex Cox2. Although...