A5085851600- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Immune responses and vaccinations
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- Cytokine Signaling Pathways and Interactions
- Pregnancy and preeclampsia studies
- Pregnancy and Medication Impact
- Reproductive System and Pregnancy
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Neuroscience of respiration and sleep
- COVID-19 Clinical Research Studies
- CRISPR and Genetic Engineering
Duke University
2019-2023
University of North Carolina at Chapel Hill
2023
Institut Pasteur
2021
Targeting the main protease of SARS-CoV-2 Inside host cells, RNA genome severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is translated into two polyproteins that are cleaved to give individual viral proteins. The protease, known as Mpro or 3CLpro, plays a key role in these cleavages, making it an important drug target. Drayman et al . identified eight drugs target 3CLpro from library 1900 clinically safe drugs. Because challenge working with SARS-CoV-2, they started by screening...
The COVID-19 pandemic has already led to more than 700,000 deaths and innumerable changes daily life worldwide. Along with development of a vaccine, identification effective antivirals treat infected patients is the highest importance. However, rapid drug discovery requires efficient methods identify novel compounds that can inhibit virus. In this work, we present method for identifying inhibitors SARS-CoV-2 main protease, 3CL pro . This reporter-based assay allows antiviral screening in...
GPER1 in utero to the rescue! Several common pathogens, including influenza A virus (IAV), can activate systemic type I interferon (IFN) signaling during pregnancy. Such infections would be expected cause birth defects and fetal mortality, but maternal IAV rarely produce such effects, suggesting presence of a protective mechanism tissues. Harding et al. used CRISPR screen uncover IFN regulators that mediate differential control across tissues human cell lines. They found G protein–coupled...
There is an urgent need for anti-viral agents that treat SARS-CoV-2 infection. The shortest path to clinical use repurposing of drugs have established safety profile in humans. Here, we first screened a library 1,900 clinically safe inhibiting replication OC43, human beta-coronavirus causes the common-cold and relative SARS-CoV-2, identified 108 effective drugs. We further evaluated top 26 hits determined their ability inhibit as well other pathogenic RNA viruses. 20 significantly inhibited...
The interferon-stimulated transcription factor ETV7 suppresses the type I interferon response.
Middle-East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic pathogen with 36% case-fatality rate in humans. No vaccines or specific therapeutics are currently approved to use humans the camel host reservoir. Here, we computationally designed monomeric and homo-oligomeric miniproteins binding high affinity MERS-CoV spike (S) glycoprotein, main target of neutralizing antibodies vaccine development. We show that these broadly neutralize panel S variants, spanning known antigenic...
Abstract The type I interferon (IFN) response is an important component of the innate immune to viral infection. Precise control responses critical; insufficient levels interferon-stimulated genes (ISGs) can lead a failure restrict spread while excessive ISG activation result in interferon-related pathologies. While both positive and negative regulatory factors magnitude duration IFN signaling, it also appreciated that number ISGs regulate aspects themselves. However, mechanisms underlying...
ABSTRACT In late 2019 a human coronavirus, now known as SARS-CoV-2, emerged, likely from zoonotic reservoir. This virus causes COVID-19 disease, has infected millions of people, and led to hundreds thousands deaths across the globe. While best interventions control ultimately stop pandemic are prophylactic vaccines, antiviral therapeutics important limit morbidity mortality in those already infected. At this time, only one FDA approved anti-SARS-CoV-2 drug, remdesivir, is available...
Influenza A viruses encode their genomes across eight, negative sense RNA segments. The six largest segments produce mRNA transcripts that do not generally splice; however, the two smallest are actively spliced to essential viral proteins NEP and M2. Thus, utilization of splicing effectively expands coding capacity without increasing number genomic As a first step towards understanding why is more broadly utilized segments, we designed inserted an artificial intron into normally nonsplicing...
ABSTRACT Multiple coronaviruses (CoVs) can cause respiratory diseases in humans. While prophylactic vaccines designed to prevent infection are available for severe acute syndrome coronavirus-2 (SARS-CoV-2), incomplete vaccine efficacy, hesitancy, and the threat of other pathogenic CoVs which do not exist have highlighted need effective antiviral therapies. compounds targeting viral polymerase protease already clinical use, their sensitivity potential resistance mutations as well breadth...